Magnesium in PDB 2bhz: Crystal Structure of Deinococcus Radiodurans Maltooligosyltrehalose Trehalohydrolase in Complex with Maltose

All present enzymatic activity of Crystal Structure of Deinococcus Radiodurans Maltooligosyltrehalose Trehalohydrolase in Complex with Maltose:
3.2.1.1;

The structure of Crystal Structure of Deinococcus Radiodurans Maltooligosyltrehalose Trehalohydrolase in Complex with Maltose, PDB code: 2bhz was solved by J.Timmins, H.-K.S.Leiros, G.Leonard, I.Leiros, S.Mcsweeney, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	76.70 / 1.2
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	59.145, 66.565, 153.501, 90.00, 90.00, 90.00
R / Rfree (%)	12.5 / 14.2

The binding sites of Magnesium atom in the Crystal Structure of Deinococcus Radiodurans Maltooligosyltrehalose Trehalohydrolase in Complex with Maltose (pdb code 2bhz). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Deinococcus Radiodurans Maltooligosyltrehalose Trehalohydrolase in Complex with Maltose, PDB code: 2bhz:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Deinococcus Radiodurans Maltooligosyltrehalose Trehalohydrolase in Complex with Maltose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Deinococcus Radiodurans Maltooligosyltrehalose Trehalohydrolase in Complex with Maltose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1612 b:10.1 occ:1.00 OD1 A:ASP540 2.1 9.6 1.0 O A:HOH2835 2.1 13.1 1.0 CG A:ASP540 3.1 8.8 1.0 OD2 A:ASP540 3.5 10.8 1.0 N A:GLY541 4.2 7.8 1.0 O A:HOH2838 4.2 27.8 1.0 NZ A:LYS572 4.2 16.5 0.5 O A:HOH2840 4.3 11.9 1.0 O A:HOH2837 4.3 11.5 1.0 C A:ASP540 4.4 7.7 1.0 CB A:ASP540 4.4 8.2 1.0 CE A:LYS572 4.5 17.1 0.5 CD A:LYS572 4.6 16.7 0.5 O A:HOH2881 4.6 29.2 1.0 CA A:ASP540 4.7 7.9 1.0 CA A:GLY541 4.8 7.6 1.0 O A:HOH2836 4.9 25.6 1.0 O A:ASP540 4.9 7.5 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Deinococcus Radiodurans Maltooligosyltrehalose Trehalohydrolase in Complex with Maltose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Deinococcus Radiodurans Maltooligosyltrehalose Trehalohydrolase in Complex with Maltose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1613 b:13.2 occ:0.70 O A:HOH2560 2.0 19.6 1.0 O A:HOH2944 2.0 14.1 1.0 O A:HOH2559 2.0 12.8 1.0 O A:HOH2667 2.1 11.8 1.0 OD2 A:ASP400 2.2 11.0 1.0 OE1 A:GLU308 2.5 15.0 1.0 CG A:ASP400 3.2 7.8 1.0 CD A:GLU308 3.3 10.7 1.0 OE2 A:GLU308 3.4 10.9 1.0 OD1 A:ASP400 3.5 9.0 1.0 O A:HOH2255 3.6 35.0 1.0 O A:HOH2558 3.8 12.3 1.0 O A:HOH2947 3.8 27.0 1.0 O A:HOH2937 3.8 23.9 1.0 OD1 A:ASP275 3.9 9.1 1.0 O A:HOH2942 3.9 35.6 1.0 O A:HOH2948 4.0 42.4 1.0 NH2 A:ARG273 4.2 8.0 1.0 CE1 A:HIS399 4.3 7.8 1.0 O1' A:MAL1604 4.5 16.8 0.7 CB A:ASP400 4.5 6.6 1.0 O A:HOH2936 4.7 19.9 1.0 CG A:GLU308 4.7 8.2 1.0 NE2 A:HIS399 5.0 10.2 1.0 CG A:ASP275 5.0 7.9 1.0 O2' A:MAL1603 5.0 12.7 0.7

J.Timmins, H.-K.S.Leiros, G.Leonard, I.Leiros, S.Mcsweeney. Crystal Structure of Maltooligosyltrehalose Trehalohydrolase From Deinococcus Radiodurans in Complex with Disaccharides J.Mol.Biol. V. 347 949 2005.
ISSN: ISSN 0022-2836
PubMed: 15784255
DOI: 10.1016/J.JMB.2005.02.011