Magnesium in PDB 2oi5: E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa

All present enzymatic activity of E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa:
2.3.1.157; 2.7.7.23;

The structure of E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa, PDB code: 2oi5 was solved by L.R.Olsen, M.W.Vetting, S.L.Roderick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.87 / 2.25
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	102.487, 102.487, 644.426, 90.00, 90.00, 120.00
R / Rfree (%)	17.6 / 21.9

The binding sites of Magnesium atom in the E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa (pdb code 2oi5). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 5 binding sites of Magnesium where determined in the E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa, PDB code: 2oi5:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5;

Magnesium binding site 1 out of 5 in the E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg5003 b:16.8 occ:0.33 OD2 A:ASP406 2.0 21.7 1.0 CG A:ASP406 3.1 27.5 1.0 MG A:MG5004 3.6 25.6 0.3 OD1 A:ASP406 3.7 24.6 1.0 CB A:ASP406 4.2 16.7 1.0 O A:ASP406 4.2 19.5 1.0 O A:HOH7316 4.3 32.4 1.0 NE2 A:GLN408 5.0 37.2 1.0

Magnesium binding site 2 out of 5 in the E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg5004 b:25.6 occ:0.33 OD1 A:ASP406 1.9 24.6 1.0 O A:HOH7207 2.4 17.2 1.0 CG A:ASP406 2.7 27.5 1.0 OD2 A:ASP406 2.9 21.7 1.0 MG A:MG5003 3.6 16.8 0.3 CB A:ASP406 4.2 16.7 1.0 O A:GLY381 4.6 19.3 1.0 CA A:ASP406 4.7 17.6 1.0 O A:HOH7144 4.8 21.3 1.0 NE2 A:GLN408 4.8 37.2 1.0

Magnesium binding site 3 out of 5 in the E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg5000 b:27.4 occ:1.00 OD2 B:ASP105 2.1 16.4 1.0 O2B B:UD14001 2.2 15.6 1.0 O2A B:UD14001 2.2 17.5 1.0 OD1 B:ASN227 2.2 20.8 1.0 CG B:ASP105 3.1 20.9 1.0 CG B:ASN227 3.2 12.0 1.0 PA B:UD14001 3.3 17.4 1.0 OD1 B:ASP105 3.3 16.6 1.0 PB B:UD14001 3.4 20.6 1.0 O3A B:UD14001 3.5 16.6 1.0 ND2 B:ASN227 3.5 8.6 1.0 NZ B:LYS25 3.7 14.2 1.0 O6' B:UD14001 3.8 19.0 1.0 O5' B:UD14001 4.1 19.4 1.0 C5B B:UD14001 4.1 17.9 1.0 O5B B:UD14001 4.1 19.4 1.0 O B:VAL226 4.3 19.7 1.0 O1' B:UD14001 4.4 19.1 1.0 O1B B:UD14001 4.4 17.4 1.0 CB B:ASP105 4.4 15.2 1.0 O1A B:UD14001 4.4 21.7 1.0 CB B:ASN227 4.6 16.1 1.0 C1' B:UD14001 4.6 20.9 1.0 C B:VAL226 4.6 19.8 1.0 C5' B:UD14001 4.7 19.4 1.0 O B:HOH7015 4.7 15.5 1.0 N B:VAL226 4.7 19.8 1.0 C6' B:UD14001 4.8 23.6 1.0 CA B:ASN227 4.9 19.0 1.0 N B:ASN227 4.9 14.3 1.0 C3B B:UD14001 5.0 20.9 1.0

Magnesium binding site 4 out of 5 in the E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg5001 b:21.7 occ:0.33 OD1 B:ASP406 2.4 23.1 1.0 CG B:ASP406 3.6 30.1 1.0 O B:HOH7362 3.6 30.6 1.0 OD2 B:ASP406 4.1 32.4 1.0 O B:GLY381 4.4 18.4 1.0 O B:HOH7269 4.6 30.6 1.0 MG B:MG5002 4.6 33.8 0.3 CA B:GLY381 4.7 10.3 1.0 CB B:ASP406 4.8 18.2 1.0

Magnesium binding site 5 out of 5 in the E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of E. Coli Glmu- Complex with Udp-Glcnac and Acetyl-Coa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg5002 b:33.8 occ:0.33 OD2 B:ASP406 1.8 32.4 1.0 CG B:ASP406 2.8 30.1 1.0 OD1 B:ASP406 3.3 23.1 1.0 NE2 B:GLN408 3.6 35.6 1.0 CB B:ASP406 4.1 18.2 1.0 O B:ASP406 4.2 19.4 1.0 O B:HOH7626 4.5 40.7 1.0 MG B:MG5001 4.6 21.7 0.3 O B:HOH7269 4.7 30.6 1.0 CD B:GLN408 4.8 35.7 1.0

L.R.Olsen, M.W.Vetting, S.L.Roderick. Structure of the E. Coli Bifunctional Glmu Acetyltransferase Active Site with Substrates and Products. Protein Sci. V. 16 1230 2007.
ISSN: ISSN 0961-8368
PubMed: 17473010
DOI: 10.1110/PS.072779707