Magnesium in PDB 1tj5: X-Ray Structure of the Sucrose-Phosphatase (Spp) From Synechocystis Sp. PCC6803 in Complex with Sucrose and Phosphate

All present enzymatic activity of X-Ray Structure of the Sucrose-Phosphatase (Spp) From Synechocystis Sp. PCC6803 in Complex with Sucrose and Phosphate:
3.1.3.24;

The structure of X-Ray Structure of the Sucrose-Phosphatase (Spp) From Synechocystis Sp. PCC6803 in Complex with Sucrose and Phosphate, PDB code: 1tj5 was solved by S.Fieulaine, J.E.Lunn, F.Borel, J.-L.Ferrer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.20
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	68.750, 68.750, 268.380, 90.00, 90.00, 120.00
R / Rfree (%)	18.9 / 22

The binding sites of Magnesium atom in the X-Ray Structure of the Sucrose-Phosphatase (Spp) From Synechocystis Sp. PCC6803 in Complex with Sucrose and Phosphate (pdb code 1tj5). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the X-Ray Structure of the Sucrose-Phosphatase (Spp) From Synechocystis Sp. PCC6803 in Complex with Sucrose and Phosphate, PDB code: 1tj5:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the X-Ray Structure of the Sucrose-Phosphatase (Spp) From Synechocystis Sp. PCC6803 in Complex with Sucrose and Phosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of X-Ray Structure of the Sucrose-Phosphatase (Spp) From Synechocystis Sp. PCC6803 in Complex with Sucrose and Phosphate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg247 b:10.5 occ:0.70 OD2 A:ASP9 2.2 21.0 1.0 MG A:MG248 2.5 8.5 0.3 OD1 A:ASP186 2.5 26.6 1.0 OD2 A:ASP190 2.6 12.9 1.0 OG A:SER187 2.6 12.1 1.0 OD1 A:ASN189 2.6 14.2 1.0 O A:HOH463 2.7 27.2 1.0 O3 A:PO4246 3.2 28.2 0.5 O2 A:PO4246 3.3 28.3 0.5 CG A:ASP190 3.4 13.2 1.0 CG A:ASP9 3.4 19.1 1.0 CG A:ASN189 3.5 14.0 1.0 CB A:SER187 3.6 13.6 1.0 OD1 A:ASP190 3.6 11.2 1.0 P A:PO4246 3.7 26.2 0.5 CG A:ASP186 3.7 26.6 1.0 ND2 A:ASN189 3.7 10.9 1.0 O4 A:PO4246 3.8 26.3 0.5 N A:SER187 3.8 13.3 1.0 NZ A:LYS163 4.1 10.7 1.0 OD1 A:ASP9 4.2 22.9 1.0 CA A:SER187 4.2 13.8 1.0 OD2 A:ASP186 4.3 29.4 1.0 CB A:ASP9 4.4 15.4 1.0 O6' A:SUC245 4.4 39.8 1.0 N A:ASP186 4.5 6.9 1.0 C A:ASP186 4.6 8.3 1.0 CB A:ASP190 4.7 12.3 1.0 C A:SER187 4.7 14.1 1.0 O A:HOH384 4.7 43.3 1.0 O A:ASP11 4.8 13.5 1.0 CB A:ASN189 4.8 11.4 1.0 CB A:ASP186 4.8 21.2 1.0 N A:ASP190 4.9 11.2 1.0 O A:SER187 4.9 15.3 1.0 CA A:ASP186 4.9 8.2 1.0 N A:ASN189 5.0 12.9 1.0 C A:ASN189 5.0 12.8 1.0

Magnesium binding site 2 out of 2 in the X-Ray Structure of the Sucrose-Phosphatase (Spp) From Synechocystis Sp. PCC6803 in Complex with Sucrose and Phosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of X-Ray Structure of the Sucrose-Phosphatase (Spp) From Synechocystis Sp. PCC6803 in Complex with Sucrose and Phosphate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg248 b:8.5 occ:0.30 MG A:MG247 2.5 10.5 0.7 OD1 A:ASP186 2.5 26.6 1.0 O A:ASP11 2.6 13.5 1.0 O2 A:PO4246 2.6 28.3 0.5 OD2 A:ASP9 2.8 21.0 1.0 O6' A:SUC245 2.9 39.8 1.0 O A:HOH463 3.1 27.2 1.0 CG A:ASP186 3.3 26.6 1.0 OD2 A:ASP186 3.4 29.4 1.0 C6' A:SUC245 3.5 39.5 1.0 O3 A:PO4246 3.5 28.2 0.5 C A:ASP11 3.6 12.2 1.0 O A:HOH384 3.6 43.3 1.0 P A:PO4246 3.6 26.2 0.5 CG A:ASP9 3.7 19.1 1.0 ND2 A:ASN12 3.8 25.4 1.0 CB A:ASP11 4.1 20.1 1.0 OD1 A:ASP9 4.1 22.9 1.0 CB A:ASN12 4.2 21.3 1.0 CA A:ASP11 4.3 12.1 1.0 OG A:SER187 4.3 12.1 1.0 O4 A:PO4246 4.5 26.3 0.5 CG A:ASN12 4.5 24.6 1.0 OG1 A:THR13 4.5 18.5 1.0 N A:ASP11 4.6 8.6 1.0 OD2 A:ASP190 4.6 12.9 1.0 N A:ASN12 4.6 16.6 1.0 CB A:ASP186 4.7 21.2 1.0 O1 A:PO4246 4.7 28.1 0.5 CB A:ASP9 4.7 15.4 1.0 O A:HOH505 4.7 14.5 1.0 CB A:SER187 4.9 13.6 1.0 CA A:ASN12 4.9 16.3 1.0 C5' A:SUC245 4.9 41.0 1.0 OD1 A:ASN189 5.0 14.2 1.0

S.Fieulaine, J.E.Lunn, F.Borel, J.-L.Ferrer. The Structure of A Cyanobacterial Sucrose-Phosphatase Reveals the Sugar Tongs That Release Free Sucrose in the Cell. Plant Cell V. 17 2049 2005.
ISSN: ISSN 1040-4651
PubMed: 15937230
DOI: 10.1105/TPC.105.031229