Magnesium in PDB 2awj: Gfp R96M Pre-Cyclized Intermediate in Chromophore Formation

The structure of Gfp R96M Pre-Cyclized Intermediate in Chromophore Formation, PDB code: 2awj was solved by T.I.Wood, D.P.Barondeau, C.Hitomi, C.J.Kassmann, J.A.Tainer, E.D.Getzoff, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.196, 62.771, 71.430, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 24.1

The binding sites of Magnesium atom in the Gfp R96M Pre-Cyclized Intermediate in Chromophore Formation (pdb code 2awj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Gfp R96M Pre-Cyclized Intermediate in Chromophore Formation, PDB code: 2awj:

Magnesium binding site 1 out of 1 in the Gfp R96M Pre-Cyclized Intermediate in Chromophore Formation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Gfp R96M Pre-Cyclized Intermediate in Chromophore Formation within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg300 b:23.6 occ:1.00 O A:HOH1367 1.9 16.2 1.0 O A:HOH1365 2.0 13.2 1.0 O A:HOH1369 2.3 16.2 1.0 O A:HOH1370 2.9 26.3 1.0 O A:HOH1368 2.9 21.0 1.0 O A:HOH1366 3.0 44.7 1.0 O A:HOH1308 4.0 50.0 1.0 OD2 A:ASP197 4.0 18.6 1.0 OD1 A:ASP197 4.0 16.6 1.0 O A:HOH1249 4.3 33.4 1.0 CG A:ASP197 4.4 22.8 1.0 O A:HOH1081 4.7 41.2 1.0

T.I.Wood, D.P.Barondeau, C.Hitomi, C.J.Kassmann, J.A.Tainer, E.D.Getzoff. Defining the Role of Arginine 96 in Green Fluorescent Protein Fluorophore Biosynthesis. Biochemistry V. 44 16211 2005.
ISSN: ISSN 0006-2960
PubMed: 16331981
DOI: 10.1021/BI051388J