Atomistry » Magnesium » PDB 1xfy-1xon » 1xfz
Atomistry »
  Magnesium »
    PDB 1xfy-1xon »
      1xfz »

Magnesium in PDB 1xfz: Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride

Enzymatic activity of Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride

All present enzymatic activity of Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride:
4.6.1.1;

Protein crystallography data

The structure of Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride, PDB code: 1xfz was solved by Y.Shen, N.L.Zhukovskaya, Q.Guo, J.Florian, W.J.Tang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.99 / 3.25
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 183.258, 316.475, 140.801, 90.00, 89.91, 90.00
R / Rfree (%) 25.8 / 27

Other elements in 1xfz:

The structure of Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride also contains other interesting chemical elements:

Calcium (Ca) 18 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride (pdb code 1xfz). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride, PDB code: 1xfz:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6;

Magnesium binding site 1 out of 6 in 1xfz

Go back to Magnesium Binding Sites List in 1xfz
Magnesium binding site 1 out of 6 in the Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg901

b:26.2
occ:1.00
 NE2 A:HIS577 2.2 49.2 1.0
OD2 A:ASP493 2.3 48.9 1.0
OD1 A:ASP491 2.6 62.0 1.0
OD2 A:ASP491 2.7 60.7 1.0
CE1 A:HIS577 3.0 49.4 1.0
CG A:ASP491 3.0 60.9 1.0
OD1 A:ASP493 3.0 49.5 1.0
CG A:ASP493 3.0 49.3 1.0
CD2 A:HIS577 3.3 48.8 1.0
O A:LYS346 4.2 54.7 1.0
ND1 A:HIS577 4.2 49.0 1.0
CG A:HIS577 4.4 48.2 1.0
CB A:ASP493 4.5 48.4 1.0
CB A:ASP491 4.5 59.1 1.0
O A:GLY347 5.0 57.4 1.0

Magnesium binding site 2 out of 6 in 1xfz

Go back to Magnesium Binding Sites List in 1xfz
Magnesium binding site 2 out of 6 in the Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg902

b:28.7
occ:1.00
 NE2 B:HIS577 2.1 48.6 1.0
OD2 B:ASP493 2.3 48.5 1.0
OD1 B:ASP491 2.4 62.4 1.0
OD2 B:ASP491 2.7 60.7 1.0
CG B:ASP491 2.9 61.0 1.0
CE1 B:HIS577 2.9 48.7 1.0
CG B:ASP493 3.1 49.2 1.0
OD1 B:ASP493 3.1 49.5 1.0
CD2 B:HIS577 3.3 49.1 1.0
O B:LYS346 4.0 55.4 1.0
ND1 B:HIS577 4.1 48.8 1.0
CG B:HIS577 4.3 48.4 1.0
CB B:ASP491 4.4 58.9 1.0
CB B:ASP493 4.5 48.0 1.0
O B:GLY347 5.0 57.0 1.0
CA B:ASP493 5.0 50.0 1.0

Magnesium binding site 3 out of 6 in 1xfz

Go back to Magnesium Binding Sites List in 1xfz
Magnesium binding site 3 out of 6 in the Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg903

b:27.6
occ:1.00
 NE2 C:HIS577 2.0 48.6 1.0
OD2 C:ASP493 2.2 49.5 1.0
OD1 C:ASP491 2.3 61.7 1.0
OD2 C:ASP491 2.7 59.6 1.0
CE1 C:HIS577 2.8 48.6 1.0
CG C:ASP491 2.9 60.2 1.0
CG C:ASP493 3.0 50.0 1.0
OD1 C:ASP493 3.1 49.9 1.0
CD2 C:HIS577 3.1 48.0 1.0
ND1 C:HIS577 4.0 48.0 1.0
O C:LYS346 4.1 55.7 1.0
CG C:HIS577 4.2 47.5 1.0
CB C:ASP491 4.4 58.8 1.0
CB C:ASP493 4.4 48.8 1.0
CA C:ASP493 4.9 50.4 1.0

