Magnesium in PDB 2px0: Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+)

The structure of Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+), PDB code: 2px0 was solved by G.Bange, K.Wild, I.Sinning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	62.97 / 3.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	104.649, 111.376, 112.089, 90.00, 89.78, 90.00
R / Rfree (%)	25.4 / 32.8

The binding sites of Magnesium atom in the Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+) (pdb code 2px0). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 8 binding sites of Magnesium where determined in the Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+), PDB code: 2px0:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Magnesium binding site 1 out of 8 in the Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1000 b:47.7 occ:1.00 O A:HOH1001 2.1 44.3 1.0 O A:HOH1002 2.1 52.3 1.0 O2B A:GNP1 2.1 48.4 1.0 O A:HOH1003 2.1 45.2 1.0 O2G A:GNP1 2.1 45.6 1.0 OG1 A:THR189 2.3 46.3 1.0 PB A:GNP1 3.3 49.7 1.0 PG A:GNP1 3.3 44.6 1.0 N3B A:GNP1 3.5 46.4 1.0 CB A:THR189 3.6 45.8 1.0 OD2 A:ASP259 4.1 53.0 1.0 O3G A:GNP1 4.2 42.1 1.0 N A:THR189 4.3 43.1 1.0 O1B A:GNP1 4.3 47.2 1.0 OD1 A:ASP259 4.4 55.9 1.0 OD2 A:ASP213 4.4 63.0 1.0 O1G A:GNP1 4.4 42.1 1.0 O2A A:GNP1 4.4 50.6 1.0 O3A A:GNP1 4.5 50.6 1.0 CG2 A:THR189 4.5 45.7 1.0 NH1 A:ARG216 4.5 64.9 1.0 CA A:THR189 4.5 44.8 1.0 CG A:ASP259 4.7 55.3 1.0 O A:THR260 4.7 47.6 1.0 CD A:ARG216 4.8 62.2 1.0 PA A:GNP1 4.8 51.6 1.0 O1A A:GNP1 4.8 50.5 1.0

Magnesium binding site 2 out of 8 in the Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2000 b:51.3 occ:1.00 O B:HOH2002 2.1 55.8 1.0 O B:HOH2003 2.1 53.3 1.0 O2B B:GNP2 2.1 52.4 1.0 O B:HOH2001 2.1 50.6 1.0 O2G B:GNP2 2.1 52.8 1.0 OG1 B:THR189 2.4 60.6 1.0 CB B:THR189 3.3 61.6 1.0 PG B:GNP2 3.4 51.8 1.0 PB B:GNP2 3.4 53.5 1.0 N3B B:GNP2 3.5 51.6 1.0 N B:THR189 3.8 61.2 1.0 NH1 B:ARG216 4.2 67.3 1.0 CA B:THR189 4.2 62.9 1.0 O3G B:GNP2 4.2 47.3 1.0 OD1 B:ASP259 4.2 54.6 1.0 O2A B:GNP2 4.3 56.0 1.0 OD2 B:ASP213 4.3 74.2 1.0 OD2 B:ASP259 4.4 51.8 1.0 O1B B:GNP2 4.4 54.5 1.0 O3A B:GNP2 4.4 54.5 1.0 O1G B:GNP2 4.5 53.1 1.0 CG2 B:THR189 4.5 61.2 1.0 CB B:LYS188 4.7 58.0 1.0 PA B:GNP2 4.7 55.2 1.0 O1A B:GNP2 4.7 57.0 1.0 CG B:ASP259 4.8 55.4 1.0 O B:THR260 4.8 52.4 1.0 C B:LYS188 4.8 59.8 1.0 NZ B:LYS188 4.9 53.9 1.0

Magnesium binding site 3 out of 8 in the Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+) within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg3000 b:72.2 occ:1.00 O C:HOH3001 2.1 70.1 1.0 O C:HOH3002 2.1 71.9 1.0 O C:HOH3003 2.1 70.2 1.0 O2G C:GNP3 2.1 71.4 1.0 O2B C:GNP3 2.1 71.9 1.0 OG1 C:THR189 2.6 69.5 1.0 PG C:GNP3 3.3 71.4 1.0 PB C:GNP3 3.4 69.5 1.0 CB C:THR189 3.5 70.6 1.0 N3B C:GNP3 3.6 71.9 1.0 O3G C:GNP3 3.9 72.3 1.0 OD1 C:ASP259 4.0 85.8 1.0 OD2 C:ASP213 4.2 83.5 1.0 N C:THR189 4.3 69.3 1.0 O1B C:GNP3 4.3 72.3 1.0 O2A C:GNP3 4.4 76.2 1.0 OD2 C:ASP259 4.5 88.0 1.0 O1G C:GNP3 4.5 71.6 1.0 CA C:THR189 4.5 71.2 1.0 O3A C:GNP3 4.5 73.9 1.0 NH1 C:ARG216 4.5 91.3 1.0 O C:THR260 4.5 76.6 1.0 CG2 C:THR189 4.6 70.2 1.0 CE C:LYS188 4.6 51.3 1.0 CG C:ASP259 4.6 86.7 1.0 CD C:ARG216 4.7 86.5 1.0 PA C:GNP3 4.9 77.0 1.0

Magnesium binding site 4 out of 8 in the Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+) within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg4000 b:76.8 occ:1.00 O D:HOH402 2.1 77.9 1.0 O D:HOH401 2.1 75.5 1.0 O D:HOH403 2.1 76.7 1.0 O2B D:GNP4 2.1 77.7 1.0 O2G D:GNP4 2.1 77.7 1.0 OG1 D:THR189 2.6 78.6 1.0 CB D:THR189 3.0 80.9 1.0 PG D:GNP4 3.3 72.9 1.0 PB D:GNP4 3.3 77.2 1.0 N3B D:GNP4 3.5 74.6 1.0 N D:THR189 3.8 82.3 1.0 NZ D:LYS188 4.0 78.2 1.0 CA D:THR189 4.0 81.6 1.0 CG2 D:THR189 4.1 79.9 1.0 O3G D:GNP4 4.2 70.9 1.0 O2A D:GNP4 4.3 83.4 1.0 O1B D:GNP4 4.3 76.5 1.0 O1A D:GNP4 4.4 80.4 1.0 O1G D:GNP4 4.4 69.6 1.0 O3A D:GNP4 4.4 78.9 1.0 OD1 D:ASP259 4.5 94.6 1.0 PA D:GNP4 4.6 81.5 1.0 NH1 D:ARG216 4.6 83.3 1.0 CB D:LYS188 4.7 80.3 1.0 CD D:ARG216 4.7 81.7 1.0 OD2 D:ASP213 4.7 91.3 1.0 OD2 D:ASP259 4.8 92.8 1.0 C D:LYS188 5.0 82.0 1.0 CZ D:ARG216 5.0 83.4 1.0 NE D:ARG216 5.0 82.1 1.0

Magnesium binding site 5 out of 8 in the Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+) within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg5000 b:50.5 occ:1.00 O E:HOH502 2.1 51.6 1.0 O E:HOH503 2.1 51.0 1.0 O E:HOH501 2.1 50.3 1.0 O2B E:GNP5 2.1 49.7 1.0 O2G E:GNP5 2.1 49.7 1.0 OG1 E:THR189 2.6 48.7 1.0 CB E:THR189 3.3 47.0 1.0 PG E:GNP5 3.3 46.6 1.0 PB E:GNP5 3.3 48.4 1.0 N3B E:GNP5 3.5 47.9 1.0 N E:THR189 3.9 47.8 1.0 NH2 E:ARG216 4.0 49.9 1.0 OD2 E:ASP213 4.1 72.7 1.0 O3G E:GNP5 4.2 44.2 1.0 CA E:THR189 4.2 48.3 1.0 OD2 E:ASP259 4.2 67.7 1.0 O2A E:GNP5 4.3 54.5 1.0 O1B E:GNP5 4.3 49.0 1.0 O1G E:GNP5 4.4 48.7 1.0 OD1 E:ASP259 4.4 67.5 1.0 O3A E:GNP5 4.4 48.7 1.0 CG2 E:THR189 4.5 43.6 1.0 CB E:LYS188 4.5 48.1 1.0 OE1 E:GLN222 4.6 84.5 1.0 NZ E:LYS188 4.7 37.9 1.0 PA E:GNP5 4.7 52.4 1.0 O E:THR260 4.8 60.4 1.0 CG E:ASP259 4.8 67.1 1.0 O1A E:GNP5 4.9 54.5 1.0 C E:LYS188 5.0 48.5 1.0

Magnesium binding site 6 out of 8 in the Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+) within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg6000 b:47.3 occ:1.00 O F:HOH602 2.1 50.9 1.0 O F:HOH603 2.1 46.5 1.0 O F:HOH601 2.1 44.4 1.0 O2B F:GNP6 2.1 48.8 1.0 O2G F:GNP6 2.1 50.8 1.0 OG1 F:THR189 2.2 52.0 1.0 PB F:GNP6 3.4 49.6 1.0 PG F:GNP6 3.4 49.4 1.0 CB F:THR189 3.5 50.7 1.0 N3B F:GNP6 3.5 49.3 1.0 N F:THR189 3.9 49.6 1.0 NZ F:LYS188 4.0 47.8 1.0 OD2 F:ASP259 4.0 54.9 1.0 OD1 F:ASP259 4.2 56.2 1.0 OD2 F:ASP213 4.2 78.4 1.0 O3G F:GNP6 4.2 49.1 1.0 CA F:THR189 4.2 50.2 1.0 O1B F:GNP6 4.3 49.4 1.0 NH1 F:ARG216 4.4 82.8 1.0 O1G F:GNP6 4.4 51.1 1.0 O3A F:GNP6 4.5 55.7 1.0 O2A F:GNP6 4.5 58.5 1.0 CB F:LYS188 4.5 50.8 1.0 O F:THR260 4.5 54.8 1.0 CG2 F:THR189 4.5 51.0 1.0 CG F:ASP259 4.5 56.4 1.0 PA F:GNP6 4.8 58.5 1.0 O1A F:GNP6 5.0 60.2 1.0

Magnesium binding site 7 out of 8 in the Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+) within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg7000 b:59.9 occ:1.00 O G:HOH701 2.1 59.4 1.0 O G:HOH702 2.1 62.4 1.0 O G:HOH703 2.1 58.7 1.0 O2B G:GNP7 2.1 61.7 1.0 O2G G:GNP7 2.1 61.9 1.0 OG1 G:THR189 2.6 55.0 1.0 CB G:THR189 3.2 59.2 1.0 PG G:GNP7 3.4 58.7 1.0 PB G:GNP7 3.4 63.3 1.0 N3B G:GNP7 3.6 60.8 1.0 NH1 G:ARG216 3.7 89.6 1.0 N G:THR189 3.9 62.5 1.0 OD2 G:ASP213 3.9 78.8 1.0 O3G G:GNP7 4.1 60.1 1.0 CA G:THR189 4.1 62.1 1.0 O2A G:GNP7 4.1 64.6 1.0 OD2 G:ASP259 4.3 80.4 1.0 O1B G:GNP7 4.4 60.5 1.0 O3A G:GNP7 4.4 64.4 1.0 CG2 G:THR189 4.4 56.5 1.0 O G:THR260 4.5 63.2 1.0 O1G G:GNP7 4.5 55.5 1.0 PA G:GNP7 4.6 66.5 1.0 OD1 G:ASP259 4.8 78.2 1.0 CB G:LYS188 4.8 60.9 1.0 O1A G:GNP7 4.8 66.3 1.0 CE G:LYS188 4.9 51.2 1.0 CG G:ASP213 4.9 78.9 1.0 CG G:ASP259 5.0 77.7 1.0

Magnesium binding site 8 out of 8 in the Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 8 of Crystal Structure of Flhf Complexed with Gmppnp/Mg(2+) within 5.0Å range: probe atom residue distance (Å) B Occ H:Mg8000 b:74.2 occ:1.00 O H:HOH801 2.1 72.4 1.0 O H:HOH803 2.1 71.7 1.0 O H:HOH802 2.1 73.0 1.0 O2B H:GNP8 2.1 73.0 1.0 O2G H:GNP8 2.1 74.1 1.0 OG1 H:THR189 2.7 70.8 1.0 PG H:GNP8 3.3 73.0 1.0 PB H:GNP8 3.4 73.4 1.0 CB H:THR189 3.6 70.8 1.0 N3B H:GNP8 3.6 72.9 1.0 NH1 H:ARG216 3.8 0.1 1.0 OE1 H:GLN222 4.0 0.7 1.0 O3G H:GNP8 4.0 73.7 1.0 OD2 H:ASP259 4.1 88.5 1.0 N H:THR189 4.3 71.8 1.0 O2A H:GNP8 4.3 74.8 1.0 O1B H:GNP8 4.4 72.7 1.0 OD2 H:ASP213 4.4 0.5 1.0 O3A H:GNP8 4.5 73.9 1.0 O1G H:GNP8 4.5 73.0 1.0 CA H:THR189 4.5 71.1 1.0 NH2 H:ARG216 4.6 0.4 1.0 CG2 H:THR189 4.6 70.8 1.0 CZ H:ARG216 4.7 0.4 1.0 OD1 H:ASP259 4.7 88.3 1.0 CD H:GLN222 4.8 0.3 1.0 O H:THR260 4.8 80.6 1.0 NE2 H:GLN222 4.8 0.9 1.0 CG H:ASP259 4.8 88.0 1.0 PA H:GNP8 4.9 72.6 1.0 CE H:LYS188 4.9 68.8 1.0

G.Bange, G.Petzold, K.Wild, R.O.Parlitz, I.Sinning. The Crystal Structure of the Third Signal-Recognition Particle Gtpase Flhf Reveals A Homodimer with Bound Gtp. Proc.Natl.Acad.Sci.Usa V. 104 13621 2007.
ISSN: ISSN 0027-8424
PubMed: 17699634
DOI: 10.1073/PNAS.0702570104