Magnesium in PDB 2i54: Phosphomannomutase From Leishmania Mexicana

All present enzymatic activity of Phosphomannomutase From Leishmania Mexicana:
5.4.2.8;

The structure of Phosphomannomutase From Leishmania Mexicana, PDB code: 2i54 was solved by B.J.Smith, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.80 / 2.10
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	92.413, 92.413, 173.249, 90.00, 90.00, 120.00
R / Rfree (%)	18.9 / 23

The structure of Phosphomannomutase From Leishmania Mexicana also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Magnesium atom in the Phosphomannomutase From Leishmania Mexicana (pdb code 2i54). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Phosphomannomutase From Leishmania Mexicana, PDB code: 2i54:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Phosphomannomutase From Leishmania Mexicana


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Phosphomannomutase From Leishmania Mexicana within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg2002 b:70.1 occ:1.00 OD2 A:ASP10 2.2 53.0 1.0 OD2 A:ASP207 2.4 53.9 1.0 O A:ASP12 2.6 52.5 1.0 CG A:ASP10 3.3 49.9 1.0 CG A:ASP207 3.3 49.0 1.0 OD1 A:ASP207 3.3 49.3 1.0 OD1 A:ASP10 3.6 55.2 1.0 C A:ASP12 3.7 51.4 1.0 OD2 A:ASP215 4.0 48.3 1.0 OG1 A:THR14 4.4 50.6 1.0 CB A:LYS208 4.5 50.5 1.0 CB A:ASP12 4.5 51.6 1.0 CA A:ASP12 4.5 51.3 1.0 N A:GLY13 4.6 51.2 1.0 CB A:ASP10 4.6 49.6 1.0 ND2 A:ASN214 4.6 50.8 1.0 CA A:GLY13 4.7 51.0 1.0 N A:ASP12 4.7 51.4 1.0 CB A:ASP207 4.7 49.6 1.0 O A:HOH3026 4.9 47.9 1.0 CG A:ASP215 4.9 49.0 1.0 N A:THR14 4.9 50.6 1.0 N A:ASP207 5.0 49.0 1.0

Magnesium binding site 2 out of 3 in the Phosphomannomutase From Leishmania Mexicana


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Phosphomannomutase From Leishmania Mexicana within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2001 b:63.8 occ:1.00 O B:ASP12 2.2 52.6 1.0 OD2 B:ASP207 2.4 50.9 1.0 OD2 B:ASP10 2.5 53.8 1.0 CG B:ASP10 3.2 50.1 1.0 CG B:ASP207 3.4 46.9 1.0 C B:ASP12 3.4 51.9 1.0 OD1 B:ASP207 3.6 47.9 1.0 OD1 B:ASP10 3.6 51.1 1.0 OG1 B:THR14 4.0 48.9 1.0 OD2 B:ASP215 4.0 49.1 1.0 CB B:ASP10 4.1 48.7 1.0 N B:ASP12 4.2 51.7 1.0 CA B:ASP12 4.2 52.1 1.0 N B:GLY13 4.4 51.2 1.0 CG B:LYS208 4.5 50.5 1.0 CB B:ASP12 4.5 52.8 1.0 CA B:GLY13 4.5 51.5 1.0 N B:THR14 4.7 49.3 1.0 C B:VAL11 4.7 51.3 1.0 O B:HOH3027 4.7 33.2 1.0 N B:ASP207 4.7 45.8 1.0 C B:GLY13 4.7 51.0 1.0 CB B:ASP207 4.8 46.6 1.0 OD1 B:ASP12 4.8 58.6 1.0 N B:VAL11 4.8 48.8 1.0 CB B:THR14 4.9 49.4 1.0 OD1 B:ASN214 5.0 49.3 1.0

Magnesium binding site 3 out of 3 in the Phosphomannomutase From Leishmania Mexicana


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Phosphomannomutase From Leishmania Mexicana within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg2003 b:74.9 occ:1.00 OD2 C:ASP207 2.1 55.7 1.0 O C:ASP12 2.3 51.7 1.0 O C:HOH3030 2.7 59.3 1.0 OD2 C:ASP10 2.9 52.8 1.0 CG C:ASP207 3.1 51.2 1.0 OD1 C:ASP207 3.3 54.0 1.0 C C:ASP12 3.5 51.0 1.0 CG C:ASP10 3.5 50.4 1.0 OD1 C:ASP10 3.9 52.8 1.0 OG1 C:THR14 4.1 45.5 1.0 OD2 C:ASP215 4.1 50.3 1.0 N C:GLY13 4.4 50.2 1.0 CA C:GLY13 4.4 50.2 1.0 CA C:ASP12 4.5 51.6 1.0 CG C:LYS208 4.5 50.1 1.0 N C:ASP12 4.5 51.4 1.0 CB C:ASP207 4.5 48.9 1.0 CB C:ASP10 4.5 49.1 1.0 N C:ASP207 4.7 46.9 1.0 CB C:ASP12 4.8 52.7 1.0 N C:THR14 4.8 48.9 1.0 C C:GLY13 4.8 49.5 1.0 OD2 C:ASP12 4.9 60.8 1.0 N C:LYS208 5.0 48.7 1.0

L.Kedzierski, R.L.Malby, B.J.Smith, M.A.Perugini, A.N.Hodder, T.Ilg, P.M.Colman, E.Handman. Structure of Leishmania Mexicana Phosphomannomutase Highlights Similarities with Human Isoforms J.Mol.Biol. V. 363 215 2006.
ISSN: ISSN 0022-2836
PubMed: 16963079
DOI: 10.1016/J.JMB.2006.08.023