Magnesium in PDB 2f96: 2.1 A Crystal Structure of Pseudomonas Aeruginosa Rnase T (Ribonuclease T)

The structure of 2.1 A Crystal Structure of Pseudomonas Aeruginosa Rnase T (Ribonuclease T), PDB code: 2f96 was solved by H.Zheng, M.Chruszcz, M.Cymborowski, Y.Wang, E.Gorodichtchenskaia, T.Skarina, J.Guthrie, A.Savchenko, A.Edwards, A.Joachimiak, W.Minor, Midwest Center For Structural Genomics (Mcsg), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.97 / 2.09
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	49.879, 76.636, 61.666, 90.00, 93.55, 90.00
R / Rfree (%)	15.8 / 20.4

The binding sites of Magnesium atom in the 2.1 A Crystal Structure of Pseudomonas Aeruginosa Rnase T (Ribonuclease T) (pdb code 2f96). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the 2.1 A Crystal Structure of Pseudomonas Aeruginosa Rnase T (Ribonuclease T), PDB code: 2f96:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the 2.1 A Crystal Structure of Pseudomonas Aeruginosa Rnase T (Ribonuclease T)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of 2.1 A Crystal Structure of Pseudomonas Aeruginosa Rnase T (Ribonuclease T) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg2001 b:36.4 occ:1.00 O A:HOH2100 2.0 37.2 1.0 O A:HOH2098 2.0 35.8 1.0 OD1 A:ASP35 2.1 38.7 1.0 O A:HOH2099 2.2 46.6 1.0 O A:HOH2048 2.3 28.2 1.0 O A:HOH2103 2.5 47.6 1.0 CG A:ASP35 3.1 37.4 1.0 OD2 A:ASP35 3.4 42.3 1.0 O A:HOH2076 3.9 43.9 1.0 O A:HOH2017 3.9 25.7 1.0 OD1 A:ASP137 4.1 31.1 1.0 O A:HOH2052 4.1 40.1 1.0 OD2 A:ASP198 4.3 51.8 1.0 O A:HIS132 4.3 25.7 1.0 O A:VAL36 4.5 23.0 1.0 OD2 A:ASP137 4.5 35.5 1.0 CB A:ASP35 4.5 23.9 1.0 O A:HOH2071 4.6 45.2 1.0 CG A:ASP137 4.7 32.9 1.0

Magnesium binding site 2 out of 2 in the 2.1 A Crystal Structure of Pseudomonas Aeruginosa Rnase T (Ribonuclease T)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of 2.1 A Crystal Structure of Pseudomonas Aeruginosa Rnase T (Ribonuclease T) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1001 b:35.9 occ:1.00 O B:HOH1119 1.9 31.8 1.0 O B:HOH1095 2.0 40.1 1.0 OD1 B:ASP35 2.1 42.3 1.0 O B:HOH1120 2.1 25.2 1.0 O B:HOH1084 2.1 44.3 1.0 O B:HOH1109 2.2 35.0 1.0 CG B:ASP35 3.1 36.3 1.0 OD2 B:ASP35 3.5 42.8 1.0 OD1 B:ASP137 3.6 33.6 1.0 O B:HOH1015 3.8 25.9 1.0 O B:HOH1121 4.0 43.9 1.0 O B:HOH1103 4.0 39.8 1.0 O B:HOH1055 4.2 35.3 1.0 OD2 B:ASP137 4.2 34.1 1.0 O B:VAL36 4.3 25.2 1.0 O B:HIS132 4.3 26.6 1.0 O B:HOH1049 4.3 42.9 1.0 CG B:ASP137 4.4 32.2 1.0 OD2 B:ASP198 4.4 54.5 1.0 CB B:ASP35 4.4 27.9 1.0 CE1 B:HIS193 4.8 61.5 1.0 CA B:ASP35 4.9 26.8 1.0 N B:VAL36 4.9 25.7 1.0 NE2 B:HIS193 4.9 61.9 1.0

Y.Zuo, H.Zheng, Y.Wang, M.Chruszcz, M.Cymborowski, T.Skarina, A.Savchenko, A.Malhotra, W.Minor. Crystal Structure of Rnase T, An Exoribonuclease Involved in Trna Maturation and End Turnover. Structure V. 15 417 2007.
ISSN: ISSN 0969-2126
PubMed: 17437714
DOI: 10.1016/J.STR.2007.02.004