Magnesium in PDB 2c4r: Catalytic Domain of E. Coli Rnase E

The structure of Catalytic Domain of E. Coli Rnase E, PDB code: 2c4r was solved by M.J.Marcaida, A.J.Callaghan, B.F.Luisi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 3.60
Space group	P 62 2 2
Cell size a, b, c (Å), α, β, γ (°)	196.586, 196.586, 140.766, 90.00, 90.00, 120.00
R / Rfree (%)	31.9 / 34.7

The structure of Catalytic Domain of E. Coli Rnase E also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Magnesium atom in the Catalytic Domain of E. Coli Rnase E (pdb code 2c4r). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Catalytic Domain of E. Coli Rnase E, PDB code: 2c4r:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Catalytic Domain of E. Coli Rnase E


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Catalytic Domain of E. Coli Rnase E within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg1510 b:48.1 occ:0.50 O L:THR410 3.8 60.0 1.0 C L:THR410 4.5 66.3 1.0 O L:SER295 4.6 59.0 1.0 O L:GLY409 4.7 84.0 1.0 OD1 L:ASP294 4.8 70.8 1.0 CA L:THR410 4.9 68.3 1.0

Magnesium binding site 2 out of 2 in the Catalytic Domain of E. Coli Rnase E


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Catalytic Domain of E. Coli Rnase E within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg1511 b:52.1 occ:1.00 O L:HOH2004 2.2 50.3 1.0 O L:HOH2001 2.2 52.6 1.0 O L:HOH2003 2.2 44.5 1.0 O L:HOH2002 2.2 49.6 1.0 O L:HOH2005 2.2 50.5 1.0 OD2 L:ASP346 2.6 66.7 1.0 OD2 L:ASP303 2.8 60.4 1.0 O L:ILE304 3.7 42.8 1.0 CG L:ASP346 3.8 62.5 1.0 CG L:ASP303 3.9 60.2 1.0 OD1 L:ASP303 4.3 65.0 1.0 OD1 L:ASP346 4.4 64.9 1.0 O L:ASP346 4.6 51.5 1.0 C L:ILE304 4.9 42.0 1.0 CB L:ASP346 4.9 56.6 1.0 CD1 L:ILE348 5.0 43.8 1.0

A.J.Callaghan, M.J.Marcaida, J.A.Stead, K.J.Mcdowall, W.G.Scott, B.F.Luisi. Structure of E. Coli Rnase E Catalytic Domain and Implications For Rna Processing and Turnover Nature V. 437 1187 2005.
ISSN: ISSN 0028-0836
PubMed: 16237448
DOI: 10.1038/NATURE04084