Magnesium in PDB 2hht: C:O6-Methyl-Guanine Pair in the Polymerase-2 Basepair Position

All present enzymatic activity of C:O6-Methyl-Guanine Pair in the Polymerase-2 Basepair Position:
2.7.7.7;

The structure of C:O6-Methyl-Guanine Pair in the Polymerase-2 Basepair Position, PDB code: 2hht was solved by J.J.Warren, L.J.Forsberg, L.S.Beese, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.05
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	87.864, 93.431, 107.071, 90.00, 90.00, 90.00
R / Rfree (%)	19.7 / 22.9

The binding sites of Magnesium atom in the C:O6-Methyl-Guanine Pair in the Polymerase-2 Basepair Position (pdb code 2hht). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the C:O6-Methyl-Guanine Pair in the Polymerase-2 Basepair Position, PDB code: 2hht:

Magnesium binding site 1 out of 1 in the C:O6-Methyl-Guanine Pair in the Polymerase-2 Basepair Position


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of C:O6-Methyl-Guanine Pair in the Polymerase-2 Basepair Position within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg920 b:30.6 occ:1.00 OD1 A:ASP653 2.8 20.4 1.0 O A:TYR654 2.8 14.8 1.0 OD2 A:ASP830 2.8 20.5 1.0 O A:HOH2525 3.0 20.1 1.0 O A:HOH2512 3.7 15.6 1.0 O A:HOH2934 3.7 40.3 1.0 CG A:ASP653 3.8 18.6 1.0 CG A:ASP830 3.8 20.0 1.0 O A:HOH3302 3.8 42.1 1.0 C A:TYR654 4.0 14.2 1.0 CB A:ASP653 4.0 16.7 1.0 O A:HOH2845 4.0 23.9 1.0 OD1 A:ASP830 4.2 19.5 1.0 O A:HOH2645 4.6 30.5 1.0 CA A:SER655 4.6 15.1 1.0 N A:TYR654 4.6 14.5 1.0 C A:ASP653 4.7 14.6 1.0 N A:SER655 4.8 13.6 1.0 O A:HOH3299 4.8 47.8 1.0 OD2 A:ASP653 4.9 20.2 1.0 N A:GLN656 4.9 13.9 1.0 CB A:ASP830 5.0 17.8 1.0 CA A:TYR654 5.0 12.8 1.0 O A:ASP830 5.0 14.6 1.0

J.J.Warren, L.J.Forsberg, L.S.Beese. The Structural Basis For the Mutagenicity of O6-Methyl-Guanine Lesions. Proc.Natl.Acad.Sci.Usa V. 103 19701 2006.
ISSN: ISSN 0027-8424
PubMed: 17179038
DOI: 10.1073/PNAS.0609580103