Magnesium in PDB 2f7t: Crystal Structure of the Catalytic Domain of MOS1 Mariner Transposase

The structure of Crystal Structure of the Catalytic Domain of MOS1 Mariner Transposase, PDB code: 2f7t was solved by J.M.Richardson, A.Dawson, P.Taylor, D.J.Finnegan, M.D.Walkinshaw, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.25
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	44.243, 44.243, 206.047, 90.00, 90.00, 90.00
R / Rfree (%)	18.9 / 27.7

The binding sites of Magnesium atom in the Crystal Structure of the Catalytic Domain of MOS1 Mariner Transposase (pdb code 2f7t). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of the Catalytic Domain of MOS1 Mariner Transposase, PDB code: 2f7t:

Magnesium binding site 1 out of 1 in the Crystal Structure of the Catalytic Domain of MOS1 Mariner Transposase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Catalytic Domain of MOS1 Mariner Transposase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1745 b:31.6 occ:1.00 O A:HOH1066 2.0 25.2 1.0 OD2 A:ASP249 2.1 28.6 1.0 O A:HOH1070 2.1 34.2 1.0 O A:HOH1067 2.1 28.4 1.0 OD1 A:ASP156 2.2 19.4 1.0 O A:HOH1059 2.2 39.5 1.0 CG A:ASP249 3.1 29.6 1.0 CG A:ASP156 3.2 23.1 1.0 OD2 A:ASP156 3.4 24.0 1.0 OD1 A:ASP249 3.4 25.9 1.0 O A:HOH1019 3.9 28.8 1.0 OE2 A:GLU157 3.9 38.2 1.0 O A:HOH1004 4.0 22.9 1.0 CB A:ASP249 4.4 27.5 1.0 N A:GLU157 4.6 22.4 1.0 CB A:ASP156 4.6 21.1 1.0 O A:GLU157 4.7 26.2 1.0 O A:HOH1042 4.9 43.6 1.0 CA A:ASP156 4.9 21.0 1.0

J.M.Richardson, A.Dawson, N.O'hagan, P.Taylor, D.J.Finnegan, M.D.Walkinshaw. Mechanism of MOS1 Transposition: Insights From Structural Analysis Embo J. V. 25 1324 2006.
ISSN: ISSN 0261-4189
PubMed: 16511570
DOI: 10.1038/SJ.EMBOJ.7601018