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Magnesium in PDB 2pyj: PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex)
Enzymatic activity of PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex)
All present enzymatic activity of PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex):
2.7.7.7;
2.7.7.7;
Protein crystallography data
The structure of PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex), PDB code: 2pyj
was solved by
A.J.Berman,
S.Kamtekar,
J.L.Goodman,
J.M.Lazaro,
M.De Vega,
L.Blanco,
M.Salas,
T.A.Steitz,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
| Resolution Low / High (Å) | 41.07 / 2.03 |
| Space group | P 1 21 1 |
| Cell size a, b, c (Å), α, β, γ (°) | 72.835, 114.667, 104.761, 90.00, 94.07, 90.00 |
| R / Rfree (%) | 18.9 / 23.4 |
Other elements in 2pyj:
The structure of PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex) also contains other interesting chemical elements:
| Manganese | (Mn) | 2 atoms |
Magnesium Binding Sites:
The binding sites of Magnesium atom in the PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex)
(pdb code 2pyj). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex), PDB code: 2pyj:
Jump to Magnesium binding site number: 1; 2;
In total 2 binding sites of Magnesium where determined in the PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex), PDB code: 2pyj:
Jump to Magnesium binding site number: 1; 2;
Magnesium binding site 1 out of 2 in 2pyj
Go back to
Magnesium Binding Sites List in 2pyj
Magnesium binding site 1 out
of 2 in the PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex)
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex) within 5.0Å range:
|
Magnesium binding site 2 out of 2 in 2pyj
Go back to
Magnesium Binding Sites List in 2pyj
Magnesium binding site 2 out
of 2 in the PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex)
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of PHI29 Dna Polymerase Complexed with Primer-Template Dna and Incoming Nucleotide Substrates (Ternary Complex) within 5.0Å range:
|
Reference:
A.J.Berman,
S.Kamtekar,
J.L.Goodman,
J.M.Lazaro,
M.De Vega,
L.Blanco,
M.Salas,
T.A.Steitz.
Structures of PHI29 Dna Polymerase Complexed with Substrate: the Mechanism of Translocation in B-Family Polymerases Embo J. V. 26 3494 2007.
ISSN: ISSN 0261-4189
PubMed: 17611604
DOI: 10.1038/SJ.EMBOJ.7601780
Page generated: Mon Dec 14 07:35:23 2020
ISSN: ISSN 0261-4189
PubMed: 17611604
DOI: 10.1038/SJ.EMBOJ.7601780
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