Magnesium in PDB 2ai1: Purine Nucleoside Phosphorylase From Calf Spleen

All present enzymatic activity of Purine Nucleoside Phosphorylase From Calf Spleen:
2.4.2.1;

The structure of Purine Nucleoside Phosphorylase From Calf Spleen, PDB code: 2ai1 was solved by A.V.Toms, W.Wang, Y.Li, B.Ganem, S.E.Ealick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.36 / 2.00
Space group	P 21 3
Cell size a, b, c (Å), α, β, γ (°)	93.952, 93.952, 93.952, 90.00, 90.00, 90.00
R / Rfree (%)	21.2 / 25.4

The structure of Purine Nucleoside Phosphorylase From Calf Spleen also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Magnesium atom in the Purine Nucleoside Phosphorylase From Calf Spleen (pdb code 2ai1). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Purine Nucleoside Phosphorylase From Calf Spleen, PDB code: 2ai1:

Magnesium binding site 1 out of 1 in the Purine Nucleoside Phosphorylase From Calf Spleen


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Purine Nucleoside Phosphorylase From Calf Spleen within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg290 b:27.4 occ:1.00 O A:HOH2130 2.3 26.1 1.0 O A:HOH2128 2.3 24.8 1.0 O A:HOH2001 2.3 17.8 1.0 O A:HOH2002 2.3 18.0 1.0 O A:HOH2129 2.3 27.0 1.0 O A:HOH2000 2.4 17.8 1.0 O A:PHE56 3.9 23.6 1.0 O A:SER51 4.0 18.9 1.0 O A:HOH2003 4.1 20.2 1.0 O A:HOH2082 4.2 31.0 1.0 O A:HOH2064 4.2 33.7 1.0 O A:ILE53 4.2 17.9 1.0 O A:HOH2100 4.3 35.1 1.0 C A:SER51 4.7 19.6 1.0 CA A:SER51 4.8 19.3 1.0 O A:TYR50 4.9 13.3 1.0 N A:PHE56 4.9 21.8 1.0 N A:ASN55 4.9 21.2 1.0 C A:PHE56 5.0 23.9 1.0

A.V.Toms, W.Wang, Y.Li, B.Ganem, S.E.Ealick. Novel Multisubstrate Inhibitors of Mammalian Purine Nucleoside Phosphorylase. Acta Crystallogr.,Sect.D V. 61 1449 2005.
ISSN: ISSN 0907-4449
PubMed: 16239721
DOI: 10.1107/S0907444905025503