Magnesium in PDB 1suz: The Structure of K92A Ecorv Bound to Cognate Dna and MG2+

All present enzymatic activity of The Structure of K92A Ecorv Bound to Cognate Dna and MG2+:
3.1.21.4;

The structure of The Structure of K92A Ecorv Bound to Cognate Dna and MG2+, PDB code: 1suz was solved by N.C.Horton, J.J.Perona, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.80
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	47.410, 48.790, 63.400, 97.10, 108.80, 107.30
R / Rfree (%)	19.7 / 24.4

The structure of The Structure of K92A Ecorv Bound to Cognate Dna and MG2+ also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

The binding sites of Magnesium atom in the The Structure of K92A Ecorv Bound to Cognate Dna and MG2+ (pdb code 1suz). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The Structure of K92A Ecorv Bound to Cognate Dna and MG2+, PDB code: 1suz:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The Structure of K92A Ecorv Bound to Cognate Dna and MG2+


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Structure of K92A Ecorv Bound to Cognate Dna and MG2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:21.7 occ:1.00 OD1 A:ASP90 2.0 18.7 1.0 OD1 A:ASP74 2.0 24.4 1.0 O A:HOH425 2.1 23.7 1.0 OP1 C:DA7 2.1 20.3 1.0 O A:HOH428 2.1 18.0 1.0 O C:HOH49 2.2 17.1 1.0 CG A:ASP74 2.9 23.2 1.0 CG A:ASP90 3.0 18.4 1.0 OD2 A:ASP74 3.2 28.4 1.0 P C:DA7 3.4 19.2 1.0 OD2 A:ASP90 3.4 18.5 1.0 O3' C:DT6 3.6 19.7 1.0 O C:HOH195 3.8 19.6 1.0 O A:HOH473 4.0 41.0 1.0 O A:HOH429 4.0 18.8 1.0 N A:ILE91 4.0 13.6 1.0 C5' C:DA7 4.1 20.3 1.0 O A:ILE91 4.1 13.4 1.0 CB A:ASP90 4.2 15.0 1.0 CB A:ASP74 4.3 18.4 1.0 O5' C:DA7 4.3 20.0 1.0 CA A:ASP90 4.3 14.6 1.0 N A:ASP74 4.4 14.2 1.0 OP2 C:DA7 4.5 21.1 1.0 C A:ASP90 4.7 13.6 1.0 CA A:ASP74 4.8 17.4 1.0 C A:ILE91 5.0 13.7 1.0

Magnesium binding site 2 out of 2 in the The Structure of K92A Ecorv Bound to Cognate Dna and MG2+


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Structure of K92A Ecorv Bound to Cognate Dna and MG2+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg401 b:18.7 occ:1.00 OD1 B:ASP90 2.0 17.5 1.0 OP1 D:DA7 2.0 14.7 1.0 O B:HOH414 2.1 14.2 1.0 O B:HOH413 2.1 21.3 1.0 OD2 B:ASP74 2.2 20.0 1.0 O D:HOH31 2.3 17.4 1.0 CG B:ASP74 3.0 18.7 1.0 CG B:ASP90 3.0 18.8 1.0 OD1 B:ASP74 3.2 25.3 1.0 P D:DA7 3.4 19.0 1.0 OD2 B:ASP90 3.5 23.6 1.0 O3' D:DT6 3.6 17.8 1.0 O B:HOH497 3.8 16.6 1.0 O B:HOH412 3.9 27.2 1.0 O B:HOH426 4.0 17.3 1.0 C5' D:DA7 4.0 16.3 1.0 O B:ILE91 4.1 17.6 1.0 N B:ILE91 4.2 14.8 1.0 O5' D:DA7 4.2 17.2 1.0 CB B:ASP90 4.3 14.8 1.0 CB B:ASP74 4.4 15.8 1.0 CA B:ASP90 4.4 13.7 1.0 OP2 D:DA7 4.5 19.3 1.0 N B:ASP74 4.6 11.3 1.0 O B:HOH506 4.7 40.3 1.0 C B:ASP90 4.8 14.0 1.0 O B:HOH480 4.8 41.2 1.0

N.C.Horton, J.J.Perona. Dna Cleavage By Ecorv Endonuclease: Two Metal Ions in Three Metal Ion Binding Sites. Biochemistry V. 43 6841 2004.
ISSN: ISSN 0006-2960
PubMed: 15170321
DOI: 10.1021/BI0499056