Magnesium in PDB 2hcj: Trypsin-Modified Elongation Factor Tu in Complex with Tetracycline

The structure of Trypsin-Modified Elongation Factor Tu in Complex with Tetracycline, PDB code: 2hcj was solved by S.Mui, S.E.Heffron, A.Aorora, K.Abel, E.Bergmann, F.Jurnak, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	51.92 / 2.12
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	69.110, 69.110, 157.330, 90.00, 90.00, 90.00
R / Rfree (%)	20 / 23.4

The structure of Trypsin-Modified Elongation Factor Tu in Complex with Tetracycline also contains other interesting chemical elements:

Sodium

(Na)

3 atoms

The binding sites of Magnesium atom in the Trypsin-Modified Elongation Factor Tu in Complex with Tetracycline (pdb code 2hcj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Trypsin-Modified Elongation Factor Tu in Complex with Tetracycline, PDB code: 2hcj:

Magnesium binding site 1 out of 1 in the Trypsin-Modified Elongation Factor Tu in Complex with Tetracycline


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Trypsin-Modified Elongation Factor Tu in Complex with Tetracycline within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg998 b:22.3 occ:1.00 O12 B:TAC888 1.9 21.8 1.0 O A:HOH2001 2.0 21.2 1.0 O B:HOH2002 2.0 27.7 1.0 O2B A:GDP999 2.1 22.3 1.0 O11 B:TAC888 2.1 22.8 1.0 OG1 A:THR25 2.2 22.0 1.0 C12 B:TAC888 3.1 27.5 1.0 C11 B:TAC888 3.1 28.0 1.0 PB A:GDP999 3.2 27.0 1.0 CB A:THR25 3.3 27.2 1.0 O1B A:GDP999 3.3 28.9 1.0 C1B B:TAC888 3.6 30.7 1.0 N A:THR25 4.0 22.6 1.0 O B:CSO81 4.0 26.1 1.0 OD2 B:ASP80 4.0 29.4 1.0 O B:HOH2004 4.0 28.1 1.0 O3B A:GDP999 4.1 23.7 1.0 CA A:THR25 4.2 23.5 1.0 O10 B:TAC888 4.2 24.2 1.0 O B:HOH2003 4.3 27.1 1.0 O B:PRO82 4.3 28.2 1.0 CG2 A:THR25 4.4 22.5 1.0 O2A A:GDP999 4.4 28.1 1.0 C1C B:TAC888 4.4 30.5 1.0 C1A B:TAC888 4.4 21.0 1.0 O3A A:GDP999 4.4 27.6 1.0 OD1 B:ASP80 4.5 26.4 1.0 O1C B:TAC888 4.5 26.6 1.0 CE A:LYS24 4.6 22.5 1.0 O1 B:TAC888 4.6 35.9 1.0 CA B:PRO82 4.6 25.6 1.0 CG B:ASP80 4.6 23.6 1.0 NZ A:LYS24 4.8 22.1 1.0 PA A:GDP999 4.8 28.4 1.0 CB A:LYS24 4.8 27.7 1.0 C B:CSO81 4.8 25.1 1.0 O1A A:GDP999 4.8 31.5 1.0 C10 B:TAC888 4.8 24.4 1.0 C A:LYS24 4.9 23.6 1.0 C B:PRO82 5.0 27.2 1.0

S.E.Heffron, S.Mui, A.Aorora, K.Abel, E.Bergmann, F.Jurnak. Molecular Complementarity Between Tetracycline and the Gtpase Active Site of Elongation Factor Tu. Acta Crystallogr.,Sect.D V. 62 1392 2006.
ISSN: ISSN 0907-4449
PubMed: 17057344
DOI: 10.1107/S0907444906035426