Magnesium in PDB 2g83: Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit

The structure of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit, PDB code: 2g83 was solved by C.A.Johnston, J.K.Ramer, R.Blaesius, B.Kuhlman, V.Y.Arshavsky, D.P.Siderovski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	103.130, 103.130, 206.990, 90.00, 90.00, 120.00
R / Rfree (%)	26.9 / 30

The structure of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit also contains other interesting chemical elements:

Fluorine	(F)	8 atoms
Aluminium	(Al)	2 atoms

The binding sites of Magnesium atom in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit (pdb code 2g83). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit, PDB code: 2g83:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg356 b:29.3 occ:1.00 O3B A:GDP355 1.5 34.7 1.0 OG1 A:THR181 1.7 42.1 1.0 OG A:SER47 1.9 26.1 1.0 F1 A:ALF357 2.6 55.6 1.0 PB A:GDP355 2.8 39.4 1.0 F4 A:ALF357 3.0 54.9 1.0 CB A:SER47 3.0 27.2 1.0 CB A:THR181 3.1 42.6 1.0 O A:HOH2 3.3 44.1 1.0 O1B A:GDP355 3.4 39.6 1.0 NH1 A:ARG178 3.6 37.5 1.0 O A:VAL179 3.6 41.8 1.0 O3A A:GDP355 3.6 39.7 1.0 N A:THR181 3.6 37.6 1.0 O2A A:GDP355 3.7 40.0 1.0 CG2 A:THR181 3.8 42.9 1.0 AL A:ALF357 3.8 56.2 1.0 CA A:THR181 3.9 37.7 1.0 O2B A:GDP355 3.9 37.6 1.0 PA A:GDP355 4.1 37.4 1.0 F2 A:ALF357 4.3 53.6 1.0 CA A:SER47 4.3 42.1 1.0 C A:LYS180 4.4 30.5 1.0 O1A A:GDP355 4.4 38.3 1.0 F3 A:ALF357 4.5 53.9 1.0 N A:SER47 4.5 43.3 1.0 CA A:LYS180 4.5 30.3 1.0 C A:VAL179 4.6 40.7 1.0 CZ A:ARG178 4.7 36.9 1.0

Magnesium binding site 2 out of 2 in the Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Activated G-Alpha-I1 Bound to A Nucleotide- State-Selective Peptide: Minimal Determinants For Recognizing the Active Form of A G Protein Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg358 b:45.1 occ:1.00 OG1 B:THR181 1.7 41.8 1.0 OG B:SER47 1.8 41.2 1.0 O3B B:GDP355 2.5 39.1 1.0 O1B B:GDP355 2.8 44.3 1.0 CB B:SER47 3.0 42.1 1.0 PB B:GDP355 3.1 42.4 1.0 CB B:THR181 3.1 41.7 1.0 F3 B:ALF359 3.2 63.7 1.0 O B:HOH1 3.2 35.6 1.0 AL B:ALF359 3.3 61.6 1.0 N B:THR181 3.4 46.8 1.0 O B:VAL179 3.6 44.0 1.0 F2 B:ALF359 3.6 59.9 1.0 CA B:THR181 3.8 44.8 1.0 O3A B:GDP355 3.8 45.5 1.0 NH1 B:ARG178 3.9 54.3 1.0 CG2 B:THR181 3.9 39.5 1.0 O2A B:GDP355 4.0 42.9 1.0 PA B:GDP355 4.0 45.0 1.0 C B:LYS180 4.2 46.6 1.0 CA B:SER47 4.3 41.0 1.0 O2B B:GDP355 4.4 38.5 1.0 N B:SER47 4.4 41.3 1.0 CA B:LYS180 4.4 47.2 1.0 F4 B:ALF359 4.5 65.1 1.0 C B:VAL179 4.5 43.6 1.0 O1A B:GDP355 4.7 44.5 1.0 CZ B:ARG178 4.8 54.5 1.0 F1 B:ALF359 4.9 62.9 1.0 N B:LYS180 4.9 47.5 1.0

C.A.Johnston, E.S.Lobanova, A.S.Shavkunov, J.Low, J.K.Ramer, R.Blaesius, Z.Fredericks, F.S.Willard, B.Kuhlman, V.Y.Arshavsky, D.P.Siderovski. Minimal Determinants For Binding Activated Galpha From the Structure of A Galpha(I1)-Peptide Dimer. Biochemistry V. 45 11390 2006.
ISSN: ISSN 0006-2960
PubMed: 16981699
DOI: 10.1021/BI0613832