Magnesium in PDB 2awm: Gfp R96A Chromophore Maturation Recovery Mutant R96A Q183R

The structure of Gfp R96A Chromophore Maturation Recovery Mutant R96A Q183R, PDB code: 2awm was solved by T.I.Wood, D.P.Barondeau, C.Hitomi, C.J.Kassmann, J.A.Tainer, E.D.Getzoff, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.510, 62.340, 71.260, 90.00, 90.00, 90.00
R / Rfree (%)	22.3 / 22.3

The binding sites of Magnesium atom in the Gfp R96A Chromophore Maturation Recovery Mutant R96A Q183R (pdb code 2awm). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Gfp R96A Chromophore Maturation Recovery Mutant R96A Q183R, PDB code: 2awm:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Gfp R96A Chromophore Maturation Recovery Mutant R96A Q183R


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Gfp R96A Chromophore Maturation Recovery Mutant R96A Q183R within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg300 b:23.7 occ:1.00 O A:HOH1301 2.1 17.1 1.0 O A:HOH1299 2.2 18.0 1.0 O A:HOH1302 2.2 12.9 1.0 O A:HOH1303 2.3 13.0 1.0 O A:HOH1300 2.3 12.6 1.0 O A:HOH1304 2.3 23.0 1.0 O A:HOH1042 3.8 19.1 1.0 OE1 A:GLU142 3.9 15.8 1.0 OE2 A:GLU142 4.1 12.7 1.0 CA A:GLY174 4.2 9.1 1.0 CD A:GLU142 4.4 14.3 1.0 O A:HOH1175 4.5 30.4 1.0 N A:GLY174 4.8 16.7 1.0 O A:ILE171 5.0 16.0 1.0

Magnesium binding site 2 out of 2 in the Gfp R96A Chromophore Maturation Recovery Mutant R96A Q183R


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Gfp R96A Chromophore Maturation Recovery Mutant R96A Q183R within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:42.6 occ:1.00 O A:HOH1306 2.1 13.6 1.0 O A:HOH1305 2.3 26.6 1.0 O A:HOH1307 2.3 27.0 1.0 O A:HOH1310 2.5 31.8 1.0 O A:HOH1308 2.7 69.9 1.0 OG A:SER30 3.8 22.9 1.0 OE1 A:GLU17 4.1 12.6 1.0 O A:HOH1030 4.2 16.4 1.0 O A:HOH1248 4.3 19.9 1.0 CB A:SER30 4.4 14.8 1.0 OE2 A:GLU17 4.7 15.5 1.0 CD A:GLU17 4.8 12.1 1.0

T.I.Wood, D.P.Barondeau, C.Hitomi, C.J.Kassmann, J.A.Tainer, E.D.Getzoff. Defining the Role of Arginine 96 in Green Fluorescent Protein Fluorophore Biosynthesis. Biochemistry V. 44 16211 2005.
ISSN: ISSN 0006-2960
PubMed: 16331981
DOI: 10.1021/BI051388J