Magnesium in PDB 1sh3: Crystal Structure of Norwalk Virus Polymerase (MGSO4 Crystal Form)

All present enzymatic activity of Crystal Structure of Norwalk Virus Polymerase (MGSO4 Crystal Form):
2.7.7.48;

The structure of Crystal Structure of Norwalk Virus Polymerase (MGSO4 Crystal Form), PDB code: 1sh3 was solved by K.K.Ng, N.Pendas-Franco, J.Rojo, J.A.Boga, A.Machin, J.M.Alonso, F.Parra, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.95
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	105.050, 109.140, 112.044, 90.00, 90.00, 90.00
R / Rfree (%)	21.5 / 28

The binding sites of Magnesium atom in the Crystal Structure of Norwalk Virus Polymerase (MGSO4 Crystal Form) (pdb code 1sh3). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Norwalk Virus Polymerase (MGSO4 Crystal Form), PDB code: 1sh3:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Norwalk Virus Polymerase (MGSO4 Crystal Form)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Norwalk Virus Polymerase (MGSO4 Crystal Form) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg600 b:39.6 occ:1.00 OD1 A:ASP344 2.2 27.6 1.0 OD2 A:ASP240 3.3 20.3 1.0 CG2 A:THR389 3.3 6.9 1.0 CG A:ASP344 3.4 18.7 1.0 O A:THR389 3.7 10.5 1.0 OD1 A:ASP240 3.8 17.6 1.0 CG A:ASP240 3.8 17.5 1.0 OD2 A:ASP344 3.9 20.3 1.0 OD1 A:ASP242 4.2 24.0 1.0 C A:THR389 4.3 7.3 1.0 O A:GLU345 4.4 15.4 1.0 N A:GLU345 4.5 16.6 1.0 CB A:THR389 4.5 7.2 1.0 CB A:ASP344 4.5 17.6 1.0 CA A:ASP344 4.6 17.8 1.0 CA A:PHE390 4.9 4.7 1.0 N A:PHE390 4.9 5.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Norwalk Virus Polymerase (MGSO4 Crystal Form)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Norwalk Virus Polymerase (MGSO4 Crystal Form) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg600 b:40.0 occ:1.00 OD1 B:ASP344 2.2 19.2 1.0 OD1 B:ASP240 2.9 22.2 1.0 CG B:ASP344 3.3 14.7 1.0 CG2 B:THR389 3.6 4.5 1.0 O B:THR389 3.6 7.9 1.0 O B:GLU345 3.7 16.5 1.0 N B:GLU345 3.8 15.9 1.0 CG B:ASP240 3.8 22.1 1.0 OD2 B:ASP240 3.9 20.9 1.0 CB B:ASP242 3.9 22.3 1.0 OD2 B:ASP344 4.0 12.8 1.0 CA B:ASP344 4.2 15.6 1.0 CB B:ASP344 4.4 14.8 1.0 C B:THR389 4.4 6.1 1.0 C B:ASP344 4.5 15.8 1.0 C B:GLU345 4.6 15.6 1.0 CA B:ASP242 4.6 22.2 1.0 CA B:GLU345 4.7 15.8 1.0 CB B:THR389 4.8 6.3 1.0

K.K.Ng, N.Pendas-Franco, J.Rojo, J.A.Boga, A.Machin, J.M.Alonso, F.Parra. Crystal Structure of Norwalk Virus Polymerase Reveals the Carboxyl Terminus in the Active Site Cleft. J.Biol.Chem. V. 279 16638 2004.
ISSN: ISSN 0021-9258
PubMed: 14764591
DOI: 10.1074/JBC.M400584200