Magnesium in the structure of Structural Determinants For Activation Of the Alpha-Subunit of A Heterotrimeric G Protein (pdb 1tag)

The binding sites of Magnesium atom in the structure of Structural Determinants For Activation Of the Alpha-Subunit of A Heterotrimeric G Protein (pdb code 1tag). This binding sites where shown with 5.0 Angstroms radius around Magnesium atom. The 1tag structure was solved by D.G.LAMBRIGHT, J.P.NOEL, H.E.HAMM, P.B.SIGLER, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 8.0-1.8 Space group I222 a (A) 71.900 b (A) 87.800 c (A) 143.100 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 18.7 Rfree (%) 21.1 Magnesium Binding Sites: Magnesium binding site 1 out of 1 in 1tag Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Magnesium in the PDB 1tag. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Magnesium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Lys42, A: Ser43, A: Arg174, A: Asp196, A: Gdp351, A: Hoh353, A: Hoh354, A: Hoh355, A: Hoh356, A: Hoh427, A: Hoh434, A: Hoh475, A: Hoh526, A: Hoh560, A: Hoh619, A: Hoh728, A: Hoh730, conact list: Atom Atom Distance (A) Mg CB A:Lys42 4.89 Mg C A:Lys42 4.87 Mg N A:Ser43 3.76 Mg CB A:Ser43 3.07 Mg OG A:Ser43 2.18 Mg CA A:Ser43 3.95 Mg CZ A:Arg174 4.96 Mg NH1 A:Arg174 4.01 Mg OD2 A:Asp196 4.21 Mg O3B A:Gdp351 4.35 Mg PA A:Gdp351 4.50 Mg O2A A:Gdp351 4.69 Mg O1B A:Gdp351 3.49 Mg PB A:Gdp351 3.27 Mg O2B A:Gdp351 2.18 Mg O3A A:Gdp351 4.22 Mg O1A A:Gdp351 4.06 Mg O A:Hoh353 2.20 Mg O A:Hoh354 2.21 Mg O A:Hoh355 2.14 Mg O A:Hoh356 2.14 Mg O A:Hoh427 4.74 Mg O A:Hoh434 4.41 Mg O A:Hoh475 4.20 Mg O A:Hoh526 4.26 Mg O A:Hoh560 4.12 Mg O A:Hoh619 4.91 Mg O A:Hoh728 4.44 Mg O A:Hoh730 4.67 interactive model: