Magnesium in PDB 1t0f: Crystal Structure of the Tnsa/Tnsc(504-555) Complex

The structure of Crystal Structure of the Tnsa/Tnsc(504-555) Complex, PDB code: 1t0f was solved by D.R.Ronning, Y.Li, Z.N.Perez, P.D.Ross, A.B.Hickman, N.L.Craig, F.Dyda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.41 / 1.85
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	156.032, 70.112, 89.681, 90.00, 123.61, 90.00
R / Rfree (%)	21.1 / 23.5

The binding sites of Magnesium atom in the Crystal Structure of the Tnsa/Tnsc(504-555) Complex (pdb code 1t0f). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Tnsa/Tnsc(504-555) Complex, PDB code: 1t0f:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Tnsa/Tnsc(504-555) Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Tnsa/Tnsc(504-555) Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1010 b:30.1 occ:1.00 O A:VAL131 2.4 21.0 1.0 OD2 A:ASP114 2.4 21.8 1.0 O A:HOH2211 2.4 39.8 1.0 OE1 A:GLN130 2.5 24.2 1.0 O A:HOH2120 2.6 36.4 1.0 O A:HOH2216 2.7 36.5 1.0 CG A:ASP114 3.2 23.9 1.0 OD1 A:ASP114 3.3 28.7 1.0 CD A:GLN130 3.4 22.3 1.0 C A:VAL131 3.5 22.1 1.0 N A:VAL131 3.8 17.2 1.0 O A:HOH2208 3.9 54.1 1.0 CA A:VAL131 4.1 21.0 1.0 NZ A:LYS132 4.2 27.2 1.0 CE A:LYS132 4.2 26.2 1.0 CD A:LYS132 4.2 25.4 1.0 CB A:VAL131 4.2 22.8 1.0 O A:HOH2263 4.3 43.2 1.0 CG A:GLN130 4.3 19.9 1.0 NE2 A:GLN130 4.3 20.5 1.0 OE2 A:GLU63 4.4 27.0 1.0 O A:HOH2240 4.4 32.5 1.0 O A:HOH2238 4.6 44.1 1.0 N A:LYS132 4.6 19.9 1.0 CB A:ASP114 4.7 18.6 1.0 CG A:LYS132 4.8 22.5 1.0 C A:GLN130 4.8 18.3 1.0 CA A:LYS132 4.9 21.4 1.0 CG1 A:VAL131 4.9 26.5 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Tnsa/Tnsc(504-555) Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Tnsa/Tnsc(504-555) Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1009 b:30.1 occ:1.00 O B:VAL131 2.4 23.0 1.0 OD2 B:ASP114 2.4 27.3 1.0 OE1 B:GLN130 2.5 28.2 1.0 O B:HOH2030 2.5 28.7 1.0 O B:HOH2178 2.6 36.2 1.0 O B:HOH2223 2.7 43.8 1.0 CG B:ASP114 3.2 27.3 1.0 OD1 B:ASP114 3.3 30.3 1.0 CD B:GLN130 3.4 24.4 1.0 C B:VAL131 3.4 23.6 1.0 N B:VAL131 3.6 20.4 1.0 CA B:VAL131 3.9 22.7 1.0 O B:HOH2224 4.0 38.3 1.0 CB B:VAL131 4.1 25.1 1.0 CG B:GLN130 4.2 21.5 1.0 CE B:LYS132 4.2 29.1 1.0 NE2 B:GLN130 4.2 22.3 1.0 NZ B:LYS132 4.3 30.9 1.0 CD B:LYS132 4.3 26.9 1.0 O B:HOH2237 4.4 42.7 1.0 OE2 B:GLU63 4.4 36.6 1.0 N B:LYS132 4.6 21.8 1.0 CB B:ASP114 4.6 23.8 1.0 C B:GLN130 4.7 20.6 1.0 CG1 B:VAL131 4.7 27.8 1.0 O B:HOH2169 4.8 48.9 1.0 CG B:LYS132 4.8 26.8 1.0 O B:HOH2013 4.9 28.6 1.0 CA B:LYS132 5.0 23.6 1.0

D.R.Ronning, Y.Li, Z.N.Perez, P.D.Ross, A.B.Hickman, N.L.Craig, F.Dyda. The Carboxy-Terminal Portion of Tnsc Activates the TN7 Transposase Through A Specific Interaction with Tnsa. Embo J. V. 23 2972 2004.
ISSN: ISSN 0261-4189
PubMed: 15257292
DOI: 10.1038/SJ.EMBOJ.7600311