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Magnesium in PDB 2oe5: 1.5 A X-Ray Crystal Structure of Apramycin Complex with Rna Fragment Ggcgucgcuaguaccg/Gguacuaaaagucgccc Containing the Human Ribosomal Decoding A Site: Rna Construct with 3'-Overhang

Protein crystallography data

The structure of 1.5 A X-Ray Crystal Structure of Apramycin Complex with Rna Fragment Ggcgucgcuaguaccg/Gguacuaaaagucgccc Containing the Human Ribosomal Decoding A Site: Rna Construct with 3'-Overhang, PDB code: 2oe5 was solved by T.Hermann, V.Tereshko, E.Skripkin, D.J.Patel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.92 / 1.51
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 28.310, 36.693, 86.679, 90.00, 90.00, 90.00
R / Rfree (%) 19.9 / 22.7

Magnesium Binding Sites:

The binding sites of Magnesium atom in the 1.5 A X-Ray Crystal Structure of Apramycin Complex with Rna Fragment Ggcgucgcuaguaccg/Gguacuaaaagucgccc Containing the Human Ribosomal Decoding A Site: Rna Construct with 3'-Overhang (pdb code 2oe5). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the 1.5 A X-Ray Crystal Structure of Apramycin Complex with Rna Fragment Ggcgucgcuaguaccg/Gguacuaaaagucgccc Containing the Human Ribosomal Decoding A Site: Rna Construct with 3'-Overhang, PDB code: 2oe5:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 2oe5

Go back to Magnesium Binding Sites List in 2oe5
Magnesium binding site 1 out of 2 in the 1.5 A X-Ray Crystal Structure of Apramycin Complex with Rna Fragment Ggcgucgcuaguaccg/Gguacuaaaagucgccc Containing the Human Ribosomal Decoding A Site: Rna Construct with 3'-Overhang


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of 1.5 A X-Ray Crystal Structure of Apramycin Complex with Rna Fragment Ggcgucgcuaguaccg/Gguacuaaaagucgccc Containing the Human Ribosomal Decoding A Site: Rna Construct with 3'-Overhang within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg104

b:39.7
occ:1.00
O A:HOH113 1.9 51.9 1.0
O A:HOH116 1.9 30.8 1.0
O A:HOH111 2.2 40.0 1.0
O A:HOH115 2.2 32.9 1.0
O A:HOH114 2.2 40.8 1.0
O A:HOH112 2.2 44.2 1.0
O A:HOH258 3.6 37.0 0.5
O A:HOH226 3.9 32.4 0.5
O6 A:G2 4.3 31.1 1.0
N7 A:G2 4.4 33.5 1.0

Magnesium binding site 2 out of 2 in 2oe5

Go back to Magnesium Binding Sites List in 2oe5
Magnesium binding site 2 out of 2 in the 1.5 A X-Ray Crystal Structure of Apramycin Complex with Rna Fragment Ggcgucgcuaguaccg/Gguacuaaaagucgccc Containing the Human Ribosomal Decoding A Site: Rna Construct with 3'-Overhang


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of 1.5 A X-Ray Crystal Structure of Apramycin Complex with Rna Fragment Ggcgucgcuaguaccg/Gguacuaaaagucgccc Containing the Human Ribosomal Decoding A Site: Rna Construct with 3'-Overhang within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg103

b:31.8
occ:1.00
O A:HOH109 2.0 32.7 1.0
O B:HOH108 2.0 33.0 1.0
O B:HOH106 2.0 30.8 1.0
O B:HOH107 2.1 33.1 1.0
O B:HOH110 2.2 35.0 1.0
O B:HOH105 2.2 29.7 1.0
O B:HOH145 3.8 31.3 1.0
O B:HOH204 3.9 24.0 0.5
O B:HOH118 4.0 22.6 1.0
O A:HOH142 4.1 31.1 1.0
O B:HOH120 4.2 23.1 1.0
O A:HOH213 4.3 26.3 0.5
O B:HOH134 4.5 29.7 1.0
OB3 B:AM2101 4.5 22.1 1.0
O B:HOH228 4.6 32.0 0.5
O A:HOH180 4.6 41.9 1.0
O4 B:U1489 4.6 20.3 1.0
N6 B:A1490 4.6 18.9 1.0
C5 B:U1489 4.8 20.3 1.0
N7 B:A1490 4.9 23.0 1.0
CB4 B:AM2101 5.0 20.6 1.0
O A:HOH196 5.0 47.8 1.0
C4 B:U1489 5.0 19.8 1.0

Reference:

T.Hermann, V.Tereshko, E.Skripkin, D.J.Patel. Apramycin Recognition By the Human Ribosomal Decoding Site. Blood Cells Mol.Dis. V. 38 193 2007.
ISSN: ISSN 1079-9796
PubMed: 17258916
DOI: 10.1016/J.BCMD.2006.11.006
Page generated: Wed Aug 14 01:26:21 2024

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