Magnesium in PDB 2cl7: Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp

The structure of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp, PDB code: 2cl7 was solved by B.U.Klink, R.S.Goody, A.J.Scheidig, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	69.34 / 1.25
Space group	P 41
Cell size a, b, c (Å), α, β, γ (°)	69.280, 69.280, 35.020, 90.00, 90.00, 90.00
R / Rfree (%)	14.8 / 17

The binding sites of Magnesium atom in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp (pdb code 2cl7). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp, PDB code: 2cl7:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg168 b:4.6 occ:0.50 OG1 X:THR35 1.9 30.2 0.5 O X:HOH2176 2.1 9.5 0.5 O1B X:GTP1167 2.1 5.5 0.5 O X:HOH2170 2.1 9.6 0.5 OG X:SER17 2.2 5.5 0.5 O1G X:GTP1167 2.4 8.5 0.5 CB X:THR35 2.9 32.2 0.5 CB X:SER17 3.2 5.1 0.5 PB X:GTP1167 3.4 6.3 0.5 CG2 X:THR35 3.5 32.4 0.5 PG X:GTP1167 3.6 9.0 0.5 OD2 X:ASP57 3.7 7.7 0.5 O3B X:GTP1167 3.8 5.8 0.5 OD1 X:ASP57 4.0 5.4 0.5 N X:SER17 4.0 4.8 0.5 CA X:THR35 4.2 32.2 0.5 O1A X:GTP1167 4.2 6.3 0.5 CA X:SER17 4.2 4.2 0.5 O X:HOH2064 4.3 31.3 0.5 CG X:ASP57 4.3 7.0 0.5 O3A X:GTP1167 4.4 7.4 0.5 O2G X:GTP1167 4.5 10.3 0.5 N X:THR35 4.5 32.6 0.5 O2A X:GTP1167 4.5 8.2 0.5 O2B X:GTP1167 4.5 6.5 0.5 PA X:GTP1167 4.5 6.8 0.5 O X:THR58 4.5 9.8 0.5 OD2 X:ASP33 4.6 34.1 0.5 O3G X:GTP1167 4.7 8.6 0.5 O X:ASP33 4.7 33.7 0.5 O X:HOH2102 4.8 42.4 0.5 CB X:LYS16 4.9 4.8 0.5

Magnesium binding site 2 out of 2 in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg169 b:13.7 occ:0.50 OD1 X:ASP108 1.9 12.5 0.5 O X:HOH2026 1.9 16.8 0.5 O X:HOH2119 2.2 30.9 0.5 NE2 X:HIS166 2.3 11.0 0.5 O X:HOH2171 2.3 29.2 0.5 CG X:ASP108 2.8 11.9 0.5 O X:HOH2143 2.9 33.2 0.5 OD2 X:ASP108 3.1 16.9 0.5 CE1 X:HIS166 3.2 9.5 0.5 CD2 X:HIS166 3.3 8.8 0.5 O X:HOH2177 3.9 29.7 0.5 O X:HOH2002 4.1 8.6 0.5 CB X:ASP108 4.3 11.6 0.5 ND1 X:HIS166 4.4 5.9 0.5 CG X:HIS166 4.5 8.7 0.5 O X:GLY138 4.7 5.1 0.5 CA X:ASP108 4.7 10.0 0.5 OE1 X:GLU162 4.7 6.7 0.5 O X:HOH2032 4.8 23.5 0.5 O X:HOH2015 4.9 25.1 0.5 O X:VAL109 4.9 4.3 0.5 C X:ASP108 5.0 9.9 0.5

B.U.Klink, R.S.Goody, A.J.Scheidig. A Newly Designed Microspectrofluorometer For Kinetic Studies on Protein Crystals in Combination with X-Ray Diffraction. Biophys.J. V. 91 981 2006.
ISSN: ISSN 0006-3495
PubMed: 16698776
DOI: 10.1529/BIOPHYSJ.105.078931