Magnesium in PDB 2hw3: T:O6-Methyl-Guanine Pair in the Polymerase Postinsertion Site (-1 Basepair Position)

All present enzymatic activity of T:O6-Methyl-Guanine Pair in the Polymerase Postinsertion Site (-1 Basepair Position):
2.7.7.7;

The structure of T:O6-Methyl-Guanine Pair in the Polymerase Postinsertion Site (-1 Basepair Position), PDB code: 2hw3 was solved by J.J.Warren, L.J.Forsberg, L.S.Beese, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.88 / 1.98
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	88.098, 93.684, 105.250, 90.00, 90.00, 90.00
R / Rfree (%)	21 / 25.2

The binding sites of Magnesium atom in the T:O6-Methyl-Guanine Pair in the Polymerase Postinsertion Site (-1 Basepair Position) (pdb code 2hw3). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the T:O6-Methyl-Guanine Pair in the Polymerase Postinsertion Site (-1 Basepair Position), PDB code: 2hw3:

Magnesium binding site 1 out of 1 in the T:O6-Methyl-Guanine Pair in the Polymerase Postinsertion Site (-1 Basepair Position)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of T:O6-Methyl-Guanine Pair in the Polymerase Postinsertion Site (-1 Basepair Position) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg906 b:32.7 occ:1.00 O A:TYR654 2.7 12.7 1.0 OD2 A:ASP830 2.7 19.7 1.0 O A:HOH1041 2.8 20.3 1.0 OD2 A:ASP653 3.0 22.1 1.0 O A:HOH1072 3.4 16.8 1.0 O A:HOH1356 3.4 36.2 1.0 CG A:ASP830 3.6 19.3 1.0 C A:TYR654 3.8 13.1 1.0 CG A:ASP653 3.9 18.5 1.0 O A:HOH1283 4.0 29.7 1.0 OD1 A:ASP830 4.0 20.5 1.0 CB A:ASP653 4.1 15.8 1.0 O A:HOH1024 4.2 23.7 1.0 O A:HOH1432 4.4 44.4 1.0 CA A:SER655 4.5 13.8 1.0 O A:HOH1082 4.5 38.5 1.0 N A:SER655 4.6 13.2 1.0 N A:TYR654 4.6 13.5 1.0 C A:ASP653 4.8 14.2 1.0 N A:GLN656 4.8 13.5 1.0 CB A:ASP830 4.8 18.2 1.0 CA A:TYR654 4.9 13.0 1.0

J.J.Warren, L.J.Forsberg, L.S.Beese. The Structural Basis For the Mutagenicity of O6-Methyl-Guanine Lesions. Proc.Natl.Acad.Sci.Usa V. 103 19701 2006.
ISSN: ISSN 0027-8424
PubMed: 17179038
DOI: 10.1073/PNAS.0609580103