Magnesium in PDB 2q0d: Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Atp

All present enzymatic activity of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Atp:
2.7.7.52;

The structure of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Atp, PDB code: 2q0d was solved by J.Stagno, H.Luecke, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.38 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	80.190, 41.970, 106.420, 90.00, 94.01, 90.00
R / Rfree (%)	22.1 / 25.4

The binding sites of Magnesium atom in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Atp (pdb code 2q0d). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Atp, PDB code: 2q0d:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Atp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Atp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:10.3 occ:1.00 O3G A:ATP501 2.1 20.7 1.0 OD2 A:ASP66 2.3 20.5 1.0 OD2 A:ASP68 2.3 14.7 1.0 O A:HOH507 2.3 17.9 1.0 O1B A:ATP501 2.4 22.6 1.0 O A:HOH664 2.5 18.8 1.0 O1A A:ATP501 2.6 20.2 1.0 CG A:ASP68 3.4 17.5 1.0 CG A:ASP66 3.4 21.7 1.0 PB A:ATP501 3.5 19.2 1.0 PG A:ATP501 3.5 17.0 1.0 OD1 A:ASP68 3.7 20.1 1.0 OD1 A:ASP66 3.7 20.4 1.0 O3B A:ATP501 3.8 20.2 1.0 PA A:ATP501 3.8 19.2 1.0 MG A:MG402 3.9 19.3 1.0 O A:HOH556 3.9 30.6 1.0 O3A A:ATP501 4.0 21.1 1.0 O A:HOH615 4.1 27.5 1.0 C5' A:ATP501 4.3 22.1 1.0 O2G A:ATP501 4.4 21.6 1.0 O A:HOH636 4.5 30.8 1.0 O5' A:ATP501 4.5 20.7 1.0 O1G A:ATP501 4.5 20.5 1.0 O A:ASP66 4.6 20.8 1.0 OG A:SER54 4.6 16.8 1.0 O A:HOH514 4.6 20.5 1.0 N A:SER54 4.7 14.0 1.0 CB A:ASP68 4.7 18.1 1.0 C A:ASP66 4.7 21.5 1.0 CB A:ASP66 4.8 21.9 1.0 CA A:GLY53 4.8 12.3 1.0 O2B A:ATP501 4.8 18.3 1.0 O A:HOH665 4.9 34.0 1.0 N A:ASP68 4.9 20.0 1.0 N A:ASP66 5.0 23.8 1.0

Magnesium binding site 2 out of 2 in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Atp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Atp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:19.3 occ:1.00 O A:HOH665 2.3 34.0 1.0 O A:HOH671 2.4 38.2 1.0 O1A A:ATP501 2.5 20.2 1.0 O A:HOH507 2.6 17.9 1.0 O A:HOH515 2.6 17.0 1.0 O A:HOH636 2.6 30.8 1.0 O A:HOH615 2.7 27.5 1.0 O A:HOH670 2.9 43.2 1.0 PA A:ATP501 3.6 19.2 1.0 O2A A:ATP501 3.8 19.9 1.0 MG A:MG401 3.9 10.3 1.0 OD1 A:ASP66 4.2 20.4 1.0 O3G A:ATP501 4.3 20.7 1.0 O A:HOH668 4.3 41.9 1.0 O A:HOH640 4.3 25.1 1.0 O5' A:ATP501 4.4 20.7 1.0 O A:HOH669 4.5 35.0 1.0 OD2 A:ASP66 4.6 20.5 1.0 OD1 A:ASP68 4.7 20.1 1.0 O A:HOH531 4.7 21.5 1.0 OD2 A:ASP136 4.8 23.6 1.0 CG A:ASP66 4.8 21.7 1.0 O A:HOH556 4.9 30.6 1.0 O3A A:ATP501 4.9 21.1 1.0 C5' A:ATP501 4.9 22.1 1.0 O1G A:ATP501 4.9 20.5 1.0

J.Stagno, I.Aphasizheva, R.Aphasizhev, H.Luecke. Dual Role of the Rna Substrate in Selectivity and Catalysis By Terminal Uridylyl Transferases. Proc.Natl.Acad.Sci.Usa V. 104 14634 2007.
ISSN: ISSN 0027-8424
PubMed: 17785418
DOI: 10.1073/PNAS.0704259104