Magnesium in PDB 2enx: Structure of the Family II Inorganic Pyrophosphatase From Streptococcus Agalactiae at 2.8 Resolution

All present enzymatic activity of Structure of the Family II Inorganic Pyrophosphatase From Streptococcus Agalactiae at 2.8 Resolution:
3.6.1.1;

The structure of Structure of the Family II Inorganic Pyrophosphatase From Streptococcus Agalactiae at 2.8 Resolution, PDB code: 2enx was solved by M.K.Rantanen, L.Lehtio, L.Rajagopal, C.E.Rubens, A.Goldman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.50 / 2.80
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	181.960, 181.960, 132.610, 90.00, 90.00, 120.00
R / Rfree (%)	19.2 / 26.7

The structure of Structure of the Family II Inorganic Pyrophosphatase From Streptococcus Agalactiae at 2.8 Resolution also contains other interesting chemical elements:

Manganese

(Mn)

4 atoms

The binding sites of Magnesium atom in the Structure of the Family II Inorganic Pyrophosphatase From Streptococcus Agalactiae at 2.8 Resolution (pdb code 2enx). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of the Family II Inorganic Pyrophosphatase From Streptococcus Agalactiae at 2.8 Resolution, PDB code: 2enx:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structure of the Family II Inorganic Pyrophosphatase From Streptococcus Agalactiae at 2.8 Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of the Family II Inorganic Pyrophosphatase From Streptococcus Agalactiae at 2.8 Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:17.7 occ:1.00 O3 A:2PN1001 2.1 17.7 1.0 O6 A:2PN1001 2.5 17.8 1.0 O A:HOH1099 2.6 17.8 1.0 P1 A:2PN1001 3.4 18.5 1.0 P2 A:2PN1001 3.4 18.2 1.0 NH2 A:ARG297 3.5 18.4 1.0 O5 A:2PN1001 3.5 18.1 1.0 N1 A:2PN1001 3.8 18.5 1.0 OD2 A:ASP205 3.9 18.3 1.0 O2 A:2PN1001 3.9 18.4 1.0 OE1 A:GLN220 4.3 18.2 1.0 OD2 A:ASP13 4.4 17.7 1.0 OD1 A:ASP205 4.5 17.9 1.0 CZ A:ARG297 4.5 18.2 1.0 OD1 A:ASP151 4.5 17.9 1.0 O1 A:2PN1001 4.6 18.0 1.0 O A:HOH1085 4.6 18.3 1.0 CG A:ASP205 4.6 18.1 1.0 O4 A:2PN1001 4.8 18.1 1.0 CG A:ASP13 5.0 18.6 1.0 NE A:ARG297 5.0 17.8 1.0 MN A:MN401 5.0 17.9 1.0

Magnesium binding site 2 out of 2 in the Structure of the Family II Inorganic Pyrophosphatase From Streptococcus Agalactiae at 2.8 Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of the Family II Inorganic Pyrophosphatase From Streptococcus Agalactiae at 2.8 Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:18.6 occ:1.00 O3 B:2PN1002 2.3 18.4 1.0 O6 B:2PN1002 2.3 18.3 1.0 P1 B:2PN1002 3.5 18.6 1.0 O B:HOH2108 3.5 17.7 1.0 P2 B:2PN1002 3.7 18.3 1.0 O1 B:2PN1002 3.7 18.4 1.0 OE1 B:GLN220 3.9 18.2 1.0 OD2 B:ASP205 3.9 18.5 1.0 N1 B:2PN1002 4.0 17.8 1.0 NH2 B:ARG297 4.1 18.3 1.0 O5 B:2PN1002 4.3 18.0 1.0 O2 B:2PN1002 4.7 18.0 1.0 OD1 B:ASP151 4.7 18.0 1.0 CG B:ASP205 4.8 18.6 1.0 O4 B:2PN1002 4.8 18.6 1.0 O B:HOH2097 4.9 17.7 1.0 OD1 B:ASP205 4.9 18.6 1.0

M.K.Rantanen, L.Lehtio, L.Rajagopal, C.E.Rubens, A.Goldman. Structure of the Streptococcus Agalactiae Family II Inorganic Pyrophosphatase at 2.80 A Resolution Acta Crystallogr.,Sect.D V. 63 738 2007.
ISSN: ISSN 0907-4449
PubMed: 17505113
DOI: 10.1107/S0907444907019695