Magnesium in PDB 1tk5: T7 Dna Polymerase Binary Complex with 8 Oxo Guanosine in the Templating Strand

All present enzymatic activity of T7 Dna Polymerase Binary Complex with 8 Oxo Guanosine in the Templating Strand:
2.7.7.7;

The structure of T7 Dna Polymerase Binary Complex with 8 Oxo Guanosine in the Templating Strand, PDB code: 1tk5 was solved by L.G.Brieba, B.F.Eichman, R.J.Kokoska, S.Doublie, T.A.Kunkel, T.Ellenberger, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.33 / 2.20
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	105.310, 213.265, 52.181, 90.00, 90.00, 90.00
R / Rfree (%)	21.4 / 24.6

The binding sites of Magnesium atom in the T7 Dna Polymerase Binary Complex with 8 Oxo Guanosine in the Templating Strand (pdb code 1tk5). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the T7 Dna Polymerase Binary Complex with 8 Oxo Guanosine in the Templating Strand, PDB code: 1tk5:

Magnesium binding site 1 out of 1 in the T7 Dna Polymerase Binary Complex with 8 Oxo Guanosine in the Templating Strand


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of T7 Dna Polymerase Binary Complex with 8 Oxo Guanosine in the Templating Strand within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg903 b:30.6 occ:1.00 OD1 A:ASP5 2.1 23.0 1.0 O A:HOH1005 2.1 45.5 1.0 O A:HOH1004 2.2 19.9 1.0 O A:HOH1003 2.2 30.3 1.0 O A:HOH1001 2.3 28.4 1.0 O A:HOH1002 2.3 23.8 1.0 CG A:ASP5 3.2 19.6 1.0 OD2 A:ASP5 3.6 23.4 1.0 O A:HOH1395 3.7 58.6 1.0 OD2 A:ASP65 4.0 19.3 1.0 O A:ILE6 4.1 18.4 1.0 O A:HOH1293 4.1 27.6 1.0 OD1 A:ASP65 4.2 18.6 1.0 O A:HOH1045 4.2 23.1 1.0 O A:HOH1044 4.3 24.6 1.0 O A:HOH1036 4.3 23.0 1.0 O A:HIS59 4.4 13.7 1.0 CB A:ASP5 4.5 15.5 1.0 CG A:ASP65 4.6 19.4 1.0 OD2 A:ASP174 4.8 38.1 1.0 N A:ILE6 4.8 16.5 1.0 CE A:MET107 5.0 30.1 1.0

L.G.Brieba, B.F.Eichman, R.J.Kokoska, S.Doublie, T.A.Kunkel, T.Ellenberger. Structural Basis For the Dual Coding Potential of 8-Oxoguanosine By A High-Fidelity Dna Polymerase. Embo J. V. 23 3452 2004.
ISSN: ISSN 0261-4189
PubMed: 15297882
DOI: 10.1038/SJ.EMBOJ.7600354