Magnesium in PDB 2e8a: Crystal Structure of the Human HSP70 Atpase Domain in Complex with Amp-Pnp

The structure of Crystal Structure of the Human HSP70 Atpase Domain in Complex with Amp-Pnp, PDB code: 2e8a was solved by M.Shida, R.Ishii, T.Takagi, S.Kishishita, M.Shirouzu, S.Yokoyama, Riken Structural Genomics/Proteomics Initiative (Rsgi), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.94 / 1.77
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	45.490, 62.220, 143.040, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 23

The binding sites of Magnesium atom in the Crystal Structure of the Human HSP70 Atpase Domain in Complex with Amp-Pnp (pdb code 2e8a). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of the Human HSP70 Atpase Domain in Complex with Amp-Pnp, PDB code: 2e8a:

Magnesium binding site 1 out of 1 in the Crystal Structure of the Human HSP70 Atpase Domain in Complex with Amp-Pnp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Human HSP70 Atpase Domain in Complex with Amp-Pnp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:28.6 occ:1.00 O1B A:ANP601 2.1 13.5 1.0 O A:HOH626 2.2 16.5 1.0 OD2 A:ASP10 2.3 18.6 1.0 O A:TYR15 2.4 11.7 1.0 CG A:ASP10 3.1 17.4 1.0 PB A:ANP601 3.2 15.5 1.0 O A:HOH775 3.3 26.8 1.0 C A:TYR15 3.4 13.4 1.0 OD1 A:ASP10 3.5 15.8 1.0 O A:HOH630 3.6 16.6 1.0 O3A A:ANP601 3.7 15.4 1.0 O2B A:ANP601 3.8 13.1 1.0 O2A A:ANP601 3.9 13.6 1.0 CA A:GLY12 3.9 14.5 1.0 O1A A:ANP601 4.0 13.7 1.0 PA A:ANP601 4.0 13.1 1.0 CA A:SER16 4.1 7.2 1.0 N A:SER16 4.2 8.4 1.0 N A:GLY12 4.2 13.2 1.0 CB A:ASP10 4.3 13.2 1.0 CA A:TYR15 4.4 11.3 1.0 CB A:TYR15 4.4 12.8 1.0 O A:ASP10 4.5 12.8 1.0 O A:HOH605 4.5 9.9 1.0 O A:ASP366 4.5 14.0 1.0 N3B A:ANP601 4.6 17.3 1.0 C A:SER16 4.6 10.3 1.0 N A:TYR15 4.6 11.0 1.0 N A:CYS17 4.8 11.2 1.0 C A:ASP10 4.9 11.4 1.0

M.Shida, A.Arakawa, R.Ishii, S.Kishishita, T.Takagi, M.Kukimoto-Niino, S.Sugano, A.Tanaka, M.Shirouzu, S.Yokoyama. Direct Inter-Subdomain Interactions Switch Between the Closed and Open Forms of the HSP70 Nucleotide-Binding Domain in the Nucleotide-Free State. Acta Crystallogr.,Sect.D V. 66 223 2010.
ISSN: ISSN 0907-4449
PubMed: 20179333
DOI: 10.1107/S0907444909053979