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Magnesium in PDB 1t9c: Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl

Enzymatic activity of Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl

All present enzymatic activity of Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl:
2.2.1.6;

Protein crystallography data

The structure of Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl, PDB code: 1t9c was solved by J.A.Mccourt, S.S.Pang, L.W.Guddat, R.G.Duggleby, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 2.34
Space group P 4 2 2
Cell size a, b, c (Å), α, β, γ (°) 154.486, 154.486, 178.796, 90.00, 90.00, 90.00
R / Rfree (%) 16.2 / 19.1

Other elements in 1t9c:

The structure of Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl also contains other interesting chemical elements:

Potassium (K) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl (pdb code 1t9c). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl, PDB code: 1t9c:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 1t9c

Go back to Magnesium Binding Sites List in 1t9c
Magnesium binding site 1 out of 2 in the Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1699

b:11.4
occ:1.00
O3B A:P221702 2.0 13.6 1.0
OD1 A:ASP550 2.1 14.9 1.0
O1A A:P221702 2.1 15.8 1.0
OD1 A:ASN577 2.1 16.8 1.0
O A:GLU579 2.2 17.6 1.0
O A:HOH4002 2.2 15.7 1.0
CG A:ASN577 3.1 15.6 1.0
PA A:P221702 3.2 16.6 1.0
PB A:P221702 3.2 14.3 1.0
O3A A:P221702 3.2 12.9 1.0
CG A:ASP550 3.2 14.9 1.0
ND2 A:ASN577 3.4 13.2 1.0
C A:GLU579 3.4 18.5 1.0
O2B A:P221702 3.8 11.3 1.0
OD2 A:ASP550 3.8 17.7 1.0
O7 A:P221702 3.8 15.8 1.0
N A:ASP550 3.8 13.3 1.0
N A:GLY581 3.9 18.8 1.0
N A:GLU579 4.0 18.6 1.0
CA A:GLU579 4.3 18.3 1.0
N A:ALA551 4.3 10.5 1.0
O2A A:P221702 4.3 13.3 1.0
N A:GLN580 4.3 19.1 1.0
CG A:GLU579 4.3 17.6 1.0
O1B A:P221702 4.4 11.1 1.0
N A:ASN577 4.4 14.6 1.0
CB A:ASP550 4.4 12.2 1.0
CA A:GLN580 4.4 20.0 1.0
O A:LEU575 4.5 16.1 1.0
CB A:ASN577 4.5 14.6 1.0
CA A:ASP550 4.6 13.3 1.0
CA A:GLY549 4.6 13.7 1.0
C A:GLY549 4.6 14.7 1.0
C A:GLN580 4.7 19.2 1.0
N A:GLU578 4.8 17.4 1.0
CA A:GLY581 4.8 19.1 1.0
CA A:ASN577 4.8 15.8 1.0
C A:ASN577 4.9 16.6 1.0
CB A:ALA551 4.9 8.6 1.0
CB A:GLU579 5.0 14.9 1.0
C A:ASP550 5.0 12.8 1.0

Magnesium binding site 2 out of 2 in 1t9c

Go back to Magnesium Binding Sites List in 1t9c
Magnesium binding site 2 out of 2 in the Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Yeast Acetohydroxyacid Synthase in Complex with A Sulfonylurea Herbicide, Sulfometuron Methyl within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg699

b:21.7
occ:1.00
O3B B:P23700 2.0 25.5 1.0
OD1 B:ASP550 2.1 18.2 1.0
O1A B:P23700 2.1 19.3 1.0
O B:HOH4006 2.2 19.2 1.0
OD1 B:ASN577 2.2 27.6 1.0
O B:GLU579 2.2 28.9 1.0
CG B:ASN577 3.0 27.6 1.0
PA B:P23700 3.1 26.1 1.0
PB B:P23700 3.2 27.6 1.0
O3A B:P23700 3.2 26.2 1.0
CG B:ASP550 3.2 17.3 1.0
ND2 B:ASN577 3.3 25.2 1.0
C B:GLU579 3.4 30.8 1.0
OD2 B:ASP550 3.7 19.7 1.0
O2B B:P23700 3.8 27.9 1.0
O7 B:P23700 3.8 26.6 1.0
N B:ASP550 3.8 16.1 1.0
N B:GLY581 3.9 31.4 1.0
N B:GLU579 4.0 30.1 1.0
O2A B:P23700 4.3 22.8 1.0
N B:ALA551 4.3 16.4 1.0
CA B:GLU579 4.3 31.3 1.0
O1B B:P23700 4.3 26.2 1.0
N B:GLN580 4.4 31.7 1.0
CG B:GLU579 4.4 32.1 1.0
CB B:ASP550 4.4 16.4 1.0
CA B:GLN580 4.4 32.1 1.0
CB B:ASN577 4.5 26.5 1.0
N B:ASN577 4.5 26.4 1.0
O B:LEU575 4.5 24.3 1.0
CA B:GLY549 4.5 17.9 1.0
C B:GLY549 4.6 17.5 1.0
CA B:ASP550 4.6 16.1 1.0
C B:GLN580 4.7 31.8 1.0
CA B:GLY581 4.7 31.2 1.0
N B:GLU578 4.8 30.0 1.0
CB B:ALA551 4.9 14.7 1.0
CA B:ASN577 4.9 27.5 1.0
C B:ASN577 4.9 29.0 1.0
C B:ASP550 5.0 18.1 1.0
C7 B:P23700 5.0 31.8 1.0

Reference:

J.A.Mccourt, S.S.Pang, L.W.Guddat, R.G.Duggleby. Elucidating the Specificity of Binding of Sulfonylurea Herbicides to Acetohydroxyacid Synthase. Biochemistry V. 44 2330 2005.
ISSN: ISSN 0006-2960
PubMed: 15709745
DOI: 10.1021/BI047980A
Page generated: Mon Dec 14 06:50:58 2020

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