Magnesium in PDB 2a19: Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex.

The structure of Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex., PDB code: 2a19 was solved by A.C.Dar, T.E.Dever, F.Sicheri, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	27.33 / 2.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	64.659, 48.750, 133.434, 90.00, 98.44, 90.00
R / Rfree (%)	22.8 / 28.6

The binding sites of Magnesium atom in the Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex. (pdb code 2a19). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex., PDB code: 2a19:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1641 b:63.6 occ:1.00 OD2 B:ASP432 2.3 56.5 1.0 O2B B:ANP1640 2.5 91.2 1.0 OD1 B:ASP432 2.8 55.0 1.0 CG B:ASP432 2.9 55.6 1.0 PB B:ANP1640 3.7 90.3 1.0 O3A B:ANP1640 3.8 89.8 1.0 CA B:GLY434 4.1 53.7 1.0 NZ B:LYS296 4.2 69.0 1.0 MG B:MG1642 4.2 44.5 1.0 O1B B:ANP1640 4.4 90.6 1.0 CB B:ASP432 4.5 54.8 1.0 N B:GLY434 4.5 53.5 1.0 O2A B:ANP1640 4.5 85.7 1.0 PA B:ANP1640 4.7 87.7 1.0 CE B:LYS296 4.8 68.8 1.0 OD2 B:ASP414 4.9 59.9 1.0 O3G B:ANP1640 4.9 88.7 1.0 C B:GLY434 5.0 54.3 1.0 O1G B:ANP1640 5.0 89.9 1.0 O1A B:ANP1640 5.0 86.8 1.0

Magnesium binding site 2 out of 4 in the Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1642 b:44.5 occ:1.00 O1G B:ANP1640 2.0 89.9 1.0 O2A B:ANP1640 2.1 85.7 1.0 OD1 B:ASN419 2.3 50.7 1.0 OD2 B:ASP432 2.6 56.5 1.0 PG B:ANP1640 3.3 89.6 1.0 CG B:ASN419 3.3 52.5 1.0 O2B B:ANP1640 3.4 91.2 1.0 PA B:ANP1640 3.5 87.7 1.0 CG B:ASP432 3.6 55.6 1.0 N3B B:ANP1640 3.7 89.9 1.0 ND2 B:ASN419 3.7 51.1 1.0 CB B:ASP432 3.8 54.8 1.0 PB B:ANP1640 4.0 90.3 1.0 O3A B:ANP1640 4.0 89.8 1.0 O3G B:ANP1640 4.1 88.7 1.0 MG B:MG1641 4.2 63.6 1.0 O2G B:ANP1640 4.4 88.6 1.0 O1A B:ANP1640 4.5 86.8 1.0 O5' B:ANP1640 4.6 85.4 1.0 CB B:ASN419 4.7 53.2 1.0 OD1 B:ASP432 4.7 55.0 1.0 OD2 B:ASP414 4.8 59.9 1.0 C5' B:ANP1640 4.8 81.6 1.0 O B:SER418 4.8 54.5 1.0 OG B:SER418 4.9 54.4 1.0 CE B:LYS416 4.9 59.1 1.0 O3' B:ANP1640 5.0 76.8 1.0 CA B:ASN419 5.0 52.9 1.0

Magnesium binding site 3 out of 4 in the Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex. within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg2642 b:79.9 occ:1.00 O1A C:ANP2640 2.0 78.9 0.5 O3G C:ANP2640 2.0 78.5 0.5 OD1 C:ASN419 2.7 75.7 1.0 OD2 C:ASP432 2.7 76.5 1.0 PA C:ANP2640 3.0 79.5 0.5 MG C:MG2641 3.2 75.4 1.0 PG C:ANP2640 3.2 78.5 0.5 O2A C:ANP2640 3.3 78.9 0.5 N3B C:ANP2640 3.3 78.7 0.5 CG C:ASP432 3.6 76.8 1.0 O2B C:ANP2640 3.9 79.0 0.5 CG C:ASN419 3.9 75.2 1.0 O3A C:ANP2640 3.9 79.3 0.5 PB C:ANP2640 4.0 79.8 0.5 CB C:ASP432 4.1 76.4 1.0 O1G C:ANP2640 4.1 77.8 0.5 O2G C:ANP2640 4.3 78.4 0.5 O5' C:ANP2640 4.3 80.2 1.0 O C:SER418 4.5 75.7 1.0 OD1 C:ASP432 4.5 77.5 1.0 ND2 C:ASN419 4.6 74.9 1.0 OD2 C:ASP414 4.9 82.5 1.0 CB C:ASN419 5.0 75.0 1.0

Magnesium binding site 4 out of 4 in the Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Pkr Kinase Domain- EIF2ALPHA- Amp-Pnp Complex. within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg2641 b:75.4 occ:1.00 OD2 C:ASP432 2.2 76.5 1.0 N3B C:ANP2640 2.8 78.7 0.5 MG C:MG2642 3.2 79.9 1.0 OD2 C:ASP414 3.3 82.5 1.0 CG C:ASP432 3.3 76.8 1.0 O3G C:ANP2640 3.4 78.5 0.5 PG C:ANP2640 3.6 78.5 0.5 O2G C:ANP2640 3.7 78.4 0.5 OD1 C:ASP432 3.8 77.5 1.0 O2B C:ANP2640 3.9 79.0 0.5 PB C:ANP2640 4.0 79.8 0.5 O1A C:ANP2640 4.2 78.9 0.5 OD1 C:ASN419 4.3 75.7 1.0 CG C:ASP414 4.5 81.7 1.0 CB C:ASP432 4.5 76.4 1.0 O1G C:ANP2640 5.0 77.8 0.5

A.C.Dar, T.E.Dever, F.Sicheri. Higher-Order Substrate Recognition of EIF2ALPHA By the Rna-Dependent Protein Kinase Pkr. Cell(Cambridge,Mass.) V. 122 887 2005.
ISSN: ISSN 0092-8674
PubMed: 16179258
DOI: 10.1016/J.CELL.2005.06.044