Magnesium in PDB 1zs9: Crystal Structure of Human Enolase-Phosphatase E1

The structure of Crystal Structure of Human Enolase-Phosphatase E1, PDB code: 1zs9 was solved by H.Wang, H.Pang, M.Bartlam, Z.Rao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	54.017, 57.546, 87.318, 90.00, 90.00, 90.00
R / Rfree (%)	20.8 / 24.1

The binding sites of Magnesium atom in the Crystal Structure of Human Enolase-Phosphatase E1 (pdb code 1zs9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure of Human Enolase-Phosphatase E1, PDB code: 1zs9:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystal Structure of Human Enolase-Phosphatase E1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Human Enolase-Phosphatase E1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1257 b:12.1 occ:1.00 O A:HOH1261 2.0 15.7 1.0 O A:HOH1262 2.0 13.3 1.0 OD1 A:ASP212 2.0 14.9 1.0 OD2 A:ASP16 2.0 15.1 1.0 O A:HOH1260 2.1 12.9 1.0 O A:GLU18 2.1 12.5 1.0 CG A:ASP16 3.0 16.4 1.0 CG A:ASP212 3.1 15.9 1.0 C A:GLU18 3.3 13.5 1.0 OD1 A:ASP16 3.3 13.7 1.0 OD2 A:ASP212 3.5 16.5 1.0 OG1 A:THR20 4.0 13.3 1.0 CA A:GLU18 4.1 12.9 1.0 CG2 A:VAL213 4.2 18.5 1.0 CB A:GLU18 4.3 12.3 1.0 OG1 A:THR211 4.3 16.5 1.0 N A:GLY19 4.3 12.7 1.0 N A:GLU18 4.3 12.5 1.0 CB A:ASP16 4.3 13.8 1.0 N A:ASP212 4.4 15.9 1.0 CB A:ASP212 4.4 16.8 1.0 CA A:GLY19 4.5 13.3 1.0 OE2 A:GLU216 4.6 17.6 1.0 N A:VAL213 4.7 15.4 1.0 C A:GLY19 4.7 14.0 1.0 N A:THR20 4.8 13.3 1.0 CA A:ASP212 4.9 15.1 1.0 OE1 A:GLU216 4.9 16.1 1.0 C A:ILE17 4.9 13.2 1.0 CG A:GLU18 5.0 17.0 1.0 CB A:VAL213 5.0 17.3 1.0

Magnesium binding site 2 out of 3 in the Crystal Structure of Human Enolase-Phosphatase E1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Human Enolase-Phosphatase E1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1258 b:14.5 occ:1.00 OD1 A:ASP198 2.0 13.4 1.0 O A:HOH1263 2.1 12.8 1.0 O A:HOH1264 2.2 13.0 1.0 O A:HOH1266 2.2 12.4 1.0 O A:HOH1267 2.2 14.1 1.0 O A:HOH1265 2.2 14.3 1.0 CG A:ASP198 3.0 14.1 1.0 OD2 A:ASP198 3.4 15.7 1.0 O A:HOH1288 4.2 21.6 1.0 O A:HOH1324 4.2 19.2 1.0 O A:ARG194 4.2 13.6 1.0 CB A:ASP198 4.3 14.7 1.0 CA A:ASP198 4.5 14.8 1.0 N A:ASP198 4.6 14.7 1.0 O A:CYS202 4.8 16.6 1.0

Magnesium binding site 3 out of 3 in the Crystal Structure of Human Enolase-Phosphatase E1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Human Enolase-Phosphatase E1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1259 b:26.5 occ:1.00 O A:HOH1271 2.0 20.6 1.0 O A:HOH1273 2.2 19.4 1.0 O A:HOH1270 2.2 19.9 1.0 O A:HOH1268 2.2 23.8 1.0 O A:HOH1269 2.3 33.9 1.0 O A:HOH1272 2.3 28.2 1.0 OE2 A:GLU9 4.1 22.0 1.0 OD1 A:ASP224 4.2 19.2 1.0 O A:HOH1363 4.3 22.4 1.0 CB A:ASN205 4.3 18.9 1.0 OD2 A:ASP224 4.4 18.1 1.0 O A:HOH1515 4.4 44.0 1.0 OE1 A:GLU9 4.5 21.9 1.0 CG A:ASP224 4.7 18.5 1.0 CD A:GLU9 4.7 22.2 1.0 O A:HOH1371 4.8 31.3 1.0 CG A:ASN205 4.9 21.0 1.0

H.Wang, H.Pang, M.Bartlam, Z.Rao. Crystal Structure of Human E1 Enzyme and Its Complex with A Substrate Analog Reveals the Mechanism of Its Phosphatase/Enolase J.Mol.Biol. V. 348 917 2005.
ISSN: ISSN 0022-2836
PubMed: 15843022
DOI: 10.1016/J.JMB.2005.01.072