Magnesium in PDB 1xss: Semi-Rational Engineering of A Green-Emitting Coral Fluorescent Protein Into An Efficient Highlighter.

The structure of Semi-Rational Engineering of A Green-Emitting Coral Fluorescent Protein Into An Efficient Highlighter., PDB code: 1xss was solved by H.Tsutsui, S.Karasawa, H.Shimizu, N.Nukina, A.Miyawaki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.50 / 1.60
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	96.740, 119.080, 49.290, 90.00, 120.72, 90.00
R / Rfree (%)	19.7 / 22.9

The structure of Semi-Rational Engineering of A Green-Emitting Coral Fluorescent Protein Into An Efficient Highlighter. also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

The binding sites of Magnesium atom in the Semi-Rational Engineering of A Green-Emitting Coral Fluorescent Protein Into An Efficient Highlighter. (pdb code 1xss). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Semi-Rational Engineering of A Green-Emitting Coral Fluorescent Protein Into An Efficient Highlighter., PDB code: 1xss:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Semi-Rational Engineering of A Green-Emitting Coral Fluorescent Protein Into An Efficient Highlighter.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Semi-Rational Engineering of A Green-Emitting Coral Fluorescent Protein Into An Efficient Highlighter. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1300 b:20.8 occ:1.00 O A:HOH1364 2.5 16.9 1.0 O A:HOH1356 2.5 15.3 1.0 OH A:TYR207 2.5 22.9 1.0 O A:HOH1448 2.5 27.1 1.0 O A:HOH1442 2.6 25.4 1.0 CZ A:TYR207 3.7 18.3 1.0 O A:HOH1550 3.9 35.1 1.0 O A:VAL140 3.9 11.0 1.0 NE2 A:HIS203 4.0 15.4 1.0 CE2 A:TYR207 4.0 12.8 1.0 O A:HOH1317 4.0 10.3 1.0 O A:HOH1432 4.2 25.3 1.0 O A:HOH1565 4.2 41.5 1.0 O A:HOH1422 4.3 25.2 1.0 O A:HOH1373 4.3 18.2 1.0 O A:THR139 4.3 12.5 1.0 O A:HOH1410 4.4 21.8 1.0 CD2 A:HIS203 4.4 15.7 1.0 CE1 A:HIS203 4.7 17.6 1.0 O A:HOH1332 4.8 11.7 1.0 C A:VAL140 4.8 10.5 1.0 CA A:VAL140 4.9 10.9 1.0 CE1 A:TYR207 4.9 19.4 1.0

Magnesium binding site 2 out of 2 in the Semi-Rational Engineering of A Green-Emitting Coral Fluorescent Protein Into An Efficient Highlighter.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Semi-Rational Engineering of A Green-Emitting Coral Fluorescent Protein Into An Efficient Highlighter. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2300 b:23.6 occ:1.00 O B:HOH2353 2.4 15.0 1.0 OH B:TYR207 2.4 22.3 1.0 O B:HOH2389 2.5 21.1 1.0 O B:HOH2432 2.5 26.4 1.0 O B:HOH2348 2.6 14.4 1.0 O B:HOH2459 2.6 32.5 1.0 CZ B:TYR207 3.6 16.3 1.0 NE2 B:HIS203 3.9 16.4 1.0 CE2 B:TYR207 4.0 13.4 1.0 O B:VAL140 4.0 10.5 1.0 O B:HOH2324 4.2 11.0 1.0 O B:HOH2428 4.2 24.8 1.0 O B:THR139 4.2 11.5 1.0 O B:HOH2530 4.3 37.7 1.0 O B:HOH2403 4.3 22.3 1.0 O B:HOH2443 4.3 27.5 1.0 CD2 B:HIS203 4.4 16.1 1.0 O B:HOH2378 4.4 19.3 1.0 CE1 B:HIS203 4.6 17.7 1.0 CE1 B:TYR207 4.8 19.1 1.0 O B:HOH2346 4.8 13.7 1.0 C B:VAL140 4.8 10.3 1.0 CA B:VAL140 4.9 8.4 1.0

H.Tsutsui, S.Karasawa, H.Shimizu, N.Nukina, A.Miyawaki. Semi-Rational Engineering of A Coral Fluorescent Protein Into An Efficient Highlighter Embo Rep. V. 6 233 2005.
ISSN: ISSN 1469-221X
PubMed: 15731765
DOI: 10.1038/SJ.EMBOR.7400361