Magnesium in PDB 1zyd: Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp.

All present enzymatic activity of Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp.:
2.7.1.37;

The structure of Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp., PDB code: 1zyd was solved by A.K.Padyana, H.Qiu, A.Roll-Mecak, A.G.Hinnebusch, S.K.Burley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.59 / 2.75
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	82.110, 175.210, 47.570, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Magnesium atom in the Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp. (pdb code 1zyd). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp., PDB code: 1zyd:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg305 b:27.7 occ:1.00 O1A A:ATP303 2.1 47.6 1.0 OD2 A:ASP853 2.5 34.7 1.0 OD1 A:ASP853 2.7 37.3 1.0 O2G A:ATP303 2.8 52.0 1.0 O3G A:ATP303 2.9 60.1 1.0 CG A:ASP853 2.9 40.4 1.0 O3B A:ATP303 3.0 54.9 1.0 PG A:ATP303 3.0 49.3 1.0 PA A:ATP303 3.4 46.2 1.0 OD1 A:ASP835 3.9 45.2 1.0 O A:ASP853 4.1 32.7 1.0 O3A A:ATP303 4.1 46.6 1.0 O2A A:ATP303 4.1 45.0 1.0 MG A:MG306 4.1 26.6 1.0 PB A:ATP303 4.3 48.5 1.0 CB A:ASP853 4.3 31.5 1.0 NZ A:LYS628 4.5 40.1 1.0 O5' A:ATP303 4.5 51.0 1.0 O1G A:ATP303 4.5 52.5 1.0 OD1 A:ASN840 4.8 37.4 1.0 C A:ASP853 4.8 32.0 1.0 ND2 A:ASN840 4.9 38.6 1.0 O A:HOH64 4.9 35.8 1.0 O1B A:ATP303 4.9 63.2 1.0

Magnesium binding site 2 out of 4 in the Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg306 b:26.6 occ:1.00 OD1 A:ASN840 2.2 37.4 1.0 O3B A:ATP303 2.4 54.9 1.0 OD2 A:ASP853 2.5 34.7 1.0 CG A:ASN840 3.3 33.4 1.0 PB A:ATP303 3.3 48.5 1.0 O2B A:ATP303 3.3 52.3 1.0 O3G A:ATP303 3.3 60.1 1.0 PG A:ATP303 3.3 49.3 1.0 O3A A:ATP303 3.6 46.6 1.0 CG A:ASP853 3.6 40.4 1.0 ND2 A:ASN840 3.7 38.6 1.0 O1G A:ATP303 4.0 52.5 1.0 O A:MET839 4.1 35.8 1.0 O2' A:ATP303 4.1 40.7 1.0 MG A:MG305 4.1 27.7 1.0 O1A A:ATP303 4.2 47.6 1.0 C2' A:ATP303 4.2 37.9 1.0 CB A:ASP853 4.3 31.5 1.0 PA A:ATP303 4.5 46.2 1.0 CB A:ASN840 4.6 27.5 1.0 OD1 A:ASP853 4.6 37.3 1.0 O2G A:ATP303 4.6 52.0 1.0 C A:MET839 4.6 32.6 1.0 C3' A:ATP303 4.7 35.9 1.0 CA A:ASN840 4.7 27.0 1.0 O1B A:ATP303 4.8 63.2 1.0 N A:ASN840 5.0 30.8 1.0

Magnesium binding site 3 out of 4 in the Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg307 b:22.6 occ:1.00 O1A B:ATP304 2.1 47.4 1.0 OD2 B:ASP853 2.3 33.9 1.0 OD1 B:ASP853 2.7 48.0 1.0 CG B:ASP853 2.8 36.6 1.0 O3G B:ATP304 2.8 62.5 1.0 O3B B:ATP304 3.0 56.5 1.0 O2G B:ATP304 3.1 59.8 1.0 PG B:ATP304 3.2 52.4 1.0 PA B:ATP304 3.5 49.2 1.0 MG B:MG308 4.1 42.2 1.0 O3A B:ATP304 4.1 52.1 1.0 O2A B:ATP304 4.2 47.9 1.0 NZ B:LYS628 4.3 54.8 1.0 CB B:ASP853 4.3 37.0 1.0 PB B:ATP304 4.3 55.5 1.0 O B:ASP853 4.4 36.6 1.0 OD2 B:ASP835 4.6 50.7 1.0 O5' B:ATP304 4.6 50.7 1.0 O1G B:ATP304 4.7 58.3 1.0 O B:PHE854 4.9 37.9 1.0 O1B B:ATP304 4.9 61.6 1.0 C B:ASP853 4.9 37.1 1.0

Magnesium binding site 4 out of 4 in the Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of EIF2ALPHA Protein Kinase GCN2: Wild-Type Complexed with Atp. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg308 b:42.2 occ:1.00 OD1 B:ASN840 2.1 40.9 1.0 O3B B:ATP304 2.4 56.5 1.0 OD2 B:ASP853 2.7 33.9 1.0 O3G B:ATP304 3.2 62.5 1.0 PG B:ATP304 3.2 52.4 1.0 CG B:ASN840 3.3 41.1 1.0 CG B:ASP853 3.5 36.6 1.0 PB B:ATP304 3.5 55.5 1.0 O2B B:ATP304 3.6 54.5 1.0 O1G B:ATP304 3.7 58.3 1.0 O3A B:ATP304 3.8 52.1 1.0 ND2 B:ASN840 3.8 42.3 1.0 CB B:ASP853 4.0 37.0 1.0 MG B:MG307 4.1 22.6 1.0 O B:MET839 4.1 45.4 1.0 O1A B:ATP304 4.2 47.4 1.0 OD1 B:ASP853 4.3 48.0 1.0 C2' B:ATP304 4.3 41.8 1.0 O2' B:ATP304 4.4 49.6 1.0 CB B:ASN840 4.5 32.8 1.0 O2G B:ATP304 4.6 59.8 1.0 PA B:ATP304 4.6 49.2 1.0 C B:MET839 4.6 43.4 1.0 CA B:ASN840 4.7 35.9 1.0 CE B:LYS837 4.8 39.2 1.0 C3' B:ATP304 4.9 43.4 1.0 N B:ASN840 4.9 40.2 1.0 O1B B:ATP304 4.9 61.6 1.0

A.K.Padyana, H.Qiu, A.Roll-Mecak, A.G.Hinnebusch, S.K.Burley. Structural Basis For Autoinhibition and Mutational Activation of Eukaryotic Initiation Factor 2{Alpha} Protein Kinase GCN2 J.Biol.Chem. V. 280 29289 2005.
ISSN: ISSN 0021-9258
PubMed: 15964839
DOI: 10.1074/JBC.M504096200