Magnesium in PDB 1z2c: Crystal Structure of MDIA1 Gbd-FH3 in Complex with Rhoc-Gmppnp

The structure of Crystal Structure of MDIA1 Gbd-FH3 in Complex with Rhoc-Gmppnp, PDB code: 1z2c was solved by R.Rose, M.Weyand, M.Lammers, T.Ishizaki, M.R.Ahmadian, A.Wittinghofer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 3.00
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	148.640, 85.350, 123.380, 90.00, 90.00, 90.00
R / Rfree (%)	21.1 / 28.5

The binding sites of Magnesium atom in the Crystal Structure of MDIA1 Gbd-FH3 in Complex with Rhoc-Gmppnp (pdb code 1z2c). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure of MDIA1 Gbd-FH3 in Complex with Rhoc-Gmppnp, PDB code: 1z2c:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystal Structure of MDIA1 Gbd-FH3 in Complex with Rhoc-Gmppnp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of MDIA1 Gbd-FH3 in Complex with Rhoc-Gmppnp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg194 b:31.7 occ:1.00 OG1 A:THR19 2.0 45.4 1.0 O2B A:GNP195 2.1 46.1 1.0 OG1 A:THR37 2.2 41.6 1.0 O2G A:GNP195 2.3 44.4 1.0 N3B A:GNP195 3.0 46.6 1.0 CB A:THR19 3.1 47.5 1.0 PB A:GNP195 3.1 46.1 1.0 PG A:GNP195 3.2 45.4 1.0 CB A:THR37 3.3 44.4 1.0 N A:THR37 3.5 46.3 1.0 O2A A:GNP195 3.5 53.3 1.0 CG2 A:THR37 3.6 47.7 1.0 N A:THR19 4.0 43.6 1.0 CA A:THR37 4.0 45.9 1.0 CG2 A:THR19 4.1 49.8 1.0 O1G A:GNP195 4.1 43.5 1.0 CA A:THR19 4.1 46.7 1.0 O3A A:GNP195 4.2 49.6 1.0 OD1 A:ASP59 4.2 38.0 1.0 O1B A:GNP195 4.3 47.0 1.0 OD2 A:ASP59 4.3 41.6 1.0 PA A:GNP195 4.3 54.3 1.0 O A:VAL35 4.5 56.8 1.0 C A:PRO36 4.5 48.9 1.0 O3G A:GNP195 4.5 43.0 1.0 CG A:ASP59 4.7 38.4 1.0 CA A:PRO36 4.7 50.6 1.0 O1A A:GNP195 4.8 52.8 1.0 O A:THR60 4.8 32.4 1.0

Magnesium binding site 2 out of 3 in the Crystal Structure of MDIA1 Gbd-FH3 in Complex with Rhoc-Gmppnp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of MDIA1 Gbd-FH3 in Complex with Rhoc-Gmppnp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg5 b:66.6 occ:1.00 OD2 B:ASP366 4.0 35.6 1.0 OE1 B:GLU362 4.1 50.1 1.0 OD1 B:ASP366 4.5 41.0 1.0 CG B:ASP366 4.7 37.8 1.0

Magnesium binding site 3 out of 3 in the Crystal Structure of MDIA1 Gbd-FH3 in Complex with Rhoc-Gmppnp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of MDIA1 Gbd-FH3 in Complex with Rhoc-Gmppnp within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg194 b:56.6 occ:1.00 O2G C:GNP195 1.8 50.6 1.0 OG1 C:THR19 1.8 52.0 1.0 O2B C:GNP195 2.0 54.5 1.0 OG1 C:THR37 2.5 48.9 1.0 CB C:THR19 3.0 53.2 1.0 PG C:GNP195 3.0 50.4 1.0 PB C:GNP195 3.1 54.8 1.0 N3B C:GNP195 3.1 52.5 1.0 O2A C:GNP195 3.2 53.8 1.0 CB C:THR37 3.4 51.0 1.0 N C:THR37 3.5 54.3 1.0 CG2 C:THR37 3.5 53.5 1.0 N C:THR19 3.9 49.3 1.0 CG2 C:THR19 3.9 54.7 1.0 CA C:THR19 4.0 51.5 1.0 O3A C:GNP195 4.1 55.1 1.0 O1G C:GNP195 4.1 49.5 1.0 CA C:THR37 4.1 53.5 1.0 O3G C:GNP195 4.1 49.7 1.0 PA C:GNP195 4.2 55.6 1.0 O1B C:GNP195 4.3 54.6 1.0 OD2 C:ASP59 4.5 49.4 1.0 C C:PRO36 4.5 57.2 1.0 O C:VAL35 4.5 62.2 1.0 CA C:PRO36 4.6 59.0 1.0 OD1 C:ASP59 4.8 42.1 1.0 CE1 C:TYR34 4.9 65.3 1.0

R.Rose, M.Weyand, M.Lammers, T.Ishizaki, M.R.Ahmadian, A.Wittinghofer. Structural and Mechanistic Insights Into the Interaction Between Rho and Mammalian Dia. Nature V. 435 513 2005.
ISSN: ISSN 0028-0836
PubMed: 15864301
DOI: 10.1038/NATURE03604