Magnesium in PDB 2aj4: Crystal Structure of Saccharomyces Cerevisiae Galactokinase in Complex with Galactose and Mg:Amppnp

All present enzymatic activity of Crystal Structure of Saccharomyces Cerevisiae Galactokinase in Complex with Galactose and Mg:Amppnp:
2.7.1.6;

The structure of Crystal Structure of Saccharomyces Cerevisiae Galactokinase in Complex with Galactose and Mg:Amppnp, PDB code: 2aj4 was solved by J.B.Thoden, C.A.Sellick, D.J.Timson, R.J.Reece, H.M.Holden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	63.300, 85.000, 112.300, 90.00, 96.70, 90.00
R / Rfree (%)	n/a / n/a

The structure of Crystal Structure of Saccharomyces Cerevisiae Galactokinase in Complex with Galactose and Mg:Amppnp also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Magnesium atom in the Crystal Structure of Saccharomyces Cerevisiae Galactokinase in Complex with Galactose and Mg:Amppnp (pdb code 2aj4). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Saccharomyces Cerevisiae Galactokinase in Complex with Galactose and Mg:Amppnp, PDB code: 2aj4:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Saccharomyces Cerevisiae Galactokinase in Complex with Galactose and Mg:Amppnp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Saccharomyces Cerevisiae Galactokinase in Complex with Galactose and Mg:Amppnp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg530 b:34.3 occ:1.00 O A:HOH565 2.7 29.0 1.0 O1B A:ANP532 2.9 14.1 1.0 O2G A:ANP532 2.9 17.8 1.0 O A:SER264 3.0 23.3 1.0 O A:HOH566 3.0 4.5 1.0 O A:HOH638 3.2 65.4 1.0 PG A:ANP532 3.8 18.2 1.0 CA A:GLY167 3.8 17.4 1.0 C A:SER264 3.9 26.9 1.0 O1G A:ANP532 3.9 16.1 1.0 N A:GLY167 4.1 21.1 1.0 N3B A:ANP532 4.1 31.0 1.0 CB A:ALA468 4.1 17.6 1.0 PB A:ANP532 4.1 21.7 1.0 N A:LYS266 4.2 12.8 1.0 OG A:SER264 4.2 26.1 1.0 CB A:LYS266 4.3 20.2 1.0 CB A:SER264 4.4 21.7 1.0 CG A:LYS266 4.5 17.9 1.0 N A:ASN265 4.7 14.1 1.0 CA A:SER264 4.7 25.5 1.0 CA A:LYS266 4.7 14.8 1.0 O A:HOH567 4.8 21.5 1.0 CA A:ASN265 4.8 12.2 1.0 N A:ALA468 4.8 19.4 1.0 CD A:LYS266 4.8 23.5 1.0 C A:ASN265 4.8 19.9 1.0 O A:GLY469 5.0 14.5 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Saccharomyces Cerevisiae Galactokinase in Complex with Galactose and Mg:Amppnp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Saccharomyces Cerevisiae Galactokinase in Complex with Galactose and Mg:Amppnp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg530 b:44.4 occ:1.00 O2G B:ANP532 2.8 15.3 1.0 O B:SER264 2.8 25.1 1.0 O B:HOH550 2.9 18.9 1.0 O1B B:ANP532 2.9 15.5 1.0 O B:HOH551 3.6 16.5 1.0 C B:SER264 3.7 31.3 1.0 PG B:ANP532 3.8 28.5 1.0 O1G B:ANP532 3.9 9.5 1.0 CB B:SER264 3.9 32.9 1.0 CA B:GLY167 4.0 20.6 1.0 OG B:SER264 4.1 34.8 1.0 N B:LYS266 4.1 19.6 1.0 PB B:ANP532 4.1 26.9 1.0 N B:GLY167 4.1 27.8 1.0 N3B B:ANP532 4.2 25.5 1.0 CB B:ALA468 4.4 15.6 1.0 CB B:LYS266 4.4 26.3 1.0 CA B:SER264 4.4 30.6 1.0 N B:ASN265 4.5 21.9 1.0 CA B:ASN265 4.6 15.9 1.0 C B:ASN265 4.7 24.3 1.0 CA B:LYS266 4.8 23.7 1.0 CA B:GLY165 4.8 29.6 1.0 C B:GLY165 4.8 26.2 1.0 O B:GLY165 4.9 17.3 1.0 CG B:LYS266 5.0 15.1 1.0 O B:HOH552 5.0 20.1 1.0 O B:GLY469 5.0 16.0 1.0

J.B.Thoden, C.A.Sellick, D.J.Timson, R.J.Reece, H.M.Holden. Molecular Structure of Saccharomyces Cerevisiae GAL1P, A Bifunctional Galactokinase and Transcriptional Inducer J.Biol.Chem. V. 280 36905 2005.
ISSN: ISSN 0021-9258
PubMed: 16115868
DOI: 10.1074/JBC.M508446200