Magnesium in PDB 2j7n: Structure of the Rnai Polymerase From Neurospora Crassa

The structure of Structure of the Rnai Polymerase From Neurospora Crassa, PDB code: 2j7n was solved by P.S.Salgado, M.R.L.Koivunen, E.V.Makeyev, D.H.Bamford, D.I.Stuart, J.M.Grimes, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.98 / 2.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	101.021, 122.553, 114.701, 90.00, 108.90, 90.00
R / Rfree (%)	21.7 / 26.4

The binding sites of Magnesium atom in the Structure of the Rnai Polymerase From Neurospora Crassa (pdb code 2j7n). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of the Rnai Polymerase From Neurospora Crassa, PDB code: 2j7n:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structure of the Rnai Polymerase From Neurospora Crassa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of the Rnai Polymerase From Neurospora Crassa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg3374 b:50.7 occ:1.00 O A:HOH2340 2.0 32.3 1.0 OD1 A:ASP1007 2.1 49.0 1.0 OD1 A:ASP1009 2.1 51.4 1.0 O A:HOH2342 2.1 34.2 1.0 OD1 A:ASP1011 2.2 52.6 1.0 CG A:ASP1009 2.9 50.0 1.0 CG A:ASP1007 3.1 49.4 1.0 CG A:ASP1011 3.1 51.4 1.0 OD2 A:ASP1009 3.2 52.5 1.0 OD2 A:ASP1011 3.4 51.7 1.0 OD2 A:ASP1007 3.4 52.5 1.0 O A:HOH2336 3.8 29.7 1.0 O A:HOH2343 4.0 47.6 1.0 CB A:ASP1009 4.2 48.1 1.0 N A:ASP1009 4.4 47.4 1.0 N A:ASP1011 4.4 50.2 1.0 CB A:ASP1007 4.5 48.4 1.0 CB A:ASP1011 4.5 50.6 1.0 N A:ASP1007 4.5 48.8 1.0 CA A:ASP1009 4.6 47.9 1.0 O A:ASP1007 4.6 47.8 1.0 C A:ASP1007 4.7 47.9 1.0 C A:ASP1009 4.7 48.3 1.0 CA A:ASP1007 4.8 48.3 1.0 N A:GLY1010 4.9 48.5 1.0 CA A:ASP1011 4.9 50.4 1.0

Magnesium binding site 2 out of 2 in the Structure of the Rnai Polymerase From Neurospora Crassa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of the Rnai Polymerase From Neurospora Crassa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg3374 b:31.6 occ:1.00 OD1 B:ASP1011 1.9 51.4 1.0 OD1 B:ASP1009 2.0 52.0 1.0 OD1 B:ASP1007 2.0 47.1 1.0 O B:HOH2279 2.1 30.4 1.0 O B:HOH2275 2.1 33.0 1.0 O B:HOH2276 2.1 34.0 1.0 CG B:ASP1011 2.9 49.1 1.0 CG B:ASP1009 3.1 49.2 1.0 CG B:ASP1007 3.1 47.9 1.0 OD2 B:ASP1011 3.3 48.3 1.0 OD2 B:ASP1007 3.5 48.5 1.0 OD2 B:ASP1009 3.5 51.0 1.0 O B:HOH2277 3.6 56.4 1.0 O B:HOH2271 4.2 36.3 1.0 N B:ASP1011 4.3 47.8 1.0 N B:ASP1009 4.3 46.9 1.0 CB B:ASP1011 4.3 48.0 1.0 CB B:ASP1009 4.3 47.1 1.0 N B:ASP1007 4.4 48.2 1.0 CB B:ASP1007 4.4 48.5 1.0 C B:ASP1007 4.6 48.7 1.0 CA B:ASP1009 4.6 47.5 1.0 C B:ASP1009 4.6 47.7 1.0 O B:ASP1007 4.7 49.3 1.0 CA B:ASP1007 4.7 48.7 1.0 CA B:ASP1011 4.7 47.7 1.0 N B:GLY1010 4.9 48.0 1.0 O B:ASP1009 5.0 48.8 1.0

P.S.Salgado, M.R.L.Koivunen, E.V.Makeyev, D.H.Bamford, D.I.Stuart, J.M.Grimes. The Structure of An Rnai Polymerase Links Rna Silencing and Transcription. Plos Biol. V. 4 E434 2006.
ISSN: ISSN 1544-9173
PubMed: 17147473
DOI: 10.1371/JOURNAL.PBIO.0040434