Magnesium in PDB 1zy5: Crystal Structure of EIF2ALPHA Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with Amppnp.

All present enzymatic activity of Crystal Structure of EIF2ALPHA Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with Amppnp.:
2.7.1.37;

The structure of Crystal Structure of EIF2ALPHA Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with Amppnp., PDB code: 1zy5 was solved by A.K.Padyana, H.Qiu, A.Roll-Mecak, A.G.Hinnebusch, S.K.Burley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.58 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	52.610, 78.919, 146.299, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Magnesium atom in the Crystal Structure of EIF2ALPHA Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with Amppnp. (pdb code 1zy5). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of EIF2ALPHA Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with Amppnp., PDB code: 1zy5:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of EIF2ALPHA Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with Amppnp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of EIF2ALPHA Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with Amppnp. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg305 b:98.3 occ:1.00 O1A A:ANP303 2.0 64.9 1.0 O2G A:ANP303 2.2 94.9 1.0 O A:HOH205 2.3 73.4 1.0 O3G A:ANP303 2.8 92.9 1.0 PG A:ANP303 3.0 97.1 1.0 NZ A:LYS628 3.3 52.1 1.0 PA A:ANP303 3.4 72.9 1.0 O2A A:ANP303 3.9 63.1 1.0 N3B A:ANP303 4.0 80.0 1.0 O A:HOH114 4.0 41.0 1.0 O1G A:ANP303 4.1 90.3 1.0 OD2 A:ASP853 4.2 37.9 1.0 O1B A:ANP303 4.2 78.1 1.0 O3A A:ANP303 4.3 63.1 1.0 O5' A:ANP303 4.4 59.0 1.0 PB A:ANP303 4.4 71.8 1.0 CE A:LYS628 4.7 44.9 1.0 O A:HOH138 4.7 56.1 1.0 O A:HOH90 4.7 50.1 1.0 C5' A:ANP303 4.8 48.7 1.0 CG A:ASP853 4.9 48.2 1.0 OD1 A:ASP853 4.9 60.1 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of EIF2ALPHA Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with Amppnp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of EIF2ALPHA Protein Kinase GCN2: R794G Hyperactivating Mutant Complexed with Amppnp. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg306 b:94.7 occ:1.00 O1B B:ANP304 2.0 89.1 1.0 O2G B:ANP304 2.0 0.4 1.0 PB B:ANP304 3.3 0.7 1.0 PG B:ANP304 3.3 0.7 1.0 O1A B:ANP304 3.3 86.3 1.0 N3B B:ANP304 3.7 0.6 1.0 CZ B:PHE610 3.8 0.1 1.0 O3A B:ANP304 4.0 97.5 1.0 PA B:ANP304 4.1 84.5 1.0 O B:HOH201 4.3 70.7 1.0 O3G B:ANP304 4.3 0.1 1.0 O1G B:ANP304 4.3 0.1 1.0 O2B B:ANP304 4.4 1.0 1.0 CE2 B:PHE610 4.4 0.0 1.0 O2A B:ANP304 4.4 87.3 1.0 CE1 B:PHE610 4.7 0.6 1.0 CA B:GLY608 4.7 71.4 1.0 O B:GLN607 4.7 73.4 1.0

A.K.Padyana, H.Qiu, A.Roll-Mecak, A.G.Hinnebusch, S.K.Burley. Structural Basis For Autoinhibition and Mutational Activation of Eukaryotic Initiation Factor 2{Alpha} Protein Kinase GCN2 J.Biol.Chem. V. 280 29289 2005.
ISSN: ISSN 0021-9258
PubMed: 15964839
DOI: 10.1074/JBC.M504096200