Atomistry » Magnesium » PDB 1w55-1wdd » 1w5o
Atomistry »
  Magnesium »
    PDB 1w55-1wdd »
      1w5o »

Magnesium in PDB 1w5o: Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C)

Enzymatic activity of Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C)

All present enzymatic activity of Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C):
4.2.1.24;

Protein crystallography data

The structure of Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C), PDB code: 1w5o was solved by F.Frere, H.Reents, W.-D.Schubert, D.W.Heinz, D.Jahn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 91.29 / 1.85
Space group P 4 21 2
Cell size a, b, c (Å), α, β, γ (°) 126.991, 126.991, 85.369, 90.00, 90.00, 90.00
R / Rfree (%) 13.1 / 17.6

Other elements in 1w5o:

The structure of Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C) also contains other interesting chemical elements:

Potassium (K) 2 atoms
Zinc (Zn) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C) (pdb code 1w5o). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C), PDB code: 1w5o:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 1w5o

Go back to Magnesium Binding Sites List in 1w5o
Magnesium binding site 1 out of 2 in the Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1338

b:16.2
occ:1.00
 OE1 A:GLU245 2.0 16.1 1.0
O A:HOH2208 2.0 16.5 1.0
O A:HOH2261 2.1 14.2 1.0
O A:HOH2258 2.1 16.3 1.0
O A:HOH2207 2.1 15.7 1.0
O A:HOH2260 2.2 15.4 1.0
CD A:GLU245 3.0 15.3 1.0
OE2 A:GLU245 3.4 16.6 1.0
NH1 A:ARG181 3.7 16.2 1.0
OD1 A:ASP249 3.8 14.7 1.0
O A:HOH2206 4.1 16.4 1.0
O A:HOH2264 4.1 16.9 1.0
OD2 A:ASP249 4.2 14.7 1.0
O A:HOH2263 4.2 17.5 1.0
O A:MET178 4.3 17.7 1.0
CG A:GLU245 4.3 12.9 1.0
CG A:ASP249 4.4 12.1 1.0
O A:SER203 4.5 17.2 1.0
OD1 A:ASP179 4.5 17.8 1.0
CB A:GLU245 4.5 11.9 1.0
O A:GLU245 4.5 14.6 1.0
CA A:GLU245 4.6 16.5 1.0
CA A:ASP179 4.7 13.6 1.0
O B:HOH2017 4.8 21.8 1.0
SD A:MET177 4.8 16.8 1.0
CE A:MET177 4.9 17.8 1.0

Magnesium binding site 2 out of 2 in 1w5o

Go back to Magnesium Binding Sites List in 1w5o
Magnesium binding site 2 out of 2 in the Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Stepwise Introduction of Zinc Binding Site Into Porphobilinogen Synthase of Pseudomonas Aeruginosa (Mutations A129C, D131C and D139C) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg1338

b:20.0
occ:1.00
 O B:HOH2291 2.0 19.0 1.0
OE1 B:GLU245 2.0 16.9 1.0
O B:HOH2244 2.0 19.2 1.0
O B:HOH2245 2.1 21.6 1.0
O B:HOH2289 2.1 18.2 1.0
O B:HOH2290 2.2 18.2 1.0
CD B:GLU245 3.0 18.1 1.0
OE2 B:GLU245 3.4 17.9 1.0
NH1 B:ARG181 3.8 22.0 1.0
OD1 B:ASP249 3.8 17.9 1.0
O B:HOH2243 4.1 17.9 1.0
O B:HOH2293 4.1 23.2 1.0
O B:HOH2294 4.2 21.5 1.0
OD2 B:ASP249 4.3 15.8 1.0
O B:MET178 4.3 21.1 1.0
CG B:GLU245 4.3 15.6 1.0
CG B:ASP249 4.4 17.1 1.0
O B:SER203 4.5 18.0 1.0
CB B:GLU245 4.5 15.0 1.0
OD1 B:ASP179 4.6 20.6 1.0
O B:GLU245 4.6 15.7 1.0
O A:HOH2013 4.7 25.3 1.0
CA B:GLU245 4.7 15.9 1.0
CA B:ASP179 4.7 20.7 1.0
SD B:MET177 4.9 20.8 1.0

Reference:

F.Frere, H.Reents, W.-D.Schubert, D.W.Heinz, D.Jahn. Tracking the Evolution of Porphobilinogen Synthase Metal Dependence in Vitro J.Mol.Biol. V. 345 1059 2005.
ISSN: ISSN 0022-2836
PubMed: 15644204
DOI: 10.1016/J.JMB.2004.10.053
Page generated: Mon Dec 14 07:01:27 2020

Last articles

Zn in 7VD8
Zn in 7V1R
Zn in 7V1Q
Zn in 7VPF
Zn in 7T85
Zn in 7T5F
Zn in 7NF9
Zn in 7M4M
Zn in 7M4O
Zn in 7M4N
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy