Magnesium in PDB 2q0g: Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Upu

All present enzymatic activity of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Upu:
2.7.7.52;

The structure of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Upu, PDB code: 2q0g was solved by J.Stagno, H.Luecke, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.52 / 2.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	81.544, 41.953, 106.593, 90.00, 95.62, 90.00
R / Rfree (%)	22.8 / 29.6

The structure of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Upu also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Magnesium atom in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Upu (pdb code 2q0g). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Upu, PDB code: 2q0g:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Upu


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Upu within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:28.6 occ:1.00 OD2 A:ASP136 2.0 37.6 1.0 OD1 A:ASP68 2.3 30.3 1.0 OD1 A:ASP66 2.7 51.8 1.0 O1 A:UPU501 2.7 34.0 1.0 O A:HOH552 2.9 43.0 1.0 CG A:ASP68 3.1 29.6 1.0 OD2 A:ASP66 3.2 52.3 1.0 CG A:ASP136 3.2 36.0 1.0 OD2 A:ASP68 3.2 32.6 1.0 CG A:ASP66 3.3 50.6 1.0 O3'B A:UPU501 3.5 32.8 1.0 P A:UPU501 3.7 34.1 1.0 OD1 A:ASP136 4.0 39.8 1.0 CB A:ASP136 4.2 34.4 1.0 O A:VAL67 4.4 33.8 1.0 CB A:ASP68 4.4 28.5 1.0 O2'B A:UPU501 4.5 33.8 1.0 CB A:ASP66 4.6 47.9 1.0 O5'A A:UPU501 4.7 30.6 1.0 C3'B A:UPU501 4.7 35.7 1.0 C A:VAL67 4.7 34.0 1.0 N A:ASP136 4.7 34.4 1.0 C5'A A:UPU501 4.8 26.6 1.0 CA A:ASP68 4.8 28.8 1.0 NH2 A:ARG126 4.8 52.3 1.0 N A:ASP68 4.9 31.0 1.0

Magnesium binding site 2 out of 2 in the Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Upu


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Terminal Uridylyl Transferase 4 From Trypanosoma Brucei with Bound Upu within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg402 b:45.5 occ:1.00 OD1 B:ASP66 1.8 67.5 1.0 OD1 B:ASP68 2.2 54.5 1.0 O1 B:UPU502 2.6 50.8 1.0 CG B:ASP66 2.7 66.9 1.0 OD1 B:ASP136 2.8 64.5 1.0 CG B:ASP68 3.0 55.2 1.0 OD2 B:ASP66 3.1 68.8 1.0 O3'B B:UPU502 3.1 48.8 1.0 OD2 B:ASP68 3.1 53.2 1.0 CG B:ASP136 3.4 64.2 1.0 P B:UPU502 3.5 49.9 1.0 CB B:ASP66 3.9 65.7 1.0 CB B:ASP136 4.0 64.2 1.0 OD2 B:ASP136 4.0 62.9 1.0 O2'B B:UPU502 4.2 51.5 1.0 O B:HOH535 4.3 53.5 1.0 CB B:ASP68 4.4 55.8 1.0 C5'A B:UPU502 4.4 45.2 1.0 C3'B B:UPU502 4.4 50.0 1.0 O5'A B:UPU502 4.5 46.9 1.0 O B:VAL67 4.6 59.0 1.0 N B:ASP136 4.7 65.3 1.0 C B:ASP66 4.7 64.2 1.0 O2 B:UPU502 4.7 49.1 1.0 O B:ASP66 4.8 64.3 1.0 C B:VAL67 4.8 59.4 1.0 CA B:ASP66 4.9 65.5 1.0 C2'B B:UPU502 4.9 49.9 1.0 CA B:ASP68 4.9 55.8 1.0 N B:ASP68 5.0 57.6 1.0

J.Stagno, I.Aphasizheva, R.Aphasizhev, H.Luecke. Dual Role of the Rna Substrate in Selectivity and Catalysis By Terminal Uridylyl Transferases. Proc.Natl.Acad.Sci.Usa V. 104 14634 2007.
ISSN: ISSN 0027-8424
PubMed: 17785418
DOI: 10.1073/PNAS.0704259104