Magnesium in PDB 2clc: Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp (2)

The structure of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp (2), PDB code: 2clc was solved by B.U.Klink, R.S.Goody, A.J.Scheidig, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	69.34 / 1.30
Space group	P 41
Cell size a, b, c (Å), α, β, γ (°)	69.320, 69.320, 35.010, 90.00, 90.00, 90.00
R / Rfree (%)	14.9 / 18

The binding sites of Magnesium atom in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp (2) (pdb code 2clc). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp (2), PDB code: 2clc:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp (2)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp (2) within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg168 b:10.2 occ:0.50 OG1 X:THR35 2.0 30.1 0.5 O X:HOH2170 2.1 2.1 0.5 OG X:SER17 2.1 12.8 0.5 O1B X:GTP1167 2.1 12.4 0.5 O X:HOH2176 2.2 2.7 0.5 O1G X:GTP1167 2.3 11.5 0.5 CB X:THR35 3.1 33.5 0.5 CB X:SER17 3.1 11.1 0.5 PB X:GTP1167 3.4 12.5 0.5 PG X:GTP1167 3.6 14.2 0.5 O3B X:GTP1167 3.7 11.0 0.5 OD2 X:ASP57 3.8 14.8 0.5 OD1 X:ASP57 3.9 13.6 0.5 N X:SER17 4.0 10.1 0.5 CG2 X:THR35 4.0 33.9 0.5 CA X:SER17 4.1 11.1 0.5 O1A X:GTP1167 4.1 12.1 0.5 CA X:THR35 4.3 33.6 0.5 N X:THR35 4.3 34.2 0.5 O X:HOH2064 4.3 27.0 0.5 CG X:ASP57 4.3 12.8 0.5 O3A X:GTP1167 4.4 13.2 0.5 O X:ASP33 4.4 36.4 0.5 O2A X:GTP1167 4.4 14.1 0.5 PA X:GTP1167 4.4 12.0 0.5 O X:THR58 4.4 15.4 0.5 O2B X:GTP1167 4.5 11.0 0.5 O2G X:GTP1167 4.5 14.5 0.5 O3G X:GTP1167 4.5 15.5 0.5 OD2 X:ASP33 4.6 36.7 0.5 CB X:LYS16 4.9 9.8 0.5 CE X:LYS16 5.0 11.7 0.5 C X:LYS16 5.0 10.3 0.5

Magnesium binding site 2 out of 2 in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp (2)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gtp (2) within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg169 b:21.5 occ:0.50 OD1 X:ASP108 1.7 13.1 0.5 O X:HOH2026 1.9 4.7 0.5 NE2 X:HIS166 2.2 13.5 0.5 O X:HOH2119 2.3 14.3 0.5 O X:HOH2171 2.4 13.3 0.5 O X:HOH2174 2.8 17.3 0.5 CG X:ASP108 2.8 16.5 0.5 CD2 X:HIS166 3.1 10.0 0.5 OD2 X:ASP108 3.2 19.9 0.5 CE1 X:HIS166 3.3 9.6 0.5 O X:HOH2177 3.7 36.4 0.5 O X:HOH2002 4.1 2.0 0.5 CB X:ASP108 4.2 16.8 0.5 CG X:HIS166 4.3 12.1 0.5 ND1 X:HIS166 4.4 10.0 0.5 CA X:ASP108 4.5 15.5 0.5 O X:VAL109 4.7 11.5 0.5 O X:GLY138 4.7 11.2 0.5 OE1 X:GLU162 4.7 13.7 0.5 C X:ASP108 4.8 15.4 0.5

B.U.Klink, R.S.Goody, A.J.Scheidig. A Newly Designed Microspectrofluorometer For Kinetic Studies on Protein Crystals in Combination with X-Ray Diffraction Biophys.J. V. 91 981 2006.
ISSN: ISSN 0006-3495
PubMed: 16698776
DOI: 10.1529/BIOPHYSJ.105.078931