Magnesium in PDB 2c78: Ef-Tu Complexed with A Gtp Analog and the Antibiotic Pulvomycin

All present enzymatic activity of Ef-Tu Complexed with A Gtp Analog and the Antibiotic Pulvomycin:
3.1.5.1;

The structure of Ef-Tu Complexed with A Gtp Analog and the Antibiotic Pulvomycin, PDB code: 2c78 was solved by A.Parmeggiani, I.M.Krab, S.Okamura, R.C.Nielsen, J.Nyborg, P.Nissen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	N/A / 1.40
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	86.686, 86.686, 105.514, 90.00, 90.00, 120.00
R / Rfree (%)	19.7 / 21.6

The binding sites of Magnesium atom in the Ef-Tu Complexed with A Gtp Analog and the Antibiotic Pulvomycin (pdb code 2c78). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Ef-Tu Complexed with A Gtp Analog and the Antibiotic Pulvomycin, PDB code: 2c78:

Magnesium binding site 1 out of 1 in the Ef-Tu Complexed with A Gtp Analog and the Antibiotic Pulvomycin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Ef-Tu Complexed with A Gtp Analog and the Antibiotic Pulvomycin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1407 b:10.5 occ:1.00 O1G A:GNP1406 2.0 11.6 1.0 OG1 A:THR62 2.0 11.7 1.0 O A:HOH2035 2.1 11.5 1.0 O A:HOH2010 2.1 9.5 1.0 OG1 A:THR25 2.1 9.8 1.0 O2B A:GNP1406 2.1 9.8 1.0 CB A:THR62 3.1 9.6 1.0 PG A:GNP1406 3.2 10.2 1.0 CB A:THR25 3.2 9.8 1.0 PB A:GNP1406 3.2 10.2 1.0 N3B A:GNP1406 3.3 11.3 1.0 N A:THR25 3.8 8.8 1.0 O3G A:GNP1406 4.0 11.6 1.0 CG2 A:THR62 4.1 11.7 1.0 OD1 A:ASP81 4.1 11.0 1.0 CA A:THR25 4.1 10.2 1.0 OD2 A:ASP51 4.1 13.2 1.0 N A:THR62 4.1 11.3 1.0 OD1 A:ASP51 4.1 13.0 1.0 CA A:THR62 4.1 10.7 1.0 O1B A:GNP1406 4.2 10.6 1.0 O2A A:GNP1406 4.2 11.2 1.0 O2G A:GNP1406 4.3 10.7 1.0 CG2 A:THR25 4.3 11.2 1.0 O3A A:GNP1406 4.4 11.0 1.0 OD2 A:ASP81 4.4 10.2 1.0 CB A:LYS24 4.5 9.2 1.0 O A:CYS82 4.5 10.4 1.0 CG A:ASP51 4.5 11.7 1.0 CE A:LYS24 4.6 9.5 1.0 CG A:ASP81 4.6 9.9 1.0 PA A:GNP1406 4.7 10.7 1.0 C A:LYS24 4.8 8.7 1.0 O1A A:GNP1406 4.8 10.7 1.0

A.Parmeggiani, I.M.Krab, S.Okamura, R.C.Nielsen, J.Nyborg, P.Nissen. Structural Basis of the Action of Pulvomycin and GE2270 A on Elongation Factor Tu. Biochemistry V. 45 6846 2006.
ISSN: ISSN 0006-2960
PubMed: 16734421
DOI: 10.1021/BI0525122