Magnesium binding site 4 out of 6 in 1xfz

Go back to Magnesium Binding Sites List in 1xfz
Magnesium binding site 4 out of 6 in the Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg904

b:29.1
occ:1.00
 NE2 D:HIS577 2.2 49.1 1.0
OD2 D:ASP493 2.4 49.5 1.0
OD1 D:ASP491 2.5 62.6 1.0
OD2 D:ASP491 2.7 61.0 1.0
CG D:ASP491 3.0 61.1 1.0
CE1 D:HIS577 3.0 49.2 1.0
OD1 D:ASP493 3.1 51.0 1.0
CG D:ASP493 3.1 50.1 1.0
CD2 D:HIS577 3.3 49.1 1.0
O D:LYS346 4.0 55.9 1.0
ND1 D:HIS577 4.2 48.8 1.0
CG D:HIS577 4.3 48.2 1.0
CB D:ASP491 4.5 58.9 1.0
CB D:ASP493 4.6 48.7 1.0
O D:GLY347 4.9 58.2 1.0

Magnesium binding site 5 out of 6 in 1xfz

Go back to Magnesium Binding Sites List in 1xfz
Magnesium binding site 5 out of 6 in the Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Mg905

b:23.2
occ:1.00
 NE2 E:HIS577 2.2 48.9 1.0
OD2 E:ASP493 2.3 49.3 1.0
OD1 E:ASP491 2.5 61.5 1.0
OD2 E:ASP491 2.7 60.6 1.0
CG E:ASP491 2.9 60.5 1.0
CE1 E:HIS577 3.0 49.0 1.0
CG E:ASP493 3.1 49.9 1.0
OD1 E:ASP493 3.1 50.2 1.0
CD2 E:HIS577 3.3 48.8 1.0
O E:LYS346 4.1 55.5 1.0
ND1 E:HIS577 4.2 49.2 1.0
CG E:HIS577 4.3 48.1 1.0
CB E:ASP491 4.4 59.2 1.0
CB E:ASP493 4.5 48.7 1.0
O E:GLY347 4.9 57.3 1.0

Magnesium binding site 6 out of 6 in 1xfz

Go back to Magnesium Binding Sites List in 1xfz
Magnesium binding site 6 out of 6 in the Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Crystal Structure of Anthrax Edema Factor (Ef) in Complex with Calmodulin in the Presence of 1 Millimolar Exogenously Added Calcium Chloride within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Mg906

b:25.2
occ:1.00
 NE2 F:HIS577 2.1 49.1 1.0
OD2 F:ASP493 2.4 50.1 1.0
OD1 F:ASP491 2.5 62.2 1.0
OD2 F:ASP491 2.8 61.1 1.0
CE1 F:HIS577 2.9 49.4 1.0
CG F:ASP491 3.0 61.3 1.0
CG F:ASP493 3.1 50.7 1.0
OD1 F:ASP493 3.1 50.6 1.0
CD2 F:HIS577 3.2 48.9 1.0
O F:LYS346 4.1 55.3 1.0
ND1 F:HIS577 4.1 49.3 1.0
CG F:HIS577 4.3 48.6 1.0
CB F:ASP491 4.5 59.7 1.0
CB F:ASP493 4.6 49.3 1.0
O F:GLY347 4.9 56.8 1.0

Reference:

Y.Shen, N.L.Zhukovskaya, Q.Guo, J.Florian, W.J.Tang. Calcium-Independent Calmodulin Binding and Two-Metal-Ion Catalytic Mechanism of Anthrax Edema Factor. Embo J. V. 24 929 2005.
ISSN: ISSN 0261-4189
PubMed: 15719022
DOI: 10.1038/SJ.EMBOJ.7600574
Page generated: Tue Aug 13 17:39:50 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy