Magnesium in PDB 2ghq: Ctd-Specific Phosphatase SCP1 in Complex with Peptide C- Terminal Domain of Rna Polymerase II

All present enzymatic activity of Ctd-Specific Phosphatase SCP1 in Complex with Peptide C- Terminal Domain of Rna Polymerase II:
2.7.7.6; 3.1.3.16;

The structure of Ctd-Specific Phosphatase SCP1 in Complex with Peptide C- Terminal Domain of Rna Polymerase II, PDB code: 2ghq was solved by Y.Zhang, J.P.Noel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.98 / 2.05
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	126.067, 78.813, 62.992, 90.00, 112.17, 90.00
R / Rfree (%)	21.8 / 24.8

The binding sites of Magnesium atom in the Ctd-Specific Phosphatase SCP1 in Complex with Peptide C- Terminal Domain of Rna Polymerase II (pdb code 2ghq). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Ctd-Specific Phosphatase SCP1 in Complex with Peptide C- Terminal Domain of Rna Polymerase II, PDB code: 2ghq:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Ctd-Specific Phosphatase SCP1 in Complex with Peptide C- Terminal Domain of Rna Polymerase II


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Ctd-Specific Phosphatase SCP1 in Complex with Peptide C- Terminal Domain of Rna Polymerase II within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg257 b:42.6 occ:1.00 O2P D:SEP174 2.1 32.0 1.0 OD1 B:ASN207 2.3 28.4 1.0 OD1 B:ASN96 2.3 30.0 1.0 O B:ASP98 2.3 25.1 1.0 CG B:ASN207 3.1 26.6 1.0 ND2 B:ASN207 3.3 27.1 1.0 CG B:ASN96 3.4 29.7 1.0 P D:SEP174 3.5 32.6 1.0 C B:ASP98 3.5 28.5 1.0 ND2 B:ASN96 3.8 28.0 1.0 OG D:SER176 3.8 61.1 1.0 O3P D:SEP174 4.0 35.6 1.0 OD1 B:ASP206 4.0 37.3 1.0 CB B:GLU99 4.1 30.1 1.0 O1P D:SEP174 4.1 34.6 1.0 OE1 B:GLU99 4.1 34.8 1.0 OG1 B:THR100 4.2 26.1 1.0 CA B:ASP98 4.3 29.1 1.0 N B:ASP98 4.3 28.0 1.0 CB B:ASP98 4.5 32.3 1.0 OG B:SER208 4.5 38.6 1.0 N B:GLU99 4.5 27.4 1.0 CB D:SEP174 4.6 40.0 1.0 CB B:ASN207 4.6 26.8 1.0 CB B:ASN96 4.6 27.9 1.0 OG D:SEP174 4.7 37.6 1.0 CA B:GLU99 4.7 28.1 1.0 CD B:GLU99 4.8 35.1 1.0 CG B:ASP206 4.8 36.5 1.0 N B:ASN207 4.8 27.2 1.0 N B:THR100 4.8 25.3 1.0 CG B:GLU99 4.9 31.7 1.0 OD2 B:ASP206 4.9 38.9 1.0 C B:GLU99 4.9 27.4 1.0

Magnesium binding site 2 out of 2 in the Ctd-Specific Phosphatase SCP1 in Complex with Peptide C- Terminal Domain of Rna Polymerase II


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Ctd-Specific Phosphatase SCP1 in Complex with Peptide C- Terminal Domain of Rna Polymerase II within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg151 b:44.3 occ:1.00 O1P C:SEP174 1.6 38.2 1.0 O A:ASP98 2.1 30.5 1.0 OD1 A:ASN96 2.2 33.2 1.0 OD1 A:ASN207 2.6 34.8 1.0 P C:SEP174 3.0 36.9 1.0 CG A:ASN96 3.2 31.5 1.0 C A:ASP98 3.2 32.5 1.0 ND2 A:ASN96 3.5 29.3 1.0 O3P C:SEP174 3.6 38.4 1.0 CG A:ASN207 3.6 35.4 1.0 O2P C:SEP174 3.6 37.4 1.0 CA A:ASP98 3.8 31.9 1.0 O C:HOH96 3.9 52.6 1.0 ND2 A:ASN207 3.9 32.8 1.0 CB A:ASP98 3.9 35.5 1.0 N A:ASP98 4.0 28.6 1.0 O C:HOH99 4.0 48.3 1.0 OE1 A:GLU99 4.1 32.6 1.0 OD1 A:ASP206 4.2 38.3 1.0 OG C:SEP174 4.3 39.7 1.0 OG1 A:THR100 4.3 29.0 1.0 CB A:GLU99 4.3 32.4 1.0 N A:GLU99 4.4 31.1 1.0 CB C:SEP174 4.4 41.9 1.0 CB A:ASN96 4.5 30.8 1.0 C A:LEU97 4.7 27.2 1.0 CA A:GLU99 4.8 32.7 1.0 N A:THR100 4.8 30.3 1.0 CD A:GLU99 4.9 35.6 1.0 CB A:ASN207 4.9 36.0 1.0 N A:LEU97 5.0 26.6 1.0

Y.Zhang, Y.Kim, N.Genoud, J.Gao, J.W.Kelly, S.L.Pfaff, G.N.Gill, J.E.Dixon, J.P.Noel. Determinants For Dephosphorylation of the Rna Polymerase II C-Terminal Domain By SCP1. Mol.Cell V. 24 759 2006.
ISSN: ISSN 1097-2765
PubMed: 17157258
DOI: 10.1016/J.MOLCEL.2006.10.027