Magnesium in PDB 2chg: Replication Factor C Domains 1 and 2

The structure of Replication Factor C Domains 1 and 2, PDB code: 2chg was solved by A.Seybert, M.R.Singleton, N.Cook, D.R.Hall, D.B.Wigley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.96 / 2.1
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	77.504, 91.472, 140.644, 90.00, 90.00, 90.00
R / Rfree (%)	21.2 / 25.6

The binding sites of Magnesium atom in the Replication Factor C Domains 1 and 2 (pdb code 2chg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Replication Factor C Domains 1 and 2, PDB code: 2chg:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Replication Factor C Domains 1 and 2


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Replication Factor C Domains 1 and 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1228 b:34.0 occ:1.00 O A:HOH2062 1.9 27.4 1.0 O A:HOH2059 1.9 19.3 1.0 O1G A:ANP1227 1.9 25.8 1.0 O A:HOH2063 2.0 28.8 1.0 O A:HOH2061 2.1 29.5 1.0 O2B A:ANP1227 2.1 26.3 1.0 PG A:ANP1227 3.1 27.3 1.0 PB A:ANP1227 3.3 28.6 1.0 O2G A:ANP1227 3.5 26.3 1.0 N3B A:ANP1227 3.5 27.9 1.0 N A:GLY48 3.9 27.2 1.0 O A:HOH2011 4.0 27.4 1.0 O A:HOH2041 4.1 23.3 1.0 O1B A:ANP1227 4.1 28.2 1.0 O3G A:ANP1227 4.4 25.9 1.0 CA A:GLY48 4.5 26.8 1.0 O3A A:ANP1227 4.5 24.3 1.0 O A:HOH2021 4.6 35.3 1.0 CA A:PRO47 4.7 27.6 1.0 C A:PRO47 4.7 27.3 1.0 CB A:PRO47 4.8 27.8 1.0 NH1 A:ARG198 4.9 34.1 1.0

Magnesium binding site 2 out of 4 in the Replication Factor C Domains 1 and 2


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Replication Factor C Domains 1 and 2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1227 b:34.3 occ:1.00 O B:HOH2059 1.9 27.5 1.0 O B:HOH2057 2.0 28.6 1.0 O1G B:ANP1226 2.1 26.5 1.0 O B:HOH2055 2.1 31.5 1.0 O B:HOH2061 2.1 26.4 1.0 O2B B:ANP1226 2.1 25.7 1.0 PG B:ANP1226 3.2 26.6 1.0 PB B:ANP1226 3.4 26.5 1.0 O2G B:ANP1226 3.5 27.5 1.0 N3B B:ANP1226 3.6 27.0 1.0 N B:GLY48 4.0 31.9 1.0 O B:HOH2056 4.2 24.6 1.0 O1B B:ANP1226 4.3 27.7 1.0 O3G B:ANP1226 4.5 31.3 1.0 O B:HOH2021 4.5 30.9 1.0 O3A B:ANP1226 4.6 25.4 1.0 CA B:GLY48 4.6 30.3 1.0 OD2 B:ASP109 4.8 36.9 1.0 CA B:PRO47 4.8 33.0 1.0 CB B:PRO47 4.8 33.8 1.0 C B:PRO47 4.9 32.5 1.0

Magnesium binding site 3 out of 4 in the Replication Factor C Domains 1 and 2


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Replication Factor C Domains 1 and 2 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg1226 b:47.3 occ:1.00 O C:HOH2038 1.9 29.9 1.0 O C:HOH2037 1.9 29.3 1.0 O C:HOH2041 2.0 32.1 1.0 O2B C:ANP1225 2.2 30.6 1.0 O1G C:ANP1225 2.2 32.0 1.0 PG C:ANP1225 3.3 31.4 1.0 PB C:ANP1225 3.5 31.8 1.0 O2G C:ANP1225 3.6 34.4 1.0 N3B C:ANP1225 3.7 30.4 1.0 O C:HOH2031 4.2 29.1 1.0 O1B C:ANP1225 4.4 31.1 1.0 N C:GLY48 4.4 37.3 1.0 O3A C:ANP1225 4.6 30.1 1.0 OD2 C:ASP109 4.7 41.6 1.0 O3G C:ANP1225 4.7 35.1 1.0 O C:HOH2008 4.8 34.0 1.0 CA C:GLY48 4.8 36.1 1.0

Magnesium binding site 4 out of 4 in the Replication Factor C Domains 1 and 2


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Replication Factor C Domains 1 and 2 within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1226 b:42.4 occ:1.00 O D:HOH2050 1.9 30.4 1.0 O D:HOH2049 2.0 29.3 1.0 O D:HOH2048 2.1 28.4 1.0 O1G D:ANP1225 2.2 27.4 1.0 O2B D:ANP1225 2.4 31.8 1.0 PG D:ANP1225 3.3 30.0 1.0 PB D:ANP1225 3.5 33.6 1.0 O D:HOH2009 3.6 42.2 1.0 N3B D:ANP1225 3.8 30.9 1.0 O2G D:ANP1225 3.8 28.3 1.0 O D:HOH2046 4.1 21.9 1.0 N D:GLY48 4.1 35.5 1.0 O1B D:ANP1225 4.3 32.4 1.0 CB D:PRO47 4.4 37.7 1.0 O3G D:ANP1225 4.6 32.4 1.0 CA D:GLY48 4.7 33.6 1.0 CA D:PRO47 4.8 37.3 1.0 O3A D:ANP1225 4.8 29.4 1.0 C D:PRO47 4.9 36.6 1.0 OD2 D:ASP109 4.9 34.3 1.0

A.Seybert, M.R.Singleton, N.Cook, D.R.Hall, D.B.Wigley. Communication Between Subunits Within An Archaeal Clamp-Loader Complex. Embo J. V. 25 2209 2006.
ISSN: ISSN 0261-4189
PubMed: 16628222
DOI: 10.1038/SJ.EMBOJ.7601093