Magnesium in PDB 2ode: Crystal Structure of the Heterodimeric Complex of Human RGS8 and Activated Gi Alpha 3

The structure of Crystal Structure of the Heterodimeric Complex of Human RGS8 and Activated Gi Alpha 3, PDB code: 2ode was solved by C.Gileadi, M.Soundararajan, A.P.Turnbull, J.M.Elkins, E.Papagrigoriou, A.C.W.Pike, G.Bunkoczi, F.Gorrec, C.Umeano, F.Von Delft, J.Weigelt, A.Edwards, C.H.Arrowsmith, M.Sundstrom, D.A.Doyle, Structural Genomicsconsortium (Sgc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.40 / 1.90
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	112.837, 130.216, 68.519, 90.00, 90.00, 90.00
R / Rfree (%)	18 / 21.1

The structure of Crystal Structure of the Heterodimeric Complex of Human RGS8 and Activated Gi Alpha 3 also contains other interesting chemical elements:

Fluorine	(F)	8 atoms
Aluminium	(Al)	2 atoms

The binding sites of Magnesium atom in the Crystal Structure of the Heterodimeric Complex of Human RGS8 and Activated Gi Alpha 3 (pdb code 2ode). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Heterodimeric Complex of Human RGS8 and Activated Gi Alpha 3, PDB code: 2ode:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Heterodimeric Complex of Human RGS8 and Activated Gi Alpha 3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Heterodimeric Complex of Human RGS8 and Activated Gi Alpha 3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg3001 b:20.2 occ:1.00 F3 A:ALF2001 2.0 22.2 1.0 O1B A:GDP1001 2.0 22.3 1.0 O A:HOH3229 2.1 23.1 1.0 OG A:SER47 2.1 31.8 1.0 OG1 A:THR181 2.1 31.0 1.0 O A:HOH3231 2.1 19.9 1.0 CB A:THR181 3.1 33.6 1.0 CB A:SER47 3.2 31.5 1.0 PB A:GDP1001 3.2 19.4 1.0 F2 A:ALF2001 3.4 23.9 1.0 O2B A:GDP1001 3.4 18.8 1.0 AL A:ALF2001 3.5 20.4 1.0 N A:SER47 3.9 32.1 1.0 N A:THR181 4.0 32.4 1.0 CA A:SER47 4.1 31.8 1.0 OD2 A:ASP200 4.1 36.9 1.0 O A:HOH3093 4.1 30.1 1.0 CG2 A:THR181 4.1 34.3 1.0 CA A:THR181 4.2 33.0 1.0 O1A A:GDP1001 4.2 19.4 1.0 O3B A:GDP1001 4.3 21.2 1.0 OD1 A:ASP200 4.4 34.5 1.0 O3A A:GDP1001 4.4 20.1 1.0 F1 A:ALF2001 4.4 22.5 1.0 O A:VAL201 4.4 32.8 1.0 O A:VAL179 4.5 32.6 1.0 O A:HOH3236 4.6 18.5 1.0 CG A:ASP200 4.6 33.5 1.0 PA A:GDP1001 4.6 20.5 1.0 CB A:LYS46 4.7 31.9 1.0 O2A A:GDP1001 4.7 20.4 1.0 CE A:LYS46 4.8 31.7 1.0 O A:HOH3089 4.8 31.0 1.0 NH1 A:ARG178 4.9 34.5 1.0 C A:LYS46 5.0 32.2 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Heterodimeric Complex of Human RGS8 and Activated Gi Alpha 3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Heterodimeric Complex of Human RGS8 and Activated Gi Alpha 3 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg3002 b:24.7 occ:1.00 F1 C:ALF2002 2.0 24.6 1.0 O1B C:GDP1002 2.0 19.8 1.0 O C:HOH3190 2.1 21.4 1.0 OG1 C:THR181 2.2 32.1 1.0 OG C:SER47 2.2 30.8 1.0 O C:HOH3187 2.2 22.7 1.0 CB C:THR181 3.1 31.3 1.0 PB C:GDP1002 3.2 21.2 1.0 CB C:SER47 3.2 31.2 1.0 F4 C:ALF2002 3.3 27.4 1.0 O2B C:GDP1002 3.3 22.0 1.0 AL C:ALF2002 3.4 23.9 1.0 N C:SER47 3.9 32.3 1.0 N C:THR181 4.0 30.3 1.0 CA C:SER47 4.1 31.4 1.0 CG2 C:THR181 4.1 32.0 1.0 CA C:THR181 4.1 31.6 1.0 O C:HOH3019 4.2 25.7 1.0 O3B C:GDP1002 4.2 20.3 1.0 OD2 C:ASP200 4.2 34.2 1.0 O1A C:GDP1002 4.2 22.3 1.0 F3 C:ALF2002 4.3 22.0 1.0 O3A C:GDP1002 4.3 18.9 1.0 O C:VAL201 4.4 31.0 1.0 OD1 C:ASP200 4.4 34.8 1.0 O C:HOH3194 4.5 18.7 1.0 O C:VAL179 4.6 30.8 1.0 PA C:GDP1002 4.6 21.3 1.0 CG C:ASP200 4.7 32.7 1.0 CE C:LYS46 4.7 32.5 1.0 O C:HOH3045 4.8 33.5 1.0 O2A C:GDP1002 4.8 22.4 1.0 NH1 C:ARG178 4.8 33.1 1.0 CB C:LYS46 4.8 32.0 1.0 C C:LYS46 5.0 32.3 1.0 NZ C:LYS46 5.0 32.0 1.0

M.Soundararajan, F.S.Willard, A.J.Kimple, A.P.Turnbull, L.J.Ball, G.A.Schoch, C.Gileadi, O.Y.Fedorov, E.F.Dowler, V.A.Higman, S.Q.Hutsell, M.Sundstrom, D.A.Doyle, D.P.Siderovski. Structural Diversity in the Rgs Domain and Its Interaction with Heterotrimeric G Protein Alpha-Subunits. Proc.Natl.Acad.Sci.Usa V. 105 6457 2008.
ISSN: ISSN 0027-8424
PubMed: 18434541
DOI: 10.1073/PNAS.0801508105