Magnesium in PDB 2ez6: Crystal Structure of Aquifex Aeolicus Rnase III (D44N) Complexed with Product of Double-Stranded Rna Processing

All present enzymatic activity of Crystal Structure of Aquifex Aeolicus Rnase III (D44N) Complexed with Product of Double-Stranded Rna Processing:
3.1.26.3;

The structure of Crystal Structure of Aquifex Aeolicus Rnase III (D44N) Complexed with Product of Double-Stranded Rna Processing, PDB code: 2ez6 was solved by J.Gan, J.E.Tropea, B.P.Austin, D.L.Court, D.S.Waugh, X.Ji, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.84 / 2.05
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	63.839, 50.976, 113.746, 90.00, 104.47, 90.00
R / Rfree (%)	20.5 / 25.8

The binding sites of Magnesium atom in the Crystal Structure of Aquifex Aeolicus Rnase III (D44N) Complexed with Product of Double-Stranded Rna Processing (pdb code 2ez6). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Aquifex Aeolicus Rnase III (D44N) Complexed with Product of Double-Stranded Rna Processing, PDB code: 2ez6:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Aquifex Aeolicus Rnase III (D44N) Complexed with Product of Double-Stranded Rna Processing


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Aquifex Aeolicus Rnase III (D44N) Complexed with Product of Double-Stranded Rna Processing within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:62.5 occ:1.00 O A:HOH503 2.2 68.6 1.0 OE2 A:GLU40 2.2 60.2 1.0 O C:HOH29 2.3 53.4 1.0 O A:HOH502 2.3 50.7 1.0 OD1 A:ASP107 2.3 46.4 1.0 OE1 A:GLU110 2.3 38.4 1.0 CD A:GLU40 3.2 59.0 1.0 CD A:GLU110 3.3 42.3 1.0 CG A:ASP107 3.5 40.9 1.0 OE2 A:GLU110 3.5 50.0 1.0 O C:HOH87 3.7 51.1 1.0 CG A:GLU40 3.8 40.1 1.0 O C:HOH64 4.0 48.1 1.0 OE1 A:GLU40 4.1 65.9 1.0 OP3 C:A1 4.2 76.4 1.0 OD2 A:ASP107 4.3 47.5 1.0 O A:HOH538 4.3 57.0 1.0 O A:HOH606 4.3 53.5 1.0 CA A:ASP107 4.4 27.6 1.0 CB A:ASP107 4.5 40.9 1.0 OP2 C:A1 4.6 77.6 1.0 CG A:GLU110 4.6 32.7 1.0 O A:HOH579 4.7 58.7 1.0 N A:ASP107 4.8 34.2 1.0 O A:GLY106 4.9 33.8 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Aquifex Aeolicus Rnase III (D44N) Complexed with Product of Double-Stranded Rna Processing


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Aquifex Aeolicus Rnase III (D44N) Complexed with Product of Double-Stranded Rna Processing within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:51.8 occ:1.00 OD1 B:ASP107 2.1 44.6 1.0 O B:HOH503 2.1 62.2 1.0 O D:HOH29 2.2 57.2 1.0 O D:HOH30 2.2 68.0 1.0 OE2 B:GLU40 2.3 65.5 1.0 OE1 B:GLU110 2.3 58.8 1.0 CD B:GLU40 3.2 61.8 1.0 CG B:ASP107 3.3 41.6 1.0 CD B:GLU110 3.4 53.4 1.0 OE2 B:GLU110 3.7 60.2 1.0 O D:HOH34 4.0 52.8 1.0 CG B:GLU40 4.0 48.6 1.0 OE1 B:GLU40 4.0 64.4 1.0 OD2 B:ASP107 4.0 49.1 1.0 CB B:ASP107 4.3 42.8 1.0 OP1 D:A1 4.3 83.7 1.0 CA B:ASP107 4.3 38.6 1.0 OP2 D:A1 4.4 79.5 1.0 CG B:GLU110 4.7 47.6 1.0 N B:ASP107 4.7 37.4 1.0 OD1 B:ASN44 4.9 55.0 1.0 P D:A1 5.0 81.3 1.0

J.Gan, J.E.Tropea, B.P.Austin, D.L.Court, D.S.Waugh, X.Ji. Structural Insight Into the Mechanism of Double-Stranded Rna Processing By Ribonuclease III. Cell(Cambridge,Mass.) V. 124 355 2006.
ISSN: ISSN 0092-8674
PubMed: 16439209
DOI: 10.1016/J.CELL.2005.11.034