Magnesium in PDB 2f1h: Recombinase in Complex with Amp-Pnp and Potassium

The structure of Recombinase in Complex with Amp-Pnp and Potassium, PDB code: 2f1h was solved by X.Qian, Y.He, Y.Wu, Y.Luo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.70
Space group	P 61
Cell size a, b, c (Å), α, β, γ (°)	84.453, 84.453, 105.484, 90.00, 90.00, 120.00
R / Rfree (%)	22.7 / 29.7

The structure of Recombinase in Complex with Amp-Pnp and Potassium also contains other interesting chemical elements:

Potassium

(K)

2 atoms

The binding sites of Magnesium atom in the Recombinase in Complex with Amp-Pnp and Potassium (pdb code 2f1h). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Recombinase in Complex with Amp-Pnp and Potassium, PDB code: 2f1h:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Recombinase in Complex with Amp-Pnp and Potassium


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Recombinase in Complex with Amp-Pnp and Potassium within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:14.0 occ:1.00 O1B A:ANP401 2.2 22.4 1.0 OG1 A:THR112 2.3 20.5 1.0 O2G A:ANP401 2.7 26.8 1.0 O1G A:ANP401 3.0 27.2 1.0 PG A:ANP401 3.3 24.4 1.0 PB A:ANP401 3.5 23.8 1.0 CB A:THR112 3.6 18.8 1.0 K A:K504 3.8 40.4 1.0 N3B A:ANP401 3.8 25.1 1.0 OD2 A:ASP151 3.9 25.6 1.0 OD2 A:ASP211 4.2 19.2 1.0 OD1 A:ASP211 4.2 23.9 1.0 OG1 A:THR153 4.2 19.1 1.0 O3A A:ANP401 4.3 26.1 1.0 CG2 A:THR112 4.4 19.7 1.0 N A:THR112 4.5 19.8 1.0 O2B A:ANP401 4.5 22.9 1.0 CA A:THR112 4.5 19.2 1.0 O2A A:ANP401 4.6 27.6 1.0 CG A:ASP211 4.6 21.3 1.0 O3G A:ANP401 4.6 26.4 1.0 O1A A:ANP401 4.8 27.1 1.0 CB A:THR153 4.8 18.3 1.0 CG2 A:THR153 4.8 18.2 1.0 PA A:ANP401 4.9 27.4 1.0 CG A:ASP151 5.0 24.3 1.0

Magnesium binding site 2 out of 2 in the Recombinase in Complex with Amp-Pnp and Potassium


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Recombinase in Complex with Amp-Pnp and Potassium within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:17.8 occ:1.00 O A:GLN98 1.8 27.7 1.0 OD1 A:ASP246 2.0 20.2 1.0 CG A:ASP246 3.0 21.9 1.0 C A:GLN98 3.0 26.9 1.0 OD2 A:ASP246 3.3 22.0 1.0 ND2 A:ASN242 3.5 25.8 1.0 N A:SER99 3.8 27.5 1.0 CA A:SER99 3.8 26.8 1.0 CA A:GLN98 4.1 26.2 1.0 OG A:SER99 4.2 29.4 1.0 O A:ASN242 4.3 23.4 1.0 CB A:ASP246 4.4 21.3 1.0 CB A:GLN98 4.4 23.8 1.0 CB A:SER99 4.5 27.4 1.0 CG A:ASN242 4.6 24.8 1.0 N A:ASP246 4.6 24.0 1.0 CA A:ASP246 4.7 23.9 1.0 CB A:ALA245 4.9 21.1 1.0 C A:SER99 4.9 25.1 1.0

X.Qian, Y.He, Y.Wu, Y.Luo. ASP302 Determines Potassium Dependence of A Rada Recombinase From Methanococcus Voltae. J.Mol.Biol. V. 360 537 2006.
ISSN: ISSN 0022-2836
PubMed: 16782126
DOI: 10.1016/J.JMB.2006.05.058