Magnesium in PDB 2q0e: Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Gtp

All present enzymatic activity of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Gtp:
2.7.7.52;

The structure of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Gtp, PDB code: 2q0e was solved by J.Stagno, H.Luecke, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.60 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	80.788, 41.917, 106.388, 90.00, 93.34, 90.00
R / Rfree (%)	23.2 / 28

The binding sites of Magnesium atom in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Gtp (pdb code 2q0e). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Gtp, PDB code: 2q0e:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Gtp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Gtp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:13.5 occ:0.65 O3G A:GTP501 2.3 23.4 0.7 O A:HOH649 2.4 27.9 1.0 O1B A:GTP501 2.4 20.5 0.7 O1A A:GTP501 2.4 24.7 0.7 OD2 A:ASP66 2.4 38.2 1.0 O A:HOH519 2.5 29.4 1.0 OD2 A:ASP68 2.7 32.8 1.0 CG A:ASP66 3.5 38.8 1.0 PB A:GTP501 3.5 25.1 0.7 PG A:GTP501 3.6 23.0 0.7 PA A:GTP501 3.7 24.6 0.7 CG A:ASP68 3.7 31.3 1.0 O A:HOH551 3.7 32.4 1.0 MG A:MG402 3.8 23.9 0.7 OD1 A:ASP66 3.8 38.3 1.0 O3B A:GTP501 3.9 25.2 0.7 OD1 A:ASP68 4.0 35.1 1.0 O3A A:GTP501 4.0 25.0 0.7 C5' A:GTP501 4.2 25.9 0.7 O A:HOH651 4.3 45.1 1.0 O5' A:GTP501 4.3 26.8 0.7 O2G A:GTP501 4.6 26.4 0.7 O1G A:GTP501 4.6 24.3 0.7 O A:ASP66 4.7 36.6 1.0 N A:SER54 4.7 21.4 1.0 O A:HOH605 4.8 44.5 1.0 O2B A:GTP501 4.8 22.8 0.7 N A:ASP66 4.9 40.3 1.0 O2A A:GTP501 4.9 22.5 0.7 CB A:ASP66 4.9 38.9 1.0 CA A:GLY53 4.9 20.3 1.0 O A:HOH546 4.9 28.4 1.0 OG A:SER54 4.9 22.6 1.0 C A:ASP66 5.0 37.1 1.0

Magnesium binding site 2 out of 2 in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Gtp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Gtp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:23.9 occ:0.65 O A:HOH651 2.4 45.1 1.0 O A:HOH605 2.4 44.5 1.0 O A:HOH650 2.5 45.9 1.0 O1A A:GTP501 2.5 24.7 0.7 O A:HOH540 2.5 27.6 1.0 O A:HOH519 2.7 29.4 1.0 PA A:GTP501 3.5 24.6 0.7 O2A A:GTP501 3.6 22.5 0.7 MG A:MG401 3.8 13.5 0.7 O5' A:GTP501 4.2 26.8 0.7 O3G A:GTP501 4.4 23.4 0.7 OD1 A:ASP66 4.4 38.3 1.0 O A:HOH588 4.5 28.9 1.0 OD2 A:ASP66 4.8 38.2 1.0 O A:HOH532 4.8 21.1 1.0 OD1 A:ASP68 4.9 35.1 1.0 O3A A:GTP501 4.9 25.0 0.7 O A:HOH551 4.9 32.4 1.0 C5' A:GTP501 5.0 25.9 0.7 OD2 A:ASP136 5.0 33.5 1.0

J.Stagno, I.Aphasizheva, R.Aphasizhev, H.Luecke. Dual Role of the Rna Substrate in Selectivity and Catalysis By Terminal Uridylyl Transferases. Proc.Natl.Acad.Sci.Usa V. 104 14634 2007.
ISSN: ISSN 0027-8424
PubMed: 17785418
DOI: 10.1073/PNAS.0704259104