Magnesium in PDB 1yjf: Cyclized Post-Translational Product For S65A Y66S (Gfphal) Green Fluorescent Protein Variant

The structure of Cyclized Post-Translational Product For S65A Y66S (Gfphal) Green Fluorescent Protein Variant, PDB code: 1yjf was solved by D.P.Barondeau, C.J.Kassmann, J.A.Tainer, E.D.Getzoff, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.35
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.971, 62.636, 71.532, 90.00, 90.00, 90.00
R / Rfree (%)	14.5 / 20.5

The binding sites of Magnesium atom in the Cyclized Post-Translational Product For S65A Y66S (Gfphal) Green Fluorescent Protein Variant (pdb code 1yjf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cyclized Post-Translational Product For S65A Y66S (Gfphal) Green Fluorescent Protein Variant, PDB code: 1yjf:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cyclized Post-Translational Product For S65A Y66S (Gfphal) Green Fluorescent Protein Variant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cyclized Post-Translational Product For S65A Y66S (Gfphal) Green Fluorescent Protein Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:15.0 occ:1.00 O A:HOH1416 2.0 15.4 1.0 O A:HOH1418 2.0 21.6 1.0 O A:HOH1421 2.0 16.0 1.0 O A:HOH1417 2.0 20.4 1.0 O A:HOH1420 2.1 17.5 1.0 O A:HOH1419 2.1 15.9 1.0 OD1 A:ASP197 4.0 21.0 1.0 OD2 A:ASP197 4.0 19.1 1.0 O A:HOH1264 4.1 36.3 1.0 O A:HOH1119 4.3 27.4 1.0 CG A:ASP197 4.5 15.9 1.0 O A:HOH1253 4.5 43.5 1.0 O A:HOH1357 4.9 36.8 1.0

Magnesium binding site 2 out of 2 in the Cyclized Post-Translational Product For S65A Y66S (Gfphal) Green Fluorescent Protein Variant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cyclized Post-Translational Product For S65A Y66S (Gfphal) Green Fluorescent Protein Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:27.4 occ:1.00 O A:HOH1427 2.0 37.2 1.0 O A:HOH1424 2.0 25.4 1.0 O A:HOH1423 2.0 31.8 1.0 O A:HOH1422 2.0 37.3 1.0 O A:HOH1425 2.0 29.6 1.0 O A:HOH1426 2.0 46.1 1.0 O A:HOH1401 4.0 50.0 1.0 O A:HOH1165 4.0 29.8 1.0 O A:HOH1203 4.3 38.0 1.0 O A:HOH1066 4.3 21.0 1.0 O A:HOH1170 4.4 26.1 1.0 OG1 A:THR38 4.5 17.0 1.0 CE A:LYS79 4.6 34.3 1.0 CG2 A:THR38 4.9 14.8 1.0

D.P.Barondeau, C.J.Kassmann, J.A.Tainer, E.D.Getzoff. Understanding Gfp Chromophore Biosynthesis: Controlling Backbone Cyclization and Modifying Post-Translational Chemistry. Biochemistry V. 44 1960 2005.
ISSN: ISSN 0006-2960
PubMed: 15697221
DOI: 10.1021/BI0479205