Magnesium in PDB 1tb5: Catalytic Domain of Human Phosphodiesterase 4B in Complex with Amp

All present enzymatic activity of Catalytic Domain of Human Phosphodiesterase 4B in Complex with Amp:
3.1.4.17;

The structure of Catalytic Domain of Human Phosphodiesterase 4B in Complex with Amp, PDB code: 1tb5 was solved by K.Y.J.Zhang, G.L.Card, Y.Suzuki, D.R.Artis, D.Fong, S.Gillette, D.Hsieh, J.Neiman, B.L.West, C.Zhang, M.V.Milburn, S.-H.Kim, J.Schlessinger, G.Bollag, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	70.71 / 2.15
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	89.547, 94.241, 107.243, 90.00, 90.00, 90.00
R / Rfree (%)	23.4 / 26.8

The structure of Catalytic Domain of Human Phosphodiesterase 4B in Complex with Amp also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Catalytic Domain of Human Phosphodiesterase 4B in Complex with Amp (pdb code 1tb5). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Catalytic Domain of Human Phosphodiesterase 4B in Complex with Amp, PDB code: 1tb5:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Catalytic Domain of Human Phosphodiesterase 4B in Complex with Amp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Catalytic Domain of Human Phosphodiesterase 4B in Complex with Amp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1002 b:22.2 occ:1.00 O A:HOH1004 2.0 32.2 1.0 O A:HOH1003 2.0 36.2 1.0 O1P A:AMP102 2.1 30.2 0.8 OD1 A:ASP275 2.1 25.9 1.0 O A:HOH1007 2.1 36.0 1.0 O A:HOH1005 2.2 25.0 1.0 CG A:ASP275 3.1 26.6 1.0 P A:AMP102 3.3 40.6 0.8 OD2 A:ASP275 3.3 23.7 1.0 ZN A:ZN1001 3.7 33.4 1.0 O2P A:AMP102 3.7 18.3 0.8 O5' A:AMP102 3.8 40.7 0.8 O A:HOH58 4.0 45.7 1.0 OE2 A:GLU304 4.0 24.1 1.0 CE1 A:HIS307 4.1 23.4 1.0 OG1 A:THR345 4.2 27.1 1.0 O A:HIS274 4.2 23.0 1.0 ND1 A:HIS307 4.4 23.4 1.0 CD2 A:HIS274 4.5 19.3 1.0 CB A:ASP275 4.5 23.6 1.0 O A:THR345 4.5 26.6 1.0 O3P A:AMP102 4.6 33.6 0.8 CD2 A:HIS278 4.6 24.1 1.0 OD1 A:ASP392 4.7 24.8 1.0 CB A:THR345 4.7 28.0 1.0 NE2 A:HIS274 4.8 17.9 1.0 CA A:ASP275 4.9 23.7 1.0 CD A:GLU304 4.9 28.0 1.0 NE2 A:HIS278 5.0 21.2 1.0 CG A:GLU304 5.0 26.5 1.0

Magnesium binding site 2 out of 2 in the Catalytic Domain of Human Phosphodiesterase 4B in Complex with Amp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Catalytic Domain of Human Phosphodiesterase 4B in Complex with Amp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1002 b:25.2 occ:1.00 O B:HOH2007 2.0 31.2 1.0 O1P B:AMP101 2.1 43.9 0.8 O B:HOH2004 2.1 31.4 1.0 OD1 B:ASP275 2.1 22.8 1.0 O B:HOH2005 2.2 36.3 1.0 O B:HOH2003 2.2 29.9 1.0 CG B:ASP275 3.2 24.9 1.0 P B:AMP101 3.2 47.8 0.8 OD2 B:ASP275 3.5 22.0 1.0 O5' B:AMP101 3.6 55.6 0.8 ZN B:ZN1001 3.6 39.2 1.0 O2P B:AMP101 3.9 45.3 0.8 O B:HOH13 4.0 37.6 1.0 CD2 B:HIS274 4.1 23.2 1.0 OE2 B:GLU304 4.1 24.9 1.0 OG1 B:THR345 4.2 28.0 1.0 CE1 B:HIS307 4.2 22.7 1.0 O B:HIS274 4.2 26.5 1.0 OD1 B:ASP392 4.4 27.2 1.0 NE2 B:HIS274 4.5 11.9 1.0 O3P B:AMP101 4.5 49.9 0.8 CD2 B:HIS278 4.5 23.0 1.0 CB B:ASP275 4.5 24.3 1.0 ND1 B:HIS307 4.6 25.7 1.0 NE2 B:HIS278 4.6 21.4 1.0 CB B:THR345 4.6 26.9 1.0 O B:THR345 4.8 28.4 1.0 CA B:ASP275 4.9 24.5 1.0 C5' B:AMP101 4.9 63.5 0.8 NE2 B:HIS234 4.9 25.9 1.0 CG B:GLU304 4.9 23.5 1.0

K.Y.J.Zhang, G.L.Card, Y.Suzuki, D.R.Artis, D.Fong, S.Gillette, D.Hsieh, J.Neiman, B.L.West, C.Zhang, M.V.Milburn, S.-H.Kim, J.Schlessinger, G.Bollag. A Glutamine Switch Mechanism For Nucleotide Selectivity By Phosphodiesterases Mol.Cell V. 15 279 2004.
ISSN: ISSN 1097-2765
PubMed: 15260978
DOI: 10.1016/J.MOLCEL.2004.07.005