Magnesium in PDB 2pmz: Archaeal Rna Polymerase From Sulfolobus Solfataricus

All present enzymatic activity of Archaeal Rna Polymerase From Sulfolobus Solfataricus:
2.7.7.6;

The structure of Archaeal Rna Polymerase From Sulfolobus Solfataricus, PDB code: 2pmz was solved by K.S.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.79 / 3.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	125.820, 201.235, 196.050, 90.00, 100.92, 90.00
R / Rfree (%)	27.4 / 34.3

The structure of Archaeal Rna Polymerase From Sulfolobus Solfataricus also contains other interesting chemical elements:

Iron	(Fe)	6 atoms
Zinc	(Zn)	8 atoms

The binding sites of Magnesium atom in the Archaeal Rna Polymerase From Sulfolobus Solfataricus (pdb code 2pmz). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Archaeal Rna Polymerase From Sulfolobus Solfataricus, PDB code: 2pmz:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Archaeal Rna Polymerase From Sulfolobus Solfataricus


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Archaeal Rna Polymerase From Sulfolobus Solfataricus within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1003 b:57.7 occ:1.00 OD1 A:ASP460 2.5 60.0 1.0 OD1 A:ASP458 2.9 41.4 1.0 OD1 A:ASP456 3.1 40.3 1.0 OD2 A:ASP458 3.1 42.8 1.0 CG A:ASP460 3.3 59.2 1.0 OD2 A:ASP460 3.4 60.1 1.0 CG A:ASP458 3.4 42.3 1.0 OD2 A:ASP456 3.6 40.9 1.0 CG A:ASP456 3.8 40.4 1.0 NH1 A:ARG421 4.5 35.7 1.0 CB A:ASP460 4.7 58.3 1.0 CB A:ASP458 4.9 42.0 1.0

Magnesium binding site 2 out of 2 in the Archaeal Rna Polymerase From Sulfolobus Solfataricus


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Archaeal Rna Polymerase From Sulfolobus Solfataricus within 5.0Å range: probe atom residue distance (Å) B Occ Q:Mg1003 b:60.9 occ:1.00 OD1 Q:ASP456 2.4 81.7 1.0 OD1 Q:ASP458 2.5 71.1 1.0 OD1 Q:ASP460 2.8 58.3 1.0 OD2 Q:ASP456 2.8 83.1 1.0 OD2 Q:ASP458 2.9 72.7 1.0 CG Q:ASP456 3.0 82.4 1.0 CG Q:ASP458 3.0 73.3 1.0 OD2 Q:ASP460 3.2 57.4 1.0 CG Q:ASP460 3.3 57.6 1.0 CB Q:ASP456 4.5 82.2 1.0 CB Q:ASP458 4.5 74.2 1.0 NH1 Q:ARG421 4.6 64.4 1.0 CB Q:ASP460 4.7 57.0 1.0 O Q:ASP456 4.7 0.7 1.0 N Q:ASP456 4.9 0.5 1.0

A.Hirata, B.J.Klein, K.S.Murakami. The X-Ray Crystal Structure of Rna Polymerase From Archaea Nature V. 451 851 2008.
ISSN: ISSN 0028-0836
PubMed: 18235446
DOI: 10.1038/NATURE06530