Magnesium in PDB 2q66: Structure of Yeast Poly(A) Polymerase with Atp and Oligo(A)

All present enzymatic activity of Structure of Yeast Poly(A) Polymerase with Atp and Oligo(A):
2.7.7.19;

The structure of Structure of Yeast Poly(A) Polymerase with Atp and Oligo(A), PDB code: 2q66 was solved by A.Bohm, P.Balbo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.71 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	67.633, 85.907, 107.469, 90.00, 90.00, 90.00
R / Rfree (%)	18.9 / 22.5

The binding sites of Magnesium atom in the Structure of Yeast Poly(A) Polymerase with Atp and Oligo(A) (pdb code 2q66). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Structure of Yeast Poly(A) Polymerase with Atp and Oligo(A), PDB code: 2q66:

Magnesium binding site 1 out of 1 in the Structure of Yeast Poly(A) Polymerase with Atp and Oligo(A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Yeast Poly(A) Polymerase with Atp and Oligo(A) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg602 b:19.6 occ:1.00 O1G A:ATP605 2.0 22.1 1.0 OD1 A:ASP100 2.0 19.2 1.0 O1B A:ATP605 2.1 18.5 1.0 O1A A:ATP605 2.1 21.9 1.0 OD2 A:ASP102 2.1 17.8 1.0 O A:HOH1097 2.2 19.0 1.0 CG A:ASP100 3.1 20.6 1.0 CG A:ASP102 3.2 17.9 1.0 PB A:ATP605 3.2 20.2 1.0 PA A:ATP605 3.3 23.2 1.0 PG A:ATP605 3.3 20.6 1.0 OD2 A:ASP100 3.5 21.3 1.0 OD1 A:ASP102 3.5 19.2 1.0 O3A A:ATP605 3.5 21.2 1.0 O3B A:ATP605 3.6 20.8 1.0 O A:HOH817 3.9 21.0 1.0 OG A:SER89 4.0 19.7 1.0 O A:HOH725 4.0 15.6 1.0 O A:ASP100 4.1 19.1 1.0 O3G A:ATP605 4.1 20.4 1.0 C5' A:ATP605 4.1 22.5 1.0 O5' A:ATP605 4.3 23.4 1.0 N A:SER89 4.3 18.4 1.0 O2G A:ATP605 4.4 22.9 1.0 CB A:ASP100 4.5 18.9 1.0 CB A:ASP102 4.5 17.0 1.0 O2A A:ATP605 4.5 21.8 1.0 C A:ASP100 4.5 19.3 1.0 O2B A:ATP605 4.6 20.3 1.0 N A:ASP100 4.6 20.1 1.0 CA A:ASP100 4.7 19.6 1.0 CA A:GLY88 4.7 17.9 1.0 N A:ASP102 4.7 17.4 1.0 O X:HOH738 4.8 38.3 1.0 O3' X:A5 4.8 20.9 1.0 CB A:SER89 4.9 19.4 1.0

P.B.Balbo, A.Bohm. Mechanism of Poly(A) Polymerase: Structure of the Enzyme-Mgatp-Rna Ternary Complex and Kinetic Analysis. Structure V. 15 1117 2007.
ISSN: ISSN 0969-2126
PubMed: 17850751
DOI: 10.1016/J.STR.2007.07.